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Species: Mus musculus
Genetic Insert: 1.5kb upstream krt5
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59281 Copy
Species: Mus musculus
Genetic Insert: Intergenic 2kb upstream krt18
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59280 Copy
Species: Mus musculus
Genetic Insert: Cbx1
Vector Backbone Description: Backbone Marker:van Steensel lab, Addgene plasmid 59204; Vector Backbone:pINDgw RFA-V5-EcoDam; Vector Types:Mammalian Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17038565
Proper citation: RRID:Addgene_59203 Copy
Species: Mus musculus
Genetic Insert: Krt7-Intron 2
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59286 Copy
Species: Mus musculus
Genetic Insert: mFlg int-Intron 1
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59288 Copy
Species: Chlamydomonas noctigama
Genetic Insert: ChrimsonR-tdTomato
Vector Backbone Description: Backbone Size:4368; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: Plasmid is completely sequenced by the depositing lab except for part of the 3' ITR.
Proper citation: RRID:Addgene_59171 Copy
Vector Backbone Description: Backbone Marker:DGRC; Backbone Size:8780; Vector Backbone:pCasper-hs; Vector Types:Insect Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10748524
Comments: myc epitope tag serves as a linker peptide between dam and its fusion partner. This tag can be used to detect the fusion proteins by Western blotting or immunofluorescence microscopy to confirm their correct size and localization. Note that in order to obtain a detectable amount of protein, the fusion protein should be overexpressed by heat shock induction.
This vector cannot be used to express the myc-Dam protein by itself, since there is no startcodon.
Proper citation: RRID:Addgene_59218 Copy
Vector Backbone Description: Backbone Marker:DGRC; Backbone Size:8780; Vector Backbone:pCasper-hs; Vector Types:Insect Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10748524
Comments: myc epitope tag serves as a linker peptide between dam and its fusion partner. This tag can be used to detect the fusion proteins by Western blotting or immunofluorescence microscopy to confirm their correct size and localization. Note that in order to obtain a detectable amount of protein, the fusion protein should be overexpressed by heat shock induction.
This vector can also be used to express the Dam-myc protein by itself (there is a stopcodon 15 aa after the myc-tag).
Proper citation: RRID:Addgene_59217 Copy
Species: Mus musculus
Genetic Insert: Lmo3.8 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21599714
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59294 Copy
Species: Synthetic
Genetic Insert: QuasAr2-Citrine eFRET
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9235; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25118186
Proper citation: RRID:Addgene_59172 Copy
Species: Mus musculus
Genetic Insert: Lmo4.2 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20618444
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59293 Copy
Species: Synthetic
Genetic Insert: QuasAr2-mRuby2 eFRET
Vector Backbone Description: Backbone Marker:Pavel Osten; Backbone Size:9235; Vector Backbone:FCK(1.3); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25118186
Proper citation: RRID:Addgene_59174 Copy
Species: Mus musculus
Genetic Insert: Lmo3.5 shRNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11795; Backbone Size:7650; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21599714
Comments: Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.
Proper citation: RRID:Addgene_59295 Copy
Species: Mus musculus
Genetic Insert: Cbx1
Vector Backbone Description: Backbone Marker:PMID 9733856 ; Vector Backbone:HIV-CS-CG; Vector Types:Mammalian Expression, Lentiviral, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17038565
Comments: This vector may be used as a positive control. It expresses the Cbx1-V5-Dam protein.
Proper citation: RRID:Addgene_59211 Copy
Species: Caenorhabditis elegans
Genetic Insert: Blasticidin S resistance
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:1961; Vector Backbone:pBluescript KS(+); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24879462
Proper citation: RRID:Addgene_59181 Copy
Species: Mus musculus
Genetic Insert: krt5 ex1
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59229 Copy
Species: Arabidopsis thaliana
Genetic Insert: Guide RNA Cassette
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26479970
Proper citation: RRID:Addgene_59188 Copy
Species: Drosophila melanogaster
Genetic Insert: HP1
Vector Backbone Description: Backbone Marker:van Steensal lab; Vector Backbone:pNDam-myc, Addgene plasmid 59217; Vector Types:Insect Expression, DamID; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10748524
Proper citation: RRID:Addgene_59221 Copy
Species: Mus musculus
Genetic Insert: Krt7-ex6
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59236 Copy
Species: Mus musculus
Genetic Insert: Krt7-ex2
Vector Backbone Description: Backbone Size:7967; Vector Backbone:Brachyury-eGFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the final plasmid sequence is post-Gateway recombination. The plasmid contains attB sites (not attR sites as the map implies).
Proper citation: RRID:Addgene_59235 Copy
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