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Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pmTurquoise2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Comments: Please note that there is a 3bp mismatch between Addgene's quality control sequence and the provided sequence from the depositing lab. This mismatch should not affect function or localization.
Proper citation: RRID:Addgene_36207 Copy
Species: Homo sapiens
Genetic Insert: Golgi targeting sequence
Vector Backbone Description: Vector Backbone:pmTurquoise2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Proper citation: RRID:Addgene_36205 Copy
Species: Homo sapiens
Genetic Insert: Sec23A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLVX-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21045114
Comments: The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage
adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II
SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech)
to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplified
by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI)
and cloned into pLVX-Puro–mRuby.
Proper citation: RRID:Addgene_36158 Copy
Genetic Insert: HE-6
Vector Backbone Description: Vector Backbone:pBAD33.1; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36279 Copy
Species: Homo sapiens
Genetic Insert: p150Glued
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16772339
Proper citation: RRID:Addgene_36154 Copy
Species: Rattus norvegicus
Genetic Insert: CD200R
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_36153 Copy
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20394418
Proper citation: RRID:Addgene_36267 Copy
Species: Mus musculus
Genetic Insert: Dynamin 3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17463283
Proper citation: RRID:Addgene_36265 Copy
Species: Mus musculus
Genetic Insert: Dynamin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17463283
Comments: HA-tagged mouse dynamin 2 flanked by KpnI and XbaI sites. BamHI between N-terminal HA tag and beginning of dynamin coding seq.
Proper citation: RRID:Addgene_36264 Copy
Species: Homo sapiens
Genetic Insert: MBD3
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36371 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-1 (NOT pEGFP-N1); Vector Types:Fluorescent protein cassette for sub-cloning; Bacterial Resistance:Kanamycin
Comments: Plasmid carrying monomeric (A206K) EGFP for C-terminal tagging
Proper citation: RRID:Addgene_36409 Copy
Species: Homo sapiens
Genetic Insert: YY1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36358 Copy
Species: Homo sapiens
Genetic Insert: Suv39H2
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36344 Copy
Species: Homo sapiens
Genetic Insert: Dot1L
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36339 Copy
Species: Homo sapiens
Genetic Insert: Ehmt1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36338 Copy
Vector Backbone Description: Backbone Size:5678; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20671728
Proper citation: RRID:Addgene_36295 Copy
Genetic Insert: M13-DDRFP-B1
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22444590
Proper citation: RRID:Addgene_36293 Copy
Genetic Insert: ddRFPA1-CaM
Vector Backbone Description: Backbone Marker:Life Technologies; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22444590
Proper citation: RRID:Addgene_36292 Copy
Species: Homo sapiens
Genetic Insert: shRNA of beta-catenin-1
Vector Backbone Description: Backbone Marker:Biosettia; Backbone Size:10275; Vector Backbone:pLV-H1TetO-RFP-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Proper citation: RRID:Addgene_36297 Copy
Species: Synthetic
Genetic Insert: 4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and circularly permuted Venus 173. D3cpv does not bind wild-type calmodulin. It has a Kd' of 0.6 uM.
See also Palmer & Tsien (2006) Nature Protocols.
The small discrepancy between the Addgene and depositor's sequence should not alter the function of the plasmid.
Proper citation: RRID:Addgene_36324 Copy
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