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pCI-neo-RL-ZEB2 200bmut
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_35538 3'UTR of ZEB2 gene Homo sapiens Ampicillin PMID:18376396 Mutations in the miR-200b seed regions of the ZEB2 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit. The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz. Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells. Science 309:1573-1576 Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact. Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin 6 Mutations in the miR-200b seed regions of the ZEB2 3'UTR 2026-08-15 01:14:02 1
pcDNA3/Myc-DNMT3L
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_35523 DNMT3L Homo sapiens Ampicillin PMID:15861382 The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI and BamHI restriction sites (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917. The full-length cDNA for human DNMT3L was cloned into EcoRI and XhoI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:01 2
pGEX 4T1 ERbeta(144-530)
 
Resource Report
Resource Website 		RRID:Addgene_35566 Estrogen Receptor beta Homo sapiens Ampicillin PMID:20660297 Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin residues 144-530 2026-08-15 01:14:02 0
pCMV-mCherry-MHC-IIA
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_35687 MHC IIA Homo sapiens Kanamycin PMID:17567956 Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:07 11
pGEX 4T1 ERbeta(144-255)
 
Resource Report
Resource Website 		RRID:Addgene_35565 Estrogen Receptor beta Homo sapiens Ampicillin PMID:20660297 Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin residues 144-255 2026-08-15 01:14:06 0
pGEX 4T1 ERbeta(1-255)
 
Resource Report
Resource Website 		RRID:Addgene_35564 Estrogen Receptor beta Homo sapiens Ampicillin PMID:20660297 Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin residues 1-255 2026-08-15 01:14:02 0
pGEX 4T1 hBad (1-146)
 
Resource Report
Resource Website 		RRID:Addgene_35569 bad Homo sapiens Ampicillin PMID:20660297 A G21C point mutation is present in the sequence. The depositing lab notes that this should not alter the function of the plasmid. Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin residues 1-146 2026-08-15 01:14:02 0
pGEX 4T1 hBad(1-101)
 
Resource Report
Resource Website 		RRID:Addgene_35568 bad Homo sapiens Ampicillin PMID:20660297 Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin residues 1-101 2026-08-15 01:14:06 0
pCMV-eGFP-NMHC-IIAΔtailpiece
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_35689 MHC IIA Homo sapiens Kanamycin PMID:18971378 Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Added stop codon after codon 1928, eliminating the last 33 amino acids (corresponding to the non-helical tailpiece) 2026-08-15 01:14:03 2
pcDNA Flag ERbeta
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_35562 Estrogen Receptor beta Homo sapiens Ampicillin PMID:20660297 Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:06 12
pTWEEN-miR-224
 
Resource Report
Resource Website 		RRID:Addgene_35683 nsa-mir-224 Homo sapiens Ampicillin Note that though there is GFP in this plasmid, it is not fused to the miR-224. There is a PGK promoter between the miR and the GFP. Backbone Marker:provided by PESCHLE C. (PMID:163300772) - CONDORELLI G (PMID: 12692591); Backbone Size:8600; Vector Backbone:pRRL-CMV-PGK-GFP-WPRE (TWEEN); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:03 0
pSUPER ERbeta shRNA
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_35561 ERbeta Homo sapiens Ampicillin PMID:19106194 Vector Backbone:pSUPER puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:02 2
pEGFP-MRLC T18A,S19A
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_35681 MRLC1 Homo sapiens Kanamycin PMID:22136066 This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T18A and S19A 2026-08-15 01:14:07 5
pEGFP-MRLC1
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_35680 MRLC1 Homo sapiens Kanamycin PMID:22136066 This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:14:03 21
pLenti-PGK-NRAS(G12V)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_35632 N-Ras(G12V) Homo sapiens Ampicillin PMID:22341439 Backbone Marker:Eric Campeau; Backbone Size:10000; Vector Backbone:pLenti-PGK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Glycine 12 to Valine 2026-08-15 01:14:02 3
pLenti-PGK-KRAS4A(G12V)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_35634 K-Ras4A(G12V) Homo sapiens Ampicillin PMID:22341439 Backbone Marker:Eric Campeau; Backbone Size:10000; Vector Backbone:pLenti-PGK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Glycine 12 to Valine 2026-08-15 01:14:02 2
pLenti-PGK-KRAS4B(G12V)
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_35633 K-Ras4B(G12V) Homo sapiens Ampicillin PMID:22341439 Backbone Marker:Eric Campeau; Backbone Size:10000; Vector Backbone:pLenti-PGK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Glycine 12 to Valine 2026-08-15 01:14:07 12
pLenti6-BMPR1A(Q233D)-V5
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_35638 BMP Receptor Type1A Q233D Homo sapiens Ampicillin PMID:22341439 Backbone Marker:Invitrogen; Backbone Size:10000; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Glutamine 233 to Aspartate 2026-08-15 01:14:02 1
pCMV-eGFP-NMHC-IIAdeltaIQ2
 
Resource Report
Resource Website 		RRID:Addgene_35690 MHC IIA Homo sapiens Kanamycin PMID:18971378 Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin The coding segment for aa 804-834 (corresponding to the IQ2 motif that binds RLC) was deleted with the deletion junction marked by an engineered NheI restriction site. 2026-08-15 01:14:07 0
pEGFP-N1-human lyn(C468A)–GFP
 
Resource Report
Resource Website 		RRID:Addgene_35959 LYN Homo sapiens Kanamycin PMID:22101434 Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin c468a 2026-08-15 01:14:07 0

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