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Genetic Insert: SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33743213
Proper citation: RRID:Addgene_169463 Copy
Genetic Insert: SARS-CoV Spike truncated to remove 18 amino acids
Vector Backbone Description: Vector Backbone:pTwist; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33743213
Proper citation: RRID:Addgene_169465 Copy
Species: Xenopus laevis
Genetic Insert: Engrailed-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Xenopus Probe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1679005
Comments: In situ probe cut with Xba1, transcribe with T3.
Proper citation: RRID:Addgene_16950 Copy
Vector Backbone Description: Backbone Marker:Addgene #89753; Vector Backbone:pU6-Cj-sgRNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751124
Proper citation: RRID:Addgene_169915 Copy
Species: Mus musculus
Genetic Insert: Grin1
Vector Backbone Description: Vector Backbone:gRNA backbone; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34161760
Proper citation: RRID:Addgene_169789 Copy
Species: Homo sapiens
Genetic Insert: UDP-N-acetylhexosamine pyrophosphorylase
Vector Backbone Description: Backbone Size:7500; Vector Backbone:pSin4 EF1a IRES Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34288588
Comments: This plasmid was generated in the lab a long time ago and we cannot locate detailed cloning information at this point.
Proper citation: RRID:Addgene_169891 Copy
Species: Homo sapiens
Genetic Insert: Vangl2/Strabismus
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: This is a wild type human VANGL2/Strabismuus, cloned into pCS2P+. Injected RNA in zebrafish embryos gives the expected convergent/extension phenotype (Chris Thorpe, unpub. observations).
Reference: Made by Yan Zhang by PCR for RTM. Cloned as a Cla1-Xho1 insert.
Proper citation: RRID:Addgene_16992 Copy
Species: Homo sapiens
Genetic Insert: Donor sequence to ACTB gene for HR
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2959; Vector Backbone:pBlueScript II SK (+); Vector Types:HR donor vector for human ACTB gene.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30733539
Proper citation: RRID:Addgene_169798 Copy
Species: Synthetic
Genetic Insert: OxLight1
Vector Backbone Description: Backbone Marker:Viral Vector Facility - University of Zurich; Backbone Size:4438; Vector Backbone:pAAV.hSynapsin1; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35145320
Proper citation: RRID:Addgene_169792 Copy
Genetic Insert: Scrambled guide RNA
Vector Backbone Description: Backbone Marker:Addgene Plasmid #52961; Backbone Size:12993; Vector Backbone:LentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30733539
Proper citation: RRID:Addgene_169795 Copy
Genetic Insert: spCas9 PE*
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33837189
Comments: Please visit https://doi.org/10.1101/2020.12.15.422970 for bioRxiv preprint.
Proper citation: RRID:Addgene_169850 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169940 Copy
Genetic Insert: guide RNAs targeting E1, E2, and E3 ubiquitin ligases, deubiquitinases, and control genes
Vector Backbone Description: Vector Backbone:lentiCRISPRv2; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32494016
Proper citation: RRID:Addgene_169942 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pInducer20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34019797
Proper citation: RRID:Addgene_169983 Copy
Species: Homo sapiens
Genetic Insert: CARD8
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34019797
Proper citation: RRID:Addgene_169975 Copy
Species: Synthetic
Genetic Insert: bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
Vector Backbone Description: Backbone Size:5238; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33927418
Comments: This is a bacterial codon-optimized SpCas9 (H840A) sequence within pET-28b backbone (Addgene #73018) fused to bipartite NLS, a 33-amino acid linker and the engineered M-MLV RT from the parental pCMV-PE2 plasmid (Addgene #132775). To this construct, a short GS linker and a 6xHis-tag are added to C terminus.
Proper citation: RRID:Addgene_170103 Copy
Species: Homo sapiens
Genetic Insert: ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
Vector Backbone Description: Backbone Marker:Custom; Backbone Size:6000; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35044296
Proper citation: RRID:Addgene_170136 Copy
Genetic Insert: ChroME2s-ST-P2A-NLS-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_170177 Copy
Genetic Insert: ChroME2s-P2A-H2B-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_170163 Copy
Species: Escherichia coli
Genetic Insert: Ec86 RT
Vector Backbone Description: Backbone Marker:Fraser lab; Vector Backbone:pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076284
Proper citation: RRID:Addgene_170185 Copy
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