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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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Duet011 Resource Report Resource Website 1+ mentions |
RRID:Addgene_17627 | UBC promoter - GFP - PGK promoter - Hygro | Ampicillin | PMID:17158240 | Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene). | Backbone Size:0; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 4 | ||
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pMVS223 Resource Report Resource Website 1+ mentions |
RRID:Addgene_176293 | Ampicillin | PMID:35120642 | Please visit https://www.biorxiv.org/content/10.1101/2020.10.28.359026v2 for bioRxiv preprint. | Backbone Size:5897; Vector Backbone:pMVS223; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 2 | |||
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pAB1678 CMV-HIVNES-GS-Cas13bt1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_176316 | human codon optimized Cas13bt1 | Atlantic deep subsurface metagenome | Ampicillin | PMID:34462587 | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 1 | ||
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pGST1-Osh4 (2-434) Resource Report Resource Website 1+ mentions |
RRID:Addgene_17632 | Osh4 | Saccharomyces cerevisiae | Ampicillin | PMID:16136145 | DNA encoding Osh4 residues 2–434 was amplified by PCR and subcloned into the BamHI and XhoI sites of a pGEX-4T vector modified to contain a TEV protease recognition site. | Backbone Size:5000; Vector Backbone:modified pGEX-4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:57 | 1 | |
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pGFP-Ub-VPS13D Resource Report Resource Website 1+ mentions |
RRID:Addgene_176462 | VPS13D | Homo sapiens | Kanamycin | PMID:33891012 | GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression. | Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:59 | 1 | |
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GFP-HP1beta Resource Report Resource Website 1+ mentions |
RRID:Addgene_17651 | HP1 beta | Homo sapiens | Ampicillin | PMID:12560555 | See author's map for more information. | Backbone Marker:Zentgraf Lab; Backbone Size:0; Vector Backbone:pBCHGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:59 | 8 | |
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GFP-HP1alpha Resource Report Resource Website 10+ mentions |
RRID:Addgene_17652 | HP1 alpha | Homo sapiens | Ampicillin | PMID:12560555 | See author's map for more information. | Backbone Marker:Zentgraf Lab; Backbone Size:0; Vector Backbone:pBCHGN; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:59 | 20 | |
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pEGFP-D50 lamin A Resource Report Resource Website 10+ mentions |
RRID:Addgene_17653 | D50 lamin A | Homo sapiens | Kanamycin | PMID:15750600 | Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Delta 50 (50 aa internal deletion in globular tail domain) | 2026-08-15 01:10:59 | 14 | |
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pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep Resource Report Resource Website 1+ mentions |
RRID:Addgene_176534 | CRISPR-associated IscB (Delaware Bay metagenome) | E. coli, Brachypodium distachyon, Delaware Bay metagenome | Ampicillin | PMID:34591643 | Backbone Size:5062; Vector Backbone:pET45b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:00 | 2 | ||
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pBABE-hygro Resource Report Resource Website 10+ mentions |
RRID:Addgene_1765 | Ampicillin | PMID:2194165 | Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96. If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC. Please note that the sequence from which the Addgene map was generated is an estimate of the real sequence based on how the vector was assembled. We encourage scientists to test enzymes before using them for cloning. | Backbone Size:5558; Vector Backbone:pBABE-hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:59 | 36 | |||
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GFP-Trim46 Resource Report Resource Website 1+ mentions |
RRID:Addgene_176402 | trim46 | Mus musculus | Ampicillin | PMID:26671463 | Vector Backbone:?pGW1-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:58 | 2 | ||
