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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
M2371 his3::ADE2 Disruptor Converter
 
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RRID:Addgene_51671 ADE2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid was made by cutting pJJ215 with MscI and Nsi I and replacing the 570 bp MscI–Nsi I fragment with a 3.6 kb NotI (blunted with Klenow)–PstI fragment containing ADE2 from plasmid M1144. Plasmid M1144 contains a 3.6 kb BglII fragment (partial digest product) with ADE2 with BamHI linkers cloned into the BamHI site of Bluescript KS+. Vector Backbone:pJJ215; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
M3499 ura3::ADE2 Disruptor Converter
 
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RRID:Addgene_51674 ADE2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid M3499 (ura3::ADE2 ) was made by cutting pJJ244 with StuI and EcoRV and replacing the 248 bp StuI–EcoRV fragment with a 3.6 kb BamHI blunted with Klenow) fragment containing ADE2 from plasmid M1144. Vector Backbone:pJJ244; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 1
pExp_TRE:TAL21
 
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RRID:Addgene_51795 TAL21 Ampicillin PMID:25369267 Vector Backbone:pZDONOR 5-6; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:17 0
pExp_CAG:EBFP2
 
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RRID:Addgene_51790 EBFP2 Synthetic Ampicillin PMID:25369267 Vector Backbone:pZDONOR 5-6; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:16:17 0
D1433 his3::LYS2 Disruptor Converter
 
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RRID:Addgene_51676 LYS2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid was made by cutting YDp-H with BglII and replacing the 60 bp BglII fragment with a 5 kb BamHI fragment (partial digest product) containing LYS2 from plasmid YDp-K. Vector Backbone:YDp-H; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
M2660 ura3::LYS2 Disruptor Converter
 
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RRID:Addgene_51678 LYS2 Saccharomyces cerevisiae Ampicillin PMID:12898713 Plasmid M2660 (ura3::LYS2 ) was made by cutting M581 with EcoRV and PstI and replacing the 208 bp EcoRV–PstI fragment with a 4.6 kb PvuII–PstI fragment containing LYS2 from plasmid YDp-K. Backbone Marker:Marsh et al., 1984; Vector Backbone:M581; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
pC-PTP-NEO
 
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RRID:Addgene_51710 Ampicillin PMID:16278461 The PTP tag is a derivative of the original TAP tag and has CBP replaced by ProtC. Gene flanks providing RNA processing signals for PTP fusion and resistance marker are the 3′ flank of TbRPA1 (RPA1-3′), HSP70 genes 2 and 3 intergenic region (H23), the β-α tubulin intergenic region (T), and the 5′ flank of TbRPA2 (RPA2-5′). pC-PTP-NEO contains a target sequence (U1-70K) which can be replaced by the gene of interest. Vector Backbone:pBluescript KS+; Vector Types:T. brucei genome integration vectors; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
pFRT/FLAG/HA MOV10 K530A
 
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RRID:Addgene_51716 MOV10 K530A Homo sapiens Ampicillin PMID:24726324 Backbone Size:5299; Vector Backbone:pFRT/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K530A 2026-08-15 01:16:16 0
M4755 KanMX::LEU2 Disruptor Converter
 
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RRID:Addgene_51685 LEU2 Saccharomyces cerevisiae Ampicillin PMID:12898713 M4756 (kanMX::LEU2)was made by digesting M4757 with BamHI and SphI and inserting a 2.0 kb BamHI–SphI fragment with LEU2 from plasmid pJJ250. Vector Backbone:M4757 (kanMX::TRP1), Addgene plasmid #51687; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
M3926 leu2::KanMX3 Disruptor Converter
 
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RRID:Addgene_51680 KanMX3 Ampicillin PMID:12898713 Plasmid M3926 (leu2::KanMX3 ) was made by cutting YDp-L with EcoRV and inserting a 2.5 kb BglII–EcoRI (both ends blunted with Klenow)fragment containing KanMX3 from plasmid pFA6–KanMX3. Vector Backbone:YDp-L; Vector Types:Yeast Expression, yeast marker swap; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 1
pDEST myc MOV10 D645N
 
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RRID:Addgene_51720 MOV10 D645N Homo sapiens Ampicillin PMID:24726324 Backbone Marker:Invitrogen; Backbone Size:5882; Vector Backbone:pDEST26; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin D645N 2026-08-15 01:16:16 0
pcDNA3.1-aDTX-lumitoxin
 
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RRID:Addgene_51686 aDTX-lumitoxin Synthetic Ampicillin PMID:24407101 Backbone Size:5341; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
SpyLigase
 
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RRID:Addgene_51722 SpyLigase Synthetic Ampicillin PMID:24639550 SnoopLigase is the more recent peptide-peptide ligation technology from the Howarth lab and is now preferred over SpyLigase in terms of more general reaction conditions and higher coupling yield on a range of proteins. pET28a-AviTag-SnoopLigase https://www.addgene.org/105626/ pET28a-HaloTag7-SnoopLigase https://www.addgene.org/105627/ SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Jacob O. Fierer, Gianluca Veggiani and Mark Howarth Transform plasmid into BL21 DE3 RIPL for expression. Backbone Marker:Life Technologies; Backbone Size:6400; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 2
p18S.1 G693A
 
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RRID:Addgene_51728 18S Mus musculus Ampicillin PMID:22718970 Please note that the depositor sequence is theoretical. Addgene verified that the nucleotides A5152 (nucleotide 963 in 18 S rRNA) and G5162 are expected in 18S, based on the publication and GenBank ID: JQ247698.1. Please see Addgene's result using the m18Sinternal-rev primer in the "Sequences" link above. Backbone Marker:promega; Backbone Size:2000; Vector Backbone:pCMV; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:16:20 0
DRH296: FCK-Optopatch2
 
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RRID:Addgene_51694 Optopatch2 Synthetic Ampicillin PMID:24952910 Backbone Marker:Pavel Osten; Backbone Size:9236; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 8
DRH335: FCK-Optopatch1
 
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RRID:Addgene_51695 Optopatch1 Synthetic Ampicillin PMID:24952910 Backbone Marker:Pavel Osten; Backbone Size:9236; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 2
pCDNA3-ySet2
 
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RRID:Addgene_51698 yeast Set2 Saccharomyces cerevisiae Ampicillin PMID:20133523 Backbone Marker:commercial; Backbone Size:5400; Vector Backbone:pcDNA3.1 +; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
R5 EMCV
 
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RRID:Addgene_51733 EMCV IRES Ampicillin PMID:22718970 Backbone Marker:Promega; Backbone Size:4600; Vector Backbone:pGl4.13; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 3
R5 MCS
 
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RRID:Addgene_51732 MCS Ampicillin PMID:22718970 Backbone Marker:Promega; Backbone Size:4600; Vector Backbone:pGl4.13; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:16:16 0
p413TEF-SirT1
 
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RRID:Addgene_51746 SirT1 Homo sapiens Ampicillin PMID:19390637 Vector Backbone:p413TEF; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:16:17 2

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