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Species: Shewanella halifaxensis
Genetic Insert: ThsS
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:28373240
Proper citation: RRID:Addgene_90956 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pDEST-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43901 Copy
Species: Synthetic
Genetic Insert: QQR ZFN
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: QQR ZFN reference:
Even-Faitelson L, Samach A, Melamed-Bessudo C, Avivi-Ragolsky N, Levy AA (2011) Localized egg-cell expression of effector proteins for targeted modification of the Arabidopsis genome. The Plant Journal 68 (5):929-937. doi:10.1111/j.1365-313X.2011.04741.x
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
Proper citation: RRID:Addgene_44463 Copy
Species: Synthetic
Genetic Insert: AtCRU3 T494 TALENs
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: pZHY013 reference:
Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF (2012) TALENs enable efficient plant genome engineering. Plant Physiology. doi:10.1104/pp.112.205179
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
Proper citation: RRID:Addgene_44464 Copy
Species: Synthetic
Genetic Insert: Citrine-PTS1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10404; Vector Backbone:pMpGWB102; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23421791
Proper citation: RRID:Addgene_44592 Copy
Vector Backbone Description: Vector Backbone:pYPQ141-ZmUbi-RZ-Lb; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36127876
Proper citation: RRID:Addgene_173926 Copy
Genetic Insert: NLS-Cas12j2-NLS
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36127876
Proper citation: RRID:Addgene_173924 Copy
Genetic Insert: UNG-rAPOBEC1(R33A)-zCas9(D10A)
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_173997 Copy
Genetic Insert: rAPOBEC1-zCas9(D10A)-UNG
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_173998 Copy
Genetic Insert: rAPOBEC1-zCas9(D10A)-rXRCC1
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_173999 Copy
Genetic Insert: UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
Vector Backbone Description: Vector Backbone:pNJB91; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34713268
Proper citation: RRID:Addgene_174000 Copy
Genetic Insert: Cas12j2
Vector Backbone Description: Vector Backbone:pYPQ167; Vector Types:Plant Expression, CRISPR, Gateway compatible Cas12j2 entry clone; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36127876
Proper citation: RRID:Addgene_189780 Copy
Genetic Insert: gRNA cloning site
Vector Backbone Description: Vector Backbone:pYPQ141; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36127876
Proper citation: RRID:Addgene_189781 Copy
Species: PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli
Genetic Insert: PhoQ; lacI
Vector Backbone Description: Backbone Size:1885; Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35994658
Comments: Please note: Plasmid contains a S286L mutation in lacI. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_181952 Copy
Species: NarX - E. coli; lacI - E. coli
Genetic Insert: NarX; lacI
Vector Backbone Description: Backbone Size:1885; Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35994658
Proper citation: RRID:Addgene_181951 Copy
Species: Synthetic
Genetic Insert: er-mCherry
Vector Backbone Description: Backbone Size:11852; Vector Backbone:pMpGWB402; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35476214
Proper citation: RRID:Addgene_186288 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pSKunk-BLA; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35657952
Proper citation: RRID:Addgene_190243 Copy
Species: E. coli
Genetic Insert: Retron Eco1 Reverse Transcriptase
Vector Backbone Description: Vector Backbone:pJKR-O-mphR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35896746
Comments: A192P mutation in mphR codon optimized does not affect plasmid function.
Please visit https://doi.org/10.1101/2021.08.11.455990 for bioRxiv preprint.
Proper citation: RRID:Addgene_187202 Copy
Genetic Insert: chPheRS2 for DOPA
Vector Backbone Description: Backbone Size:4067; Vector Backbone:pUltra; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34942167
Proper citation: RRID:Addgene_182257 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: CcmO-PDT
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34232633
Proper citation: RRID:Addgene_165584 Copy
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