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Species: S. pyogenes
Genetic Insert: Cas9-Cys
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24696462
Comments: A sequence encoding Cas9 with a cysteine at the C-terminus was prepared by PCR
amplification using a previously described Cas9 plasmid (Cho et al, Nat Biotechnol. 2013)(Addgene: 43945) as the template and then cloned into the pET28-(a) vector (Novagen, Merk Millipore, Germany), which
includes a sequence encoding a His-tag at the N-terminus.
Proper citation: RRID:Addgene_53261 Copy
Species: Homo sapiens
Genetic Insert: IFIT2
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23317505
Proper citation: RRID:Addgene_53558 Copy
Species: Homo sapiens
Genetic Insert: IFIT5
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23317505
Proper citation: RRID:Addgene_53561 Copy
Species: Homo sapiens
Genetic Insert: IFIT5
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23317505
Proper citation: RRID:Addgene_53560 Copy
Species: Synthetic
Genetic Insert: SKL amino acids
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21132080
Proper citation: RRID:Addgene_53450 Copy
Species: Mus musculus
Genetic Insert: NPC1
Vector Backbone Description: Vector Backbone:N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21430138
Proper citation: RRID:Addgene_53523 Copy
Species: Other
Genetic Insert: CUL3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/4APF/
Proper citation: RRID:Addgene_53672 Copy
Species: Homo sapiens
Genetic Insert: RECK-3'UTR
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22761427
Proper citation: RRID:Addgene_53614 Copy
Species: Other
Genetic Insert: BRPF1B
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4LC2/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53620 Copy
Species: Other
Genetic Insert: GLS2A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4BQM/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53621 Copy
Species: Other
Genetic Insert: JMJD1BA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/4C8D/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53627 Copy
Species: Homo sapiens
Genetic Insert: ETS2
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4MHV/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53625 Copy
Species: Homo sapiens
Genetic Insert: STK3A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4L0N/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53622 Copy
Species: Other
Genetic Insert: BAZ2AA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4LZ2/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53623 Copy
Species: Other
Genetic Insert: BLMA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-NHT-CTB; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: N-terminal His6, Tev, C-terminal TEV, Biotin; both cleaved by TEV http://www.thesgc.org/structures/4CGZ/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53631 Copy
Species: Other
Genetic Insert: MYO5AA
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4J5L/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53632 Copy
Species: Other
Genetic Insert: ERCC6A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4CVO/ Use BL21(DE3)-R3-pRARE2 strain for expression.
Proper citation: RRID:Addgene_53637 Copy
Species: Other
Genetic Insert: ZBTB33A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/3FKC/
Proper citation: RRID:Addgene_53651 Copy
Species: Other
Genetic Insert: ZADH1A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC-CTHF; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: C-terminal His+flag, TEV-cleavable, for LIC cloning. EXP-06-AA9343 http://www.thesgc.org/structures/2VNA/
Proper citation: RRID:Addgene_53650 Copy
Species: Other
Genetic Insert: DYRK2A
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4AZF/
Proper citation: RRID:Addgene_53659 Copy
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