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Species: Lactococcus lactis
Genetic Insert: HrtR
Vector Backbone Description: Backbone Size:2216; Vector Backbone:PZE2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29798884
Comments: Blood sensor validated in murine and swine models.
The A17P mutation in ChuA is not expected to affect plasmid function.
Proper citation: RRID:Addgene_112533 Copy
Species: Photorhabdus luminescens
Genetic Insert: luxCDABE
Vector Backbone Description: Backbone Size:2216; Vector Backbone:PZE2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29798884
Comments: This plasmid contains tandem copies of the luciferase operon which are both functional.
Proper citation: RRID:Addgene_112530 Copy
Species: Lactococcus lactis
Genetic Insert: HrtR
Vector Backbone Description: Backbone Size:2216; Vector Backbone:PZE2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29798884
Proper citation: RRID:Addgene_112534 Copy
Species: Homo sapiens
Genetic Insert: miR-23b
Vector Backbone Description: Backbone Marker:Agilent; Vector Backbone:pShuttle; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21536891
Comments: clone into pShuttle-CMV using KpnI/NotI first
Proper citation: RRID:Addgene_112538 Copy
Genetic Insert: Integrase 9 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 9 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127533 Copy
Genetic Insert: Integrase 7 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pSB3K3; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 7 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127532 Copy
Genetic Insert: Integrase 5 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 5 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127531 Copy
Genetic Insert: Integrase 4 coding sequence codon optimized for A. thaliana expression.
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The Actin 2 promoter came from Aragao, F. J. L., et al. Plant Science 168, 1227-1233 (2005) and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991). The original integrase 4 coding sequence (before A. thaliana codon optimization) was obtained from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014).
Proper citation: RRID:Addgene_127530 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-R72K
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127683 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y66F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127681 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y63F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127680 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y98F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127686 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-Y76F
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127684 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6-P399A
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127689 Copy
Species: Synthetic
Genetic Insert: OphA-delta C6
Vector Backbone Description: Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30151425
Proper citation: RRID:Addgene_127679 Copy
Species: Homo sapiens
Genetic Insert: NONO sgRNA
Vector Backbone Description: Vector Backbone:gRNA_Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30270045
Proper citation: RRID:Addgene_127654 Copy
Species: Synthetic
Genetic Insert: RNA nanostrcutre
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29564885
Comments: The insert was designed by F. Chizzolini and M. Schwarz-Schilling and cloned by M.Schwarz-Schilling. The RNA sequence for the: 3WJ is from Shu, D. et al. Nucleic Acids Res. 2014, 42, e10; MG aptamer is from Babendure, J. R. et al. J. Am. Chem. Soc. 2003, 125, 14716−7; PP7 aptamer is from Chao, J. A. et al. Nat. Struct. Mol. Biol. 2008, 15, 103−5; TAR aptamer is from Bayer, T. S. et al. RNA 2005, 11, 1848−57; Streptavidin aptamer is from: Srisawat, C. and Engelke, D. R. RNA 2001, 7, 632−41.
Proper citation: RRID:Addgene_127634 Copy
Species: Homo sapiens
Genetic Insert: Tom70
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31894645
Proper citation: RRID:Addgene_127633 Copy
Species: Mus musculus
Genetic Insert: Trak1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24671417
Proper citation: RRID:Addgene_127621 Copy
Species: Homo sapiens
Genetic Insert: Miro1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31894645
Proper citation: RRID:Addgene_127628 Copy
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