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pGST2-Alix (360-702) Resource Report Resource Website 1+ mentions |
RRID:Addgene_17639 | Alix (360-702) | Homo sapiens | Ampicillin | PMID:17277784 | The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467). | Backbone Size:5000; Vector Backbone:parallel GST2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Alix V domain | 2026-08-15 01:10:58 | 1 |
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pcDNA3-myc-his-BACH1 K52R Resource Report Resource Website 1+ mentions |
RRID:Addgene_17643 | BACH1 | Homo sapiens | Ampicillin | PMID:14983014 | Backbone Marker:Invitrogen; Backbone Size:5493; Vector Backbone:pcDNA3.1(+)/myc-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | The lysine residue at position 52 was changed to an arginine, using a Quik-change site-directed mutagenesis kit (Stratagene). | 2026-08-15 01:10:58 | 2 | |
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pCMV-Tag1-NS5A Resource Report Resource Website 1+ mentions |
RRID:Addgene_17646 | HCV NS5A | Hepatitis C virus | Kanamycin | PMID:17404395 | Backbone Marker:Stratagene; Backbone Size:4300; Vector Backbone:pCMVTag1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:10:59 | 4 | ||
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Flag-hCRM1 Resource Report Resource Website 10+ mentions |
RRID:Addgene_17647 | CRM1 | Homo sapiens | Ampicillin | PMID:16041368 | Backbone Marker:Sigma; Backbone Size:6400; Vector Backbone:p3xFLAG CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:10:59 | 14 | ||
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pCS2-MT GLI2 delta N Resource Report Resource Website 10+ mentions |
RRID:Addgene_17649 | GLI2 | Homo sapiens | Ampicillin | PMID:15994174 | Backbone Size:4350; Vector Backbone:pCS2-MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Missing 328 N-terminal amino acids | 2026-08-15 01:10:59 | 12 | |
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pET29b-mVenus-dspB(E184Q W330Y)-6xHis Resource Report Resource Website 1+ mentions |
RRID:Addgene_176577 | Dispersin B | Aggregatibacter actinomycetemcomitans | Kanamycin | PMID:35379435 | Backbone Size:5313; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | E184Q W330Y | 2026-08-15 01:11:00 | 1 | |
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BL21 Rosetta2 ΔrecBCD Resource Report Resource Website 1+ mentions |
RRID:Addgene_176583 | pRARE2 plasmid | Chloramphenicol | PMID:35034449 | Please visit https://www.biorxiv.org/content/10.1101/2021.09.07.459228v1 for bioRxiv preprint. Primers for recBCD deletion verification: Foward - ttgatttactgcccgagagc Reverse - gtcaaccgaatgcagacatc | Vector Backbone:Strain; Vector Types:; Bacterial Resistance:Chloramphenicol | recBCD genomic deletion | 2026-08-15 01:11:00 | 1 | |
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pCDH-EF1-mVenus-p27K− Resource Report Resource Website 1+ mentions |
RRID:Addgene_176651 | mVenus-p27K- | Mus musculus | Ampicillin | PMID:34079127 | Backbone Marker:System Biosciences; Backbone Size:7082; Vector Backbone:pCDH; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | Mutant K- (lacks affinity to Cdk) | 2026-08-15 01:11:00 | 5 | |
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Lac-I-SceI-Tet Resource Report Resource Website 1+ mentions |
RRID:Addgene_17655 | lac I-SceI tet | Ampicillin | PMID:17486118 | The 256 lac operator repeats (XhoI fragment) and the 96 tet operator repeats (XhoI –SacII fragment) from the p16PCbeta plasmid (gift from D. Spector) were cloned into pBluescript. Between the two fragments a single 18nt I-SceI site was inserted (SalI). lac operator repeat: tggaattgtgagcggataacaatt tet operator repeat: tccctatcagtgatagaga This plasmid is not stable in bacteria. Every bacterial stock made will contain correct and incorrect bacteria and when bacteria are grown, you will get colonies which contain the correct plasmid and others which contain recombinant versions. If you check multiple colonies (10-20) in the samples after streaking out a stab, you should find correct ones. The recipient will need to grow the bacteria, isolate individual colonies check them, and make a prep from the correct one. We recommend processing the stab and screening colonies immediately upon receipt. | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:00 | 4 |
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