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Species: Saccharomyces cerevisiae
Genetic Insert: Kex2-FRBx2-msGFPx3
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247739 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CFP-FKBP-Inp54p
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16990515
Proper citation: RRID:Addgene_20155 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UPR-RFP
Vector Backbone Description: Backbone Size:4835; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19026441
Comments: There is a 2 base gap between depositor's sequence and Addgene sequence; per depositor this gap is in the promoter region and shouldn't significantly affect the plasmid.
Proper citation: RRID:Addgene_20132 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eroGFP
Vector Backbone Description: Backbone Size:4835; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19026441
Proper citation: RRID:Addgene_20131 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUMO-conjugating enzyme Ubc9
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_20369 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLPe recombinase
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pBS; Vector Types:Mammalian Expression, FLP/FRT; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16400644
Comments: Please note that this plasmid runs as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. If you need the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.
Proper citation: RRID:Addgene_20733 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_21052 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnq1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6600; Vector Backbone:pESC-URA; Vector Types:Yeast Expression, yeast/bacteria shuttle; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18756251
Proper citation: RRID:Addgene_21051 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4 DBD
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4628; Vector Backbone:pIZT; Vector Types:Insect Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Tetracycline
Defining Citation: PMID:19481053
Proper citation: RRID:Addgene_21602 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VPS2
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21494 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps20 Delta_C
Vector Backbone Description: Backbone Size:5549; Vector Backbone:pHis2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19234443
Proper citation: RRID:Addgene_21491 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Kss1-as2-trunc
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079053
Proper citation: RRID:Addgene_21790 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CFP-Ste12
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pACL7-YFP-A206K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079053
Comments: Construction was complicated; please refer to parent article's supplemental materials for details of construction. KpnI site is not unique in this plasmid.
Proper citation: RRID:Addgene_21787 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CPY*
Vector Backbone Description: Vector Backbone:p415 GAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39661468
Comments: Please visit https://doi.org/10.1101/2024.11.14.623614 for bioRxiv preprint.
Proper citation: RRID:Addgene_209539 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: msp1
Vector Backbone Description: Vector Backbone:pET-DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32541053
Proper citation: RRID:Addgene_226892 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Lifeact-3xmNeonGreen::URA3
Vector Backbone Description: Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39024291
Proper citation: RRID:Addgene_227595 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rpn11
Vector Backbone Description: Vector Backbone:pET DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28844860
Proper citation: RRID:Addgene_227622 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rpn11
Vector Backbone Description: Vector Backbone:pET-DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24463465
Proper citation: RRID:Addgene_227653 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rpn11
Vector Backbone Description: Vector Backbone:pET-DUET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24463465
Proper citation: RRID:Addgene_227655 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: rpn5
Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31778111
Proper citation: RRID:Addgene_227660 Copy
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