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Species: HPV
Genetic Insert: HPV 16E6no*
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9387; Vector Backbone:pLenti 6.3/V5 DEST; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Proper citation: RRID:Addgene_37445 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Backbone Marker:Eric Campeau Addgene Plasmid 17448; Backbone Size:8608; Vector Backbone:pLentiCMV GFP Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37561 Copy
Species: Homo sapiens
Genetic Insert: MET
Vector Backbone Description: Backbone Marker:Eric Campeau Addgene Plasmid 17448; Backbone Size:8608; Vector Backbone:pLentiCMV GFP Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37560 Copy
Species: Homo sapiens
Genetic Insert: Huwe1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2717; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: Full-length cDNA constructed by sequential cloning of PCR products and adding to a partial cDNA.
The ORF sequence provided contains Q2315H and 3016del3031 amino acid mutations compared to closest reference sequence NP_113584.3).
Proper citation: RRID:Addgene_37432 Copy
Species: Homo sapiens
Genetic Insert: IRF-9 STAT2 C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12574168
Comments: C8Y point mutation in IRF9 should not affect plasmid function.
Proper citation: RRID:Addgene_37544 Copy
Species: Homo sapiens
Genetic Insert: Rabenosyn5
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22308388
Comments: Designed and prepared by Deborah Leonard, Deanna Navaroli and Anil Chawla (Corvera Lab)
Proper citation: RRID:Addgene_37538 Copy
Species: Homo sapiens
Genetic Insert: AK3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5597; Vector Backbone:pcDNA3.1(+) hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21698293
Proper citation: RRID:Addgene_37539 Copy
Species: Homo sapiens
Genetic Insert: cysteine-free SGFP2-N
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4105; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22649538
Proper citation: RRID:Addgene_37533 Copy
Species: Homo sapiens
Genetic Insert: ss-cfSGFP2 (cysteine-free SGFP2)
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4105; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22649538
Comments: This plasmid is used for expression of cfSGFP2 in the ER. The cleaved signal sequence of α1-antitrypsin (M1-K34) was fused to the N-terminus of SGFP2 using BglII and EcoRI sites. The fragment M1-A24 should be removed cotranslationally after translocation. The long flanking sequence E25-K34 of α1-antitrypsin was included to ensure access to signal peptidase
Proper citation: RRID:Addgene_37535 Copy
Species: Homo sapiens
Genetic Insert: NLRC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22490867
Comments: The iso3 plasmid actually encodes the full cDNA (not only the ORF).
In comparison to the FL NLRC5, iso3 contains an insert that leads to a stop codon according to IPI00647281.1 .
Proper citation: RRID:Addgene_37510 Copy
Vector Backbone Description: Backbone Marker:Amaxa; Backbone Size:2800; Vector Backbone:pmaxGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:18245377
Proper citation: RRID:Addgene_37631 Copy
Species: Synthetic
Genetic Insert: D4 second generation cameleon (calcium sensor)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and citrine. D4 does not bind wild-type calmodulin. It has a Kd' of 195 microM.
See also Palmer & Tsien (2006) Nature Protocols.
Proper citation: RRID:Addgene_37473 Copy
Species: Drosophila melanogaster
Genetic Insert: Rab4
Vector Backbone Description: Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17409086
Proper citation: RRID:Addgene_37691 Copy
Species: Mus musculus
Genetic Insert: shh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.
Proper citation: RRID:Addgene_37680 Copy
Species: Mus musculus
Genetic Insert: shh
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12372301
Comments: The ShhN construct contains a stop codon following the site of internal cleavage and therefore produces a protein (ShhN) containing the same amino acid residues as the processed protein but lacking cholesterol modification.
Renilla luciferase was inserted in-frame into the amino-terminal signaling domain located in a peripheral loop within the structure of ShhN.
Proper citation: RRID:Addgene_37677 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19996169
Proper citation: RRID:Addgene_37671 Copy
Species: Mus musculus
Genetic Insert: Gli2
Vector Backbone Description: Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19996169
Comments: monomeric GFP was constructed by introducing the monomeric mutation, A206K, to EGFP.
Proper citation: RRID:Addgene_37672 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Vector Backbone:pEG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10984056
Comments: Addgene NGS results identified a D54N variant compared to the NCBI reference [EDL13766.1].
Proper citation: RRID:Addgene_37674 Copy
Species: Homo sapiens
Genetic Insert: NUMB isoform 2
Vector Backbone Description: Backbone Marker:Beyer et al., J Virol 2002; Backbone Size:7309; Vector Backbone:M107; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21939656
Proper citation: RRID:Addgene_37803 Copy
Species: H. strain TP009
Genetic Insert: ArchT-GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5088; Vector Backbone:AAV with CaMKII promoter; Vector Types:Mammalian Expression, AAV, Adeno-associated virus; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Comments: PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER REAGENTS IN THIS LINE.
Proper citation: RRID:Addgene_37807 Copy
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