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  • Bacterial Resistance:kanamycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pET28a/Cas9-Cys
 
Resource Report
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10+ mentions
RRID:Addgene_53261 Cas9-Cys S. pyogenes Kanamycin PMID:24696462 A sequence encoding Cas9 with a cysteine at the C-terminus was prepared by PCR amplification using a previously described Cas9 plasmid (Cho et al, Nat Biotechnol. 2013)(Addgene: 43945) as the template and then cloned into the pET28-(a) vector (Novagen, Merk Millipore, Germany), which includes a sequence encoding a His-tag at the N-terminus. Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Additional Cysteine is added to the C-terminal region of Cas9 2026-08-15 01:16:29 23
pET28a(+) 6xHis-TEV-IFIT2
 
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1+ mentions
RRID:Addgene_53558 IFIT2 Homo sapiens Kanamycin PMID:23317505 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:33 1
pET28a(+) 6xHis-3xFlag-IFIT5
 
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1+ mentions
RRID:Addgene_53561 IFIT5 Homo sapiens Kanamycin PMID:23317505 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:33 1
pET28a(+) 6xHis-TEV-IFIT5
 
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1+ mentions
RRID:Addgene_53560 IFIT5 Homo sapiens Kanamycin PMID:23317505 Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:31 2
pEGFP-C1+SKL
 
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1+ mentions
RRID:Addgene_53450 SKL amino acids Synthetic Kanamycin PMID:21132080 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:33 3
NPC1 His6 EYFP
 
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1+ mentions
RRID:Addgene_53523 NPC1 Mus musculus Kanamycin PMID:21430138 Vector Backbone:N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:16:33 2
CUL3A-c013
 
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1+ mentions
RRID:Addgene_53672 CUL3A Other Kanamycin C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/4APF/ Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin solubilizing mutations I342R & L346D 2026-08-15 01:16:34 1
pMiR-RECK-3'UTR
 
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1+ mentions
RRID:Addgene_53614 RECK-3'UTR Homo sapiens Kanamycin PMID:22761427 Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin 2026-08-15 01:16:32 1
BRPF1B-c001
 
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1+ mentions
RRID:Addgene_53620 BRPF1B Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4LC2/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 3
GLS2A-c007
 
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RRID:Addgene_53621 GLS2A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4BQM/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
JMJD1BA-c068
 
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1+ mentions
RRID:Addgene_53627 JMJD1BA Other Kanamycin C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/4C8D/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 1
ETS2A-c023
 
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RRID:Addgene_53625 ETS2 Homo sapiens Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4MHV/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:34 0
STK3A-c042
 
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RRID:Addgene_53622 STK3A Homo sapiens Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4L0N/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:34 0
BAZ2AA-c002
 
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1+ mentions
RRID:Addgene_53623 BAZ2AA Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4LZ2/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 3
BLMA-c800
 
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RRID:Addgene_53631 BLMA Other Kanamycin N-terminal His6, Tev, C-terminal TEV, Biotin; both cleaved by TEV http://www.thesgc.org/structures/4CGZ/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC-NHT-CTB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:34 0
MYO5AA-c010
 
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RRID:Addgene_53632 MYO5AA Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4J5L/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
ERCC6A-c055
 
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RRID:Addgene_53637 ERCC6A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4CVO/ Use BL21(DE3)-R3-pRARE2 strain for expression. Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:34 0
ZBTB33A-c002
 
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RRID:Addgene_53651 ZBTB33A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/3FKC/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments. Mutation in 3' oligo sequence, E115A 2026-08-15 01:16:34 0
ZADH1A-c501
 
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RRID:Addgene_53650 ZADH1A Other Kanamycin C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/2VNA/ Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0
DYRK2A-c023
 
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RRID:Addgene_53659 DYRK2A Other Kanamycin T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4AZF/ Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin Bacterial expression for structure determination; may not be full ORF, see comments 2026-08-15 01:16:32 0

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