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Species: Homo sapiens
Genetic Insert: UBF
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12446911
Proper citation: RRID:Addgene_17656 Copy
Species: Mus musculus
Genetic Insert: RPA194
Vector Backbone Description: Backbone Size:4730; Vector Backbone:modified pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12446911
Proper citation: RRID:Addgene_17660 Copy
Species: Homo sapiens
Genetic Insert: RRN3/TIF1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12446911
Comments: Please note: This plasmid contains a F236L mutation in RRN3 compared to the NCBI reference sequence. It is not known how this mutation affects plasmid function.
Proper citation: RRID:Addgene_17661 Copy
Species: Homo sapiens
Genetic Insert: GFP-lamin A
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18311132
Comments: This vector was generated by cloning the NheI–BamHI fragment from pEGFP–wt-lamin A into the BamHI site of the pBABE-puro vector after filling of the ends.
Proper citation: RRID:Addgene_17662 Copy
Species: Homo sapiens
Genetic Insert: GFP-D50 lamin A
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18311132
Comments: This vector was generated by cloning the NheI–BamHI fragment from pEGFP–D50 lamin A into the BamHI site of the pBABE-puro vector after filling of the ends.
This plasmid exists as a dimer in our stock. Dimerziation often does not impact plasmid function, but may reduce transformation efficiencies. If you need the monomeric version, you could try linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_17663 Copy
Vector Backbone Description: Backbone Size:4888; Vector Backbone:pBABE-zeo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2194165
Comments: Please acknowledge Jay Morgenstern and Hartmut Land and cite the following article if you use this plasmid in a publication:
Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.
SspI site in Amp gene.
If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that the Weinberg Lab recommends using pUMVC (Addgene #8449) and VSV-G (#8454) for packaging. The pCL-Eco plasmid listed in their protocol should be substituted with pUMVC.
Proper citation: RRID:Addgene_1766 Copy
Species: Mus musculus
Genetic Insert: Cx43 CT
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:3800; Vector Backbone:p3XFLAG-CMV-7.1?; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_17664 Copy
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34819517
Comments: Cloning of sgRNA targeting sequence into BbsI cassette.
Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437496v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_176668 Copy
Species: Homo sapiens
Genetic Insert: Cas12f-GE ver4.1
Vector Backbone Description: Vector Backbone:px458-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34475560
Proper citation: RRID:Addgene_176544 Copy
Species: Homo sapiens
Genetic Insert: hYAP1-5SA
Vector Backbone Description: Backbone Marker:VectorBuilder; Backbone Size:8562; Vector Backbone:pLV[Tet]-mCherry:T2A:Puro-TRE3G; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Proper citation: RRID:Addgene_176851 Copy
Species: Homo sapiens
Genetic Insert: hPLXNB2
Vector Backbone Description: Backbone Marker:Eric Campeau (Addgene plasmid # 26432); Backbone Size:9185; Vector Backbone:pLenti CMVtight Neo DEST (w770-1); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34650052
Proper citation: RRID:Addgene_176849 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP8f
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176756 Copy
Species: Mus musculus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Size:0; Vector Backbone:pcDNA3.1/CT-GFP-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Amino acids 234-243 deleted (tubulin binding site).
Proper citation: RRID:Addgene_17671 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP8m
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176751 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP8f
Vector Backbone Description: Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176750 Copy
Species: Gallus gallus
Genetic Insert: c-src K295R
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pEVX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1702522
Comments: Based on plasmid pM5HHB5.
Proper citation: RRID:Addgene_17678 Copy
Species: Mus musculus
Genetic Insert: c-src
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pEVX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7530829
Proper citation: RRID:Addgene_17685 Copy
Species: Mus musculus
Genetic Insert: c-src Y529F
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pEVX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7530829
Proper citation: RRID:Addgene_17686 Copy
Species: Rattus norvegicus
Genetic Insert: lck-jGCaMP8s
Vector Backbone Description: Vector Backbone:pZac2.1; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_176761 Copy
Genetic Insert: Cre-P2A-tdTomato
Vector Backbone Description: Vector Backbone:AAVS1-tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35452598
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.03.24.485641v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176837 Copy
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