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Species: Synthetic
Genetic Insert: UP Flank Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41331; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119613 Copy
Species: Synthetic
Genetic Insert: AbR Down Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH1276; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119616 Copy
Species: Synthetic
Genetic Insert: AbR Up Linker
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41331 ; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119617 Copy
Species: Synthetic
Genetic Insert: Pcat
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Promoter for the chloramphenicol acetyl transferase (CAT) gene in Salmonella enterica. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119600 Copy
Species: Synthetic
Genetic Insert: J23108_TSS
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119605 Copy
Species: Synthetic
Genetic Insert: Ptic
Vector Backbone Description: Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30819783
Comments: Synthetic variant of the tic promoter from E. coli. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119603 Copy
Species: Synthetic
Genetic Insert: J23103_TSS
Vector Backbone Description: Backbone Size:2247; Vector Backbone:PICH41295; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30819783
Comments: Addgene QC NGS identified sequence discrepancies in the plasmid insert. The plasmid is expected to function as described in the associated publication.
Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119604 Copy
Species: Synthetic
Genetic Insert: mKelly1
Vector Backbone Description: Backbone Size:5153; Vector Backbone:pET-53-DEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30425172
Proper citation: RRID:Addgene_119782 Copy
Species: Synthetic
Genetic Insert: CaMPARI2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4267; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30361563
Proper citation: RRID:Addgene_119723 Copy
Species: Synthetic
Genetic Insert: sgGFP
Vector Backbone Description: Backbone Marker:Broad Institute; Vector Backbone:pXRP_BRD003; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30059005
Proper citation: RRID:Addgene_119876 Copy
Species: Synthetic
Genetic Insert: CymRV5-nls-GFP
Vector Backbone Description: Backbone Size:5152; Vector Backbone:ePiggyBac-UbC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Plasmid contains ICP22 NLS with a single aa change that does not affect function. Sequence: RRPAVRSPPLGTRKRK.
Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119906 Copy
Species: Synthetic
Genetic Insert: cuOx144 array
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pDEST-Bxb1attB-loxP-PGK-puro-loxP; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Please note there are a few ambiguous bases in Addgene's NGS result. This is due to NGS having difficulty assembling repetitive sequences.
The repetitive sequences in this plasmid can be unstable while propagated in bacteria. It is recommended that the repetitive insert be verified by restriction digest and repeat counts be viewed as an approximation.
Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119903 Copy
Species: Synthetic
Genetic Insert: HA-tdMS2cp-tagRFP-Tx2-nls
Vector Backbone Description: Backbone Size:3526; Vector Backbone:ePiggyBac-UbC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119908 Copy
Species: Synthetic
Genetic Insert: TetRFlag-nls-tdTom
Vector Backbone Description: Backbone Size:5670; Vector Backbone:ePiggyBac-CAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31124784
Comments: Plasmid contains ICP22 NLS with a single aa change that does not affect function. Sequence: RRPAVRSPPLGTRKRK.
Please visit https://www.biorxiv.org/content/10.1101/409672v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_119909 Copy
Species: Synthetic
Genetic Insert: iGlul
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5933; Vector Backbone:pET41a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29735711
Proper citation: RRID:Addgene_119832 Copy
Species: Synthetic
Genetic Insert: iGluf
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5933; Vector Backbone:pET41a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29735711
Proper citation: RRID:Addgene_119833 Copy
Species: Synthetic
Genetic Insert: iGluh
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5933; Vector Backbone:pET41a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29735711
Proper citation: RRID:Addgene_119830 Copy
Species: Synthetic
Genetic Insert: C. elegans codon optimized GFPnovo2
Vector Backbone Description: Backbone Marker:Cori Bargmann's lab; Backbone Size:3600; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32550394
Comments: Please note that some discrepancies were found between Addgene's quality control and the depositor's full plasmid sequence. The depositor noted that the backbone was not sequenced, and these discrepancies do NOT affect plasmid function.
GFPnovo2 was originally generated by Arakawa et al., (2008)
Arakawa, H., Kudo, H., Batrak, V., Caldwell, R. B., Rieger, M. A., Ellwart, J. W., & Buerstedde, J.-M. (2008). Protein evolution by hypermutation and selection in the B cell line DT40. Nucleic Acids Research, 36(1), e1. http://doi.org/10.1093/nar/gkm616
Proper citation: RRID:Addgene_116943 Copy
Species: Synthetic
Genetic Insert: g3c
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30127387
Proper citation: RRID:Addgene_116893 Copy
Species: Synthetic
Genetic Insert: g3c
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30127387
Proper citation: RRID:Addgene_116891 Copy
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