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Species: Mus musculus
Genetic Insert: Alpha Catulin
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26769899
Proper citation: RRID:Addgene_118979 Copy
Species: Homo sapiens
Genetic Insert: death-domain associated protein (Daxx)
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23405218
Proper citation: RRID:Addgene_119021 Copy
Species: Piper methysticum
Genetic Insert: Pm4CL1
Vector Backbone Description: Backbone Size:5336; Vector Backbone:pHis8-4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31332312
Proper citation: RRID:Addgene_119022 Copy
Species: Rattus norvegicus
Genetic Insert: GABRA1
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16181787
Proper citation: RRID:Addgene_118952 Copy
Species: Rattus norvegicus
Genetic Insert: GABRA5
Vector Backbone Description: Vector Backbone:pEGFP N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_118956 Copy
Species: Arabidopsis thaliana
Genetic Insert: SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
Vector Backbone Description: Vector Backbone:pRI 201-AN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30444549
Comments: The Eevee Linker present in this plasmid was invented by Prof. Michiyuki Matsuda at Kyoto University. The Eevee Linker has been patented by Kyoto University. The plasmid backbone used to produce the SOMA sensor was a kind gift of Michiyuki Matsuda and Kazuhiro Aoki.
Proper citation: RRID:Addgene_118940 Copy
Species: Mus musculus
Genetic Insert: OptoTGFBRs
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3925; Vector Backbone:ptdToamto-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29241005
Proper citation: RRID:Addgene_118942 Copy
Species: Homo sapiens
Genetic Insert: iRFP682-Smad2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:piRFP682-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29241005
Proper citation: RRID:Addgene_118943 Copy
Species: Arabidopsis thaliana
Genetic Insert: SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
Vector Backbone Description: Vector Backbone:pRI 201-AN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30444549
Comments: The Eevee Linker present in this plasmid was invented by Prof. Michiyuki Matsuda at Kyoto University. The Eevee Linker has been patented by Kyoto University. The plasmid backbone used to produce the SOMA sensor was a kind gift of Michiyuki Matsuda and Kazuhiro Aoki. Please note there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_118939 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Naweed Lab; Backbone Size:10383; Vector Backbone:pFGL1010; Vector Types:Fungal expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31363034
Proper citation: RRID:Addgene_118996 Copy
Vector Backbone Description: Backbone Marker:Naweed Lab; Backbone Size:6845; Vector Backbone:pFGL815N; Vector Types:Agrobacterium tumerfaciens-mediated transformation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31363034
Proper citation: RRID:Addgene_118991 Copy
Vector Backbone Description: Backbone Size:4803; Vector Backbone:mRuby2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: 5' site: BsrGI (not destroyed), 3' site: BsrGI (destroyed)
Proper citation: RRID:Addgene_118980 Copy
Vector Backbone Description: Backbone Size:4803; Vector Backbone:mRuby2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: 5' site: BsrGI (not destroyed), 3' site: BsrGI (destroyed)
Proper citation: RRID:Addgene_118981 Copy
Genetic Insert: VSVG
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9288971
Comments: The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. The Kozak sequence at the beginning of the GFP encoding region of the pEGFP-N1 plasmid from Clontech has been disrupted. Reference for the A206K mutation is Zacharias, D. A., Violin, J. D., Newton, A. C., and Tsien, R. Y. (2002) Science 296, 913-6. Reference for PAGFP is Patterson, G. H., and Lippincott-Schwartz, J. (2002) Science 297, 1873-7.
Proper citation: RRID:Addgene_11915 Copy
Species: Aequorea victoria
Genetic Insert: PAGFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12228718
Comments: The pPAGFP-C1 was not published. It was prepared by removing the PAGFP cDNA from pPAGFP-N1 with an AgeI/BsrGI restriction digest and subcloning it into a similarly digested pEYFP-C1 (Clontech). The pPAGFP-C1 was confirmed by sequencing. If you use this construct in future publications, use your best judgement for citation.
The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent.
The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.
Proper citation: RRID:Addgene_11910 Copy
Species: Homo sapiens
Genetic Insert: PIS
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23263280
Proper citation: RRID:Addgene_119078 Copy
Species: Aequorea victoria
Genetic Insert: pPAGFP-N1
Vector Backbone Description: Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12228718
Comments: The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent.
The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.
Proper citation: RRID:Addgene_11909 Copy
Species: Homo sapiens
Genetic Insert: NAD(P)H dehydrogenase, quinone 1 (NQO1)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15809436
Proper citation: RRID:Addgene_119058 Copy
Species: Homo sapiens
Genetic Insert: GalT
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8670420
Comments: The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here.
Proper citation: RRID:Addgene_11929 Copy
Genetic Insert: CD3delta-YFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3961; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16103128
Comments: CD3 delta was PCR amplified from a plasmid, introducing flanking BglII and EcoRI sites, and cloned in Ub-R-YFP digested with BamHI and EcoRI.
Proper citation: RRID:Addgene_11951 Copy
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