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Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.
Proper citation: RRID:Addgene_37855 Copy
Species: Simian Virus 40
Genetic Insert: SV40 ST
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37858 Copy
Species: codon-modified firefly (Photinus pyralis)
Genetic Insert: 3 copies of ERRE upstream of luciferase
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5010; Vector Backbone:pGL3-Promoter; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24684682
Proper citation: RRID:Addgene_37851 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:E. coli bacterial strain; Bacterial Resistance:None
Defining Citation: PMID:21110891
Proper citation: RRID:Addgene_37854 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17086198
Proper citation: RRID:Addgene_37843 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: BARF1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Though there are some discrepancies between the depositor sequence and Addgene's quality control sequence, they are not known to affect plasmid function.
Proper citation: RRID:Addgene_37922 Copy
Species: HPV
Genetic Insert: HPV 18E6
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37884 Copy
Species: HPV
Genetic Insert: HPV 18 E7
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-C FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37886 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #3
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37927 Copy
Species: Homo sapiens
Genetic Insert: EB1 shRNA #1
Vector Backbone Description: Backbone Marker:Addgene plasmid 8453; Backbone Size:7032; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37928 Copy
Species: Homo sapiens
Genetic Insert: Nucleoporin 98
Vector Backbone Description: Backbone Size:2968; Vector Backbone:pPEP-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21685911
Comments: see also:
Strelkov, S. V., Kreplak, L., Herrmann, H. & Aebi, U. (2004). Methods Cell Biol.78, 25–43.
Proper citation: RRID:Addgene_38037 Copy
Species: Homo sapiens
Genetic Insert: E2F3a
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8246996
Comments: E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.
The 5' end of the coding sequence can be difficult to sequence.
Proper citation: RRID:Addgene_37970 Copy
Species: Homo sapiens
Genetic Insert: E2F6
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11171983
Comments: The backbone plasmid pCMV-Neo-Bam is Addgene#16440
E2Fs bind DNA as a heterodimer with members of the DP family of transcription factors.
Proper citation: RRID:Addgene_37965 Copy
Species: Homo sapiens
Genetic Insert: DP1
Vector Backbone Description: Backbone Marker:Vogelstein Lab ; Backbone Size:6600; Vector Backbone:pCMV-Neo-Bam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8657117
Comments: DP1 functions as a heterodimer with several members of the E2F family of transcription factors.
Proper citation: RRID:Addgene_37968 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: LMP1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Proper citation: RRID:Addgene_37962 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: LMP2A
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [FLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37963 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38012 Copy
Species: H. Herpesvirus 4 (EBV)
Genetic Insert: EBNA1
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22810586
Comments: Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, leaving additional amino acid residues [PTFLYKVVR] fused to the C-terminus of translated protein.
Proper citation: RRID:Addgene_37954 Copy
Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22722250
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38001 Copy
Species: Homo sapiens
Genetic Insert: YTHDF2 YTH domain family, member 2
Vector Backbone Description: Backbone Size:5496; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681889
Proper citation: RRID:Addgene_38089 Copy
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