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Species: Homo sapiens
Genetic Insert: 3'UTR of ZEB2 gene
Vector Backbone Description: Backbone Marker:Witold Filipowicz; Backbone Size:6380; Vector Backbone:pCI-neo-RL; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18376396
Comments: Mutations in the miR-200b seed regions of the ZEB2 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit.
The ZEB2 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.
Pillai R, Bhattacharyya S, Artus C, Zoller T, Cougot N, Basyuk E, Bertrand E, Filipowicz W (2005) Inhibition of translational initiation by let-7 microRNA in human cells.
Science 309:1573-1576
Please note that Addgene's sequencing results identified a single nucleotide deletion at bp# 2440 when compared to the full plasmid sequence provided by the depositing laboratory. Addgene's sequencing results are correct as the extra base in the sequence from the depositing laboratory was an error due to a sequencing artifact.
Proper citation: RRID:Addgene_35538 Copy
Species: Homo sapiens
Genetic Insert: DNMT3L
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15861382
Comments: The multiple cloning region of the pcDNA3 vector has been modified. In the modified vector, referred to as pcDNA3/Myc, the region of the multiple cloning site located between the HindIII and NotI sites was replaced by a DNA segment containing a Kozak consensus sequence, an ATG start site and the Myc epitope sequence followed by EcoRI and BamHI restriction sites (see supplemental document). The construction of pcDNA3/Myc has been described in Chen, Z.X., et al. J Cell Biochem, 95: 902-917.
The full-length cDNA for human DNMT3L was cloned into EcoRI and XhoI sites of pcDNA3/Myc. Constructed by Zhaoxia Chen
Proper citation: RRID:Addgene_35523 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35566 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17567956
Proper citation: RRID:Addgene_35687 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35565 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35564 Copy
Species: Homo sapiens
Genetic Insert: bad
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Comments: A G21C point mutation is present in the sequence. The depositing lab notes that this should not alter the function of the plasmid.
Proper citation: RRID:Addgene_35569 Copy
Species: Homo sapiens
Genetic Insert: bad
Vector Backbone Description: Vector Backbone:pGEX 4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35568 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18971378
Proper citation: RRID:Addgene_35689 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35562 Copy
Species: Homo sapiens
Genetic Insert: nsa-mir-224
Vector Backbone Description: Backbone Marker:provided by PESCHLE C. (PMID:163300772) - CONDORELLI G (PMID: 12692591); Backbone Size:8600; Vector Backbone:pRRL-CMV-PGK-GFP-WPRE (TWEEN); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Note that though there is GFP in this plasmid, it is not fused to the miR-224. There is a PGK promoter between the miR and the GFP.
Proper citation: RRID:Addgene_35683 Copy
Species: Homo sapiens
Genetic Insert: ERbeta
Vector Backbone Description: Vector Backbone:pSUPER puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19106194
Proper citation: RRID:Addgene_35561 Copy
Species: Homo sapiens
Genetic Insert: MRLC1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22136066
Comments: This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
Proper citation: RRID:Addgene_35681 Copy
Species: Homo sapiens
Genetic Insert: MRLC1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22136066
Comments: This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
Proper citation: RRID:Addgene_35680 Copy
Species: Homo sapiens
Genetic Insert: N-Ras(G12V)
Vector Backbone Description: Backbone Marker:Eric Campeau; Backbone Size:10000; Vector Backbone:pLenti-PGK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22341439
Proper citation: RRID:Addgene_35632 Copy
Species: Homo sapiens
Genetic Insert: K-Ras4A(G12V)
Vector Backbone Description: Backbone Marker:Eric Campeau; Backbone Size:10000; Vector Backbone:pLenti-PGK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22341439
Proper citation: RRID:Addgene_35634 Copy
Species: Homo sapiens
Genetic Insert: K-Ras4B(G12V)
Vector Backbone Description: Backbone Marker:Eric Campeau; Backbone Size:10000; Vector Backbone:pLenti-PGK-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22341439
Proper citation: RRID:Addgene_35633 Copy
Species: Homo sapiens
Genetic Insert: BMP Receptor Type1A Q233D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10000; Vector Backbone:pLenti6; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22341439
Proper citation: RRID:Addgene_35638 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18971378
Proper citation: RRID:Addgene_35690 Copy
Species: Homo sapiens
Genetic Insert: LYN
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22101434
Proper citation: RRID:Addgene_35959 Copy
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