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Species: Homo sapiens
Genetic Insert: Rab4BS22N
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_49887 Copy
Species: Homo sapiens
Genetic Insert: Rab4B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_49886 Copy
Species: Homo sapiens
Genetic Insert: Rab6B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_49889 Copy
Species: Homo sapiens
Genetic Insert: Arf6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:EGFP N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_49883 Copy
Species: Homo sapiens
Genetic Insert: Connexin 43
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pegfp-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24210816
Proper citation: RRID:Addgene_49860 Copy
Species: Synthetic
Genetic Insert: plamodium falciparum TRAP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23236185
Proper citation: RRID:Addgene_50048 Copy
Species: Synthetic
Genetic Insert: pfTRAP(vwa)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23236185
Proper citation: RRID:Addgene_50049 Copy
Species: toxoplasma gondii
Genetic Insert: M2AP
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The first 46aa consist of a pro-peptide that gets cleaved during maturation.
Proper citation: RRID:Addgene_50051 Copy
Species: toxoplasma gondii
Genetic Insert: MIC2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pIRES2-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please note that Addgene has not been unable to verify the N357S, N463S, N470D mutations in the MIC2 sequence.
Proper citation: RRID:Addgene_50050 Copy
Species: Mycobacterium marinum + Photinus pyralis
Genetic Insert: G13 promoter + Firefly luciferase Red Thermostable
Vector Backbone Description: Backbone Size:3995; Vector Backbone:pMV306; Vector Types:Bacterial Expression, Mycobacteria integrating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23633686
Comments: Note that there are some discrepancies between Addgene's quality control sequence and the depositor's assembled full sequence. The Robertson lab reports that these differences will not affect the function of the plasmid.
Proper citation: RRID:Addgene_49998 Copy
Species: Mycobacterium marinum + Photinus pyralis
Genetic Insert: G13 promoter + Firefly luciferase Red Thermostable
Vector Backbone Description: Backbone Size:3995; Vector Backbone:pMV306; Vector Types:Bacterial Expression, Mycobacteria integrating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23633686
Comments: Note that there are some discrepancies between Addgene's quality control sequence and the depositor's assembled full sequence. The Robertson lab reports that these differences will not affect the function of the plasmid.
Proper citation: RRID:Addgene_49997 Copy
Species: Mus musculus
Genetic Insert: Crk-II
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2549; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25478005
Comments: Crk-II isolated from adult mouse kidney cDNA
Proper citation: RRID:Addgene_50024 Copy
Species: Reptilian orthoreovirus
Genetic Insert: p14FAST
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:2549; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25478005
Comments: When p14FAST is expressed in mammalian cells, fusion takes place between expressing cells and any other neighbouring cells that are in contact (even with non-expressing cells of a different cell type). Potential users should note that this implies a risk that cells carrying the fusion gene might fuse with cells of living humans, and potentially carry dangerous genetic material such as oncogenes and viruses into them (even basic cells lines carry mutations making them proliferative and immortal). Appropriate precautions should therefore be taken when handling the cultures.
Proper citation: RRID:Addgene_50025 Copy
Species: Homo sapiens
Genetic Insert: TRBP
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23658827
Comments: Fragment encoding a full length human TRBP was amplified using a cDNA mixture of total RNA extracted from human HeLa cells by PCR with primers containing restriction enzyme sites of HindIII and NotI. The amplified fragment was digested with the same enzyme sites and cloned into pcDNA3.1-myc/His A vector (Invitrogen). C-terminal myc-tagged TRBP fragment was amplified from pcDNA3.1-TRBP using primer with restriction enzyme sites NheI and HindIII. The amplified fragments were digested with same enzyme sites and cloned into pET-28a vector (Novagen).
Proper citation: RRID:Addgene_50351 Copy
Species: E coli
Genetic Insert: PanK
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15795230
Proper citation: RRID:Addgene_50386 Copy
Species: E coli
Genetic Insert: DPCK
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12372838
Proper citation: RRID:Addgene_50390 Copy
Species: Homo sapiens
Genetic Insert: Paxillin
Vector Backbone Description: Backbone Marker:Yamada lab ; Vector Backbone:pCFP NBC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Vector is a Clontech C1 vector with an MCS modification with original Bam HI mutation
Proper citation: RRID:Addgene_50510 Copy
Species: Gallus gallus
Genetic Insert: Vinculin
Vector Backbone Description: Backbone Marker:Yamada Lab; Vector Backbone:pmCherry NBC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Replacement of Clontech pEGFP C1 vector EGFP with mCherry and MCS modification with Bam HI mutation of original Bam H I.
Proper citation: RRID:Addgene_50527 Copy
Species: Homo sapiens
Genetic Insert: PXN paxillin
Vector Backbone Description: Backbone Marker:Yamada Lab; Vector Backbone:pmCherry NBC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Replacement of Clontech pEGFP C1 vector EGFP with mCherry and MCS modification with Bam HI mutation of original Bam H I.
Proper citation: RRID:Addgene_50526 Copy
Genetic Insert: gene encoding for MagFRET-1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24312622
Proper citation: RRID:Addgene_50742 Copy
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