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Species: Synthetic
Genetic Insert: mVenusNB-MrkHR113A
Vector Backbone Description: Backbone Marker:J. H. Davis, A. J. Rubin, R. T. Sauer, Design, construction and characterization of a set of insulated bacterial promoters.; Backbone Size:3200; Vector Backbone:pEB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35131853
Comments: We obtained the codon optimized MrkH sequence from Maria Schumacher, as described in her publication PMID: 27551088
Proper citation: RRID:Addgene_182292 Copy
Species: Brevibacillus sp. SYP-B805
Genetic Insert: BrCas12b
Vector Backbone Description: Backbone Marker:Scott Gradia Lab; Backbone Size:6462; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35290826
Proper citation: RRID:Addgene_182276 Copy
Species: Synthetic
Genetic Insert: GFP 1-9 OPT
Vector Backbone Description: Backbone Marker:pEGFP (Clontech); Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24092409
Proper citation: RRID:Addgene_182244 Copy
Genetic Insert: mCherry-Fis1
Vector Backbone Description: Vector Backbone:pmCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32778832
Proper citation: RRID:Addgene_182580 Copy
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Primers for LIC cloning: Upstream: add TATTTTCAATCCTACGTA to the 5’ end of gene of interest. Downstream: add CCCTCAATATTATACGGG to the 3’ end of GOI. Detailed cloning method available in the paper Bruni R, Kloss B. High-throughput cloning and expression of integral membrane proteins in Escherichia coli. Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34.
Proper citation: RRID:Addgene_182506 Copy
Species: Mus musculus
Genetic Insert: glyceraldehyde-3-phosphate dehydrogenase
Vector Backbone Description: Backbone Size:3937; Vector Backbone:pDendra2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34996455
Proper citation: RRID:Addgene_182368 Copy
Species: Homo sapiens
Genetic Insert: Rap2b
Vector Backbone Description: Backbone Marker:clontech; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20547757
Comments: human Rap2b was purchased from the Missouri S&T cDNA resouce center
Proper citation: RRID:Addgene_118320 Copy
Species: Bos taurus
Genetic Insert: Rap1b E63
Vector Backbone Description: Backbone Marker:clontech; Vector Backbone:peGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23946483
Proper citation: RRID:Addgene_118328 Copy
Species: Other
Genetic Insert: MvuTFIIB intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118397 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118399 Copy
Species: Other
Genetic Insert: MjaTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118393 Copy
Species: Other
Genetic Insert: MjaTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118395 Copy
Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118379 Copy
Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118378 Copy
Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118383 Copy
Species: Homo sapiens
Genetic Insert: actin beta
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Vector Backbone:pENTR-TOPO; Vector Types:Donor-plasmid; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30890647
Comments: Please visit https://www.biorxiv.org/content/10.1101/445973v1 for BioRxiv preprint
Proper citation: RRID:Addgene_118380 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118401 Copy
Species: Other
Genetic Insert: MvuTFIIB mini intein
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29055778
Proper citation: RRID:Addgene_118400 Copy
Species: Homo sapiens
Genetic Insert: hSUMO1
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4735; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12665592
Proper citation: RRID:Addgene_118360 Copy
Species: Synthetic
Genetic Insert: mClover3
Vector Backbone Description: Vector Backbone:pFru97; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30631309
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/07/402172 for bioRxiv preprint.
Proper citation: RRID:Addgene_118499 Copy
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