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Species: Homo sapiens
Genetic Insert: ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing (PE3); Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 and MTOR to perform coselection for MTOR hyperactivating mutation E2419K. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173211 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G4 Q118R pegRNA
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: Prime editing vector derived from pU6-pegRNA-GG-acceptor. See Anzalone et al. Nature (2019) for the detailed pegRNA cloning protocol to target your gene of interest. False-positive colonies will appear pink or red if the RFP insert has not been replaced by the new pegRNA cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173199 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1-T804N mScarlet-I donor
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: This plasmid can be used in combination with pX330_ATP1A1_G7 or eSpCas9(1.1)_No_FLAG_ATP1A1_G7 to target mScarlet-I to ATP1A1 intron 17. A user-specified transgene of interest can also be cloned using restriction cloning sites upstream (KpnI, AflII, NcoI) and downstream (XhoI, PmeI, SbfI) of the mSc-TOSI gene cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173207 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: ATP1A1 G7 sgRNA target ATP1A1 intron 17 and this plasmid can be used to perform coselection for HDR in human cells. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 and user-specified sgRNA can be cloned using the BbsI sites. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173206 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: ATP1A1 G3 sgRNA target ATP1A1 intron 4 and this plasmid can be used to perform coselection for HDR in human cells. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 and user-specified sgRNA can be cloned using the BbsI sites. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173205 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G7 sgRNA + SpCas9
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: ATP1A1 G7 sgRNA target ATP1A1 intron 17 and this plasmid can be used to directly target a transgene to the ATP1A1 locus itselft, including the mTOR Signaling Indicator (TOSI) using ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor.
This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173204 Copy
Species: Homo sapiens
Genetic Insert: Glycerol-3-Phosphate Acyltransferase 4
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30846317
Proper citation: RRID:Addgene_173168 Copy
Species: Homo sapiens
Genetic Insert: ATP1A1 G2 sgRNA + user-specified sgRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36207338
Comments: U6-sgRNA cassette derived from pX330-U6-Chimeric_BB-CBh-hSpCas9. New user-specified sgRNA can be cloned using the BbsI sites. This vector can be used for adenine base editing or NHEJ-based editing to co-target ATP1A1 and a gene of interest. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
Proper citation: RRID:Addgene_173201 Copy
Species: Homo sapiens
Genetic Insert: Calcineurin Like EF-Hand Protein 1
Vector Backbone Description: Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30846317
Proper citation: RRID:Addgene_173166 Copy
Species: Homo sapiens
Genetic Insert: KCNQ3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5130; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35104249
Proper citation: RRID:Addgene_173163 Copy
Species: Homo sapiens
Genetic Insert: LgBiT-hACE2-IgG1 Fc
Vector Backbone Description: Backbone Size:4200; Vector Backbone:phCMV3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34183368
Proper citation: RRID:Addgene_173431 Copy
Species: Homo sapiens
Genetic Insert: KCNQ2
Vector Backbone Description: Backbone Size:5671; Vector Backbone:pCS2_IRES2_CyOFP1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33544076
Proper citation: RRID:Addgene_173155 Copy
Species: Homo sapiens
Genetic Insert: PGRMC2
Vector Backbone Description: Vector Backbone:pCMV-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34645803
Proper citation: RRID:Addgene_173468 Copy
Species: Homo sapiens
Genetic Insert: PGRMC1
Vector Backbone Description: Vector Backbone:pCMV-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34645803
Proper citation: RRID:Addgene_173467 Copy
Species: Homo sapiens
Genetic Insert: 4EHP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Proper citation: RRID:Addgene_17347 Copy
Species: Homo sapiens
Genetic Insert: 4EHP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Proper citation: RRID:Addgene_17348 Copy
Species: Homo sapiens
Genetic Insert: 4EHP
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17289916
Proper citation: RRID:Addgene_17349 Copy
Species: Homo sapiens
Genetic Insert: PGRMC1
Vector Backbone Description: Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34645803
Proper citation: RRID:Addgene_173473 Copy
Species: Homo sapiens
Genetic Insert: PGRMC1
Vector Backbone Description: Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34645803
Proper citation: RRID:Addgene_173472 Copy
Species: Homo sapiens
Genetic Insert: PGRMC1/PGRMC2 fusion
Vector Backbone Description: Vector Backbone:pCMV-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34645803
Proper citation: RRID:Addgene_173471 Copy
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