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Species: Homo sapiens
Genetic Insert: Human CYP3A4 proximal enhancer sgRNA for activation
Vector Backbone Description: Vector Backbone:lenti-sgRNA(MS2)_zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35452598
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.03.24.485641v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_176839 Copy
Species: Mus musculus
Genetic Insert: Sam68
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9341174
Proper citation: RRID:Addgene_17690 Copy
Species: Homo sapiens
Genetic Insert: ITGB2
Vector Backbone Description: Backbone Marker:Invitrogen (V36620); Backbone Size:5983; Vector Backbone:pcDNA6.2/EmGFP-Bsd/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995257
Comments: ITGB2 (BC157006) expressed under the control of CMV promoter and EmGFP-Bsd under the control of PGK
Proper citation: RRID:Addgene_176983 Copy
Species: Synthetic
Genetic Insert: PE2-NG variant
Vector Backbone Description: Vector Backbone:pLenti-PE2-BSD; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34446856
Proper citation: RRID:Addgene_176933 Copy
Species: SARS-CoV-2
Genetic Insert: S-protein B.1.617.2
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_177097 Copy
Species: Rattus norvegicus
Genetic Insert: T alpha 1p
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Proper citation: RRID:Addgene_17707 Copy
Species: Homo sapiens
Genetic Insert: rent1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12228722
Proper citation: RRID:Addgene_17708 Copy
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839358
Comments: pCLE includes a modified 5' LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).
Proper citation: RRID:Addgene_17703 Copy
Genetic Insert: CBFRE
Vector Backbone Description: Backbone Size:3600; Vector Backbone:na; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17721509
Comments: CBFRE: four CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the CBF1 binding element is GATCTGGAGTAACACGCCGTGGGAAAAAATTTATG. The binding site within this sequence is GTGGGAA.
A SmaI+XbaI double digest should yield 3.6kb and approx. 1.2kb bands. See Reviews link in right-hand column to view Addgene's diagnostic digest of this plasmid.
Proper citation: RRID:Addgene_17705 Copy
Species: Homo sapiens
Genetic Insert: hTERT
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9843956
Proper citation: RRID:Addgene_1772 Copy
Species: Homo sapiens
Genetic Insert: YTHDC1
Vector Backbone Description: Vector Backbone:modified from Addgene 61425; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34375583
Proper citation: RRID:Addgene_177129 Copy
Species: Homo sapiens
Genetic Insert: S6K2
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10490847
Comments: V390M is present, but should not affect protein function
Proper citation: RRID:Addgene_17730 Copy
Species: Danio rerio
Genetic Insert: Beta-actin
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Antisense probe cut with EcoR1 and transcribe with T7.
Sense probe cut with Xho1 and transcribe with T3.
Plasmid History:
Constructed by Greg Kelly 5/91.
Proper citation: RRID:Addgene_16932 Copy
Species: Arabidopsis thaliana
Genetic Insert: auxin-inducible degron (aid)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169356 Copy
Genetic Insert: pLeft
Vector Backbone Description: Vector Backbone:pMV306; Vector Types:Unspecified; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_169410 Copy
Species: Culex quinquefasciatus
Genetic Insert: pVMG0149_Cq-U6-6_2xBbsI-gRNA
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:3929; Vector Backbone:pCR2.1; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34017003
Proper citation: RRID:Addgene_169323 Copy
Species: Homo sapiens
Genetic Insert: Fucci(CA)
Vector Backbone Description: Vector Backbone:pCDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34916672
Proper citation: RRID:Addgene_169338 Copy
Vector Backbone Description: Backbone Size:6472; Vector Backbone:pPS04; Vector Types:Allelic exchange vector; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_169472 Copy
Genetic Insert: dCas9-HaloTag
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30318144
Proper citation: RRID:Addgene_169448 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4676; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34226556
Comments: Note that although the plasmid encodes a hygromycin resistance gene, it is not expressed from the plasmid. It is only expressed after Flp recombinase-mediated chromosomal integration of the plasmid.
Proper citation: RRID:Addgene_169744 Copy
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