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Species: Gallus gallus
Genetic Insert: Thyroid Response Element
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32612 Copy
Species: Gallus gallus
Genetic Insert: rGH-PAL
Vector Backbone Description: Backbone Marker:Mark Emerson; Backbone Size:6610; Vector Backbone:Stagia3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32617 Copy
Species: Gallus gallus
Genetic Insert: GAPDH shRNA #2
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32622 Copy
Species: Gallus gallus
Genetic Insert: TRbeta shRNA #1
Vector Backbone Description: Vector Backbone:pRFP RNAiC-1; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21060789
Proper citation: RRID:Addgene_32620 Copy
Species: Gallus gallus
Genetic Insert: ASIC1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1 TOPO; Vector Types:Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_32791 Copy
Species: Gallus gallus
Genetic Insert: AP-SEMA3C
Vector Backbone Description: Backbone Marker:modified from PCDNA3 see methods in associated publication.; Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9331347
Proper citation: RRID:Addgene_29449 Copy
Species: Gallus gallus
Genetic Insert: AP-SEMA3A (chick)
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9331347
Proper citation: RRID:Addgene_29448 Copy
Species: Gallus gallus
Genetic Insert: SEMA3A (chick)
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3000; Vector Backbone:pBLUESCRIPT (SK); Vector Types:pBlue for ISH; Bacterial Resistance:Ampicillin
Comments: To generate the sense probe for ISH, linearize the plasmid with XbaI and transcribe with T3 RNA polymerase. To generate the antisense probe, linearize the plasmid with NotI and transcribe with T7 RNA polymerase.
Please note that T7 is the 5' sequencing primer for the vector; however, the SEMA3A insert is cloned in reverse orientation. For cloning purposes, NotI is at the 5' end of SEMA3A while XbaI is at the 3' end of SEMA3A.
Proper citation: RRID:Addgene_29564 Copy
Species: Gallus gallus
Genetic Insert: paxillin-Electra2
Vector Backbone Description: Backbone Size:3933; Vector Backbone:pN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35715437
Proper citation: RRID:Addgene_184926 Copy
Species: Gallus gallus
Genetic Insert: Paxillin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pLenti6.3/V5-DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23990882
Proper citation: RRID:Addgene_187688 Copy
Species: Gallus gallus
Genetic Insert: Myr-c-P3k-FLAG
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCAS(BP-A); Vector Types:Retroviral, avian; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9188528
Proper citation: RRID:Addgene_196373 Copy
Species: Gallus gallus
Genetic Insert: CXADR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA6/myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_87232 Copy
Species: Gallus gallus
Genetic Insert: FRNK
Vector Backbone Description: Backbone Marker:GE-Healthcare; Backbone Size:12769; Vector Backbone:TRIPZ-non-silencing shRNA control; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26216901
Proper citation: RRID:Addgene_83472 Copy
Species: Gallus gallus
Genetic Insert: CSPG5
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pGBT9; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89402 Copy
Species: Gallus gallus
Genetic Insert: CNTN1
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89412 Copy
Species: Gallus gallus
Genetic Insert: RSK
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11585927
Comments: Src myristylation sequence (MGSSKSKPK) lacks the polybasic domain necessary for predominant membrane localization.
Proper citation: RRID:Addgene_8997 Copy
Species: Gallus gallus
Genetic Insert: VinculinCS
Vector Backbone Description: Backbone Size:4644; Vector Backbone:pRRL; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36924770
Proper citation: RRID:Addgene_200309 Copy
Species: Gallus gallus
Genetic Insert: Vinculin_TurboID_Venus
Vector Backbone Description: Backbone Size:5379; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36924770
Proper citation: RRID:Addgene_200272 Copy
Species: Gallus gallus
Genetic Insert: VinculinI997A_TurboID_Venus
Vector Backbone Description: Backbone Size:5380; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36924770
Proper citation: RRID:Addgene_200274 Copy
Species: Gallus gallus
Genetic Insert: Ovalbumin
Vector Backbone Description: Backbone Marker:Broad Institute Genetic Perturbation Platform; Backbone Size:7555; Vector Backbone:pLX_305; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38411617
Proper citation: RRID:Addgene_184924 Copy
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