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ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
 
Resource Report
Resource Website 		RRID:Addgene_173211 ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA Homo sapiens Ampicillin PMID:36207338 Control PE3 nick sgRNAs vector derived from ATP1A1_G3_Dual_sgRNA. This vector can be used as a positive control for prime editing (PE3) to co-target ATP1A1 and MTOR to perform coselection for MTOR hyperactivating mutation E2419K. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Prime editing (PE3); Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
ATP1A1_G4_Q118R_Dual_pegRNA
 
Resource Report
Resource Website 		RRID:Addgene_173199 ATP1A1 G4 Q118R pegRNA Homo sapiens Ampicillin PMID:36207338 Prime editing vector derived from pU6-pegRNA-GG-acceptor. See Anzalone et al. Nature (2019) for the detailed pegRNA cloning protocol to target your gene of interest. False-positive colonies will appear pink or red if the RFP insert has not been replaced by the new pegRNA cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
ATP1A1_T804N_hPGK1_mScarlet-I_Donor
 
Resource Report
Resource Website 		RRID:Addgene_173207 ATP1A1-T804N mScarlet-I donor Homo sapiens Ampicillin PMID:36207338 This plasmid can be used in combination with pX330_ATP1A1_G7 or eSpCas9(1.1)_No_FLAG_ATP1A1_G7 to target mScarlet-I to ATP1A1 intron 17. A user-specified transgene of interest can also be cloned using restriction cloning sites upstream (KpnI, AflII, NcoI) and downstream (XhoI, PmeI, SbfI) of the mSc-TOSI gene cassette. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
pX330_ATP1A1_G7_Dual_sgRNA
 
Resource Report
Resource Website 		RRID:Addgene_173206 ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9 Homo sapiens Ampicillin PMID:36207338 ATP1A1 G7 sgRNA target ATP1A1 intron 17 and this plasmid can be used to perform coselection for HDR in human cells. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 and user-specified sgRNA can be cloned using the BbsI sites. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
pX330_ATP1A1_G3_Dual_sgRNA
 
Resource Report
Resource Website 		RRID:Addgene_173205 ATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9 Homo sapiens Ampicillin PMID:36207338 ATP1A1 G3 sgRNA target ATP1A1 intron 4 and this plasmid can be used to perform coselection for HDR in human cells. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 and user-specified sgRNA can be cloned using the BbsI sites. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
pX330_ATP1A1_G7
 
Resource Report
Resource Website 		RRID:Addgene_173204 ATP1A1 G7 sgRNA + SpCas9 Homo sapiens Ampicillin PMID:36207338 ATP1A1 G7 sgRNA target ATP1A1 intron 17 and this plasmid can be used to directly target a transgene to the ATP1A1 locus itselft, including the mTOR Signaling Indicator (TOSI) using ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
pMXS-IRES-Blast HA-GPAT4
 
Resource Report
Resource Website 		RRID:Addgene_173168 Glycerol-3-Phosphate Acyltransferase 4 Homo sapiens Ampicillin PMID:30846317 Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin Synonymous mutations at sgRNA sites 2026-08-15 01:10:31 0
ATP1A1_G2_Dual_sgRNA
 
Resource Report
Resource Website 		RRID:Addgene_173201 ATP1A1 G2 sgRNA + user-specified sgRNA Homo sapiens Ampicillin PMID:36207338 U6-sgRNA cassette derived from pX330-U6-Chimeric_BB-CBh-hSpCas9. New user-specified sgRNA can be cloned using the BbsI sites. This vector can be used for adenine base editing or NHEJ-based editing to co-target ATP1A1 and a gene of interest. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint. Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
pMXS-IRES-Blast 3XFLAG-CHP1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_173166 Calcineurin Like EF-Hand Protein 1 Homo sapiens Ampicillin PMID:30846317 Backbone Marker:Cell Biolabs; Backbone Size:5600; Vector Backbone:pMXS-IRES-Blast; Vector Types:Retroviral; Bacterial Resistance:Ampicillin Synonymous mutations at sgRNA sites 2026-08-15 01:10:31 1
pcDNA5FRT_KCNQ3
 
Resource Report
Resource Website 		RRID:Addgene_173163 KCNQ3 Homo sapiens Ampicillin PMID:35104249 Backbone Marker:Invitrogen; Backbone Size:5130; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
LgBiT-hACE2
 
Resource Report
Resource Website 		RRID:Addgene_173431 LgBiT-hACE2-IgG1 Fc Homo sapiens Kanamycin PMID:34183368 Backbone Size:4200; Vector Backbone:phCMV3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin 2026-08-15 01:10:32 0
pCS2_IRES2_CyOFP1_KCNQ2 var4
 
Resource Report
Resource Website 		RRID:Addgene_173155 KCNQ2 Homo sapiens Ampicillin PMID:33544076 Backbone Size:5671; Vector Backbone:pCS2_IRES2_CyOFP1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
pCMV-FLAG-PGRMC2
 
Resource Report
Resource Website 		RRID:Addgene_173468 PGRMC2 Homo sapiens Ampicillin PMID:34645803 Vector Backbone:pCMV-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:32 0
pCMV-FLAG-PGRMC1-1-170
 
Resource Report
Resource Website 		RRID:Addgene_173467 PGRMC1 Homo sapiens Ampicillin PMID:34645803 Vector Backbone:pCMV-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Deleted for amino acids 171-194 2026-08-15 01:10:32 0
pFLAG-4EHP(K130R)
 
Resource Report
Resource Website 		RRID:Addgene_17347 4EHP Homo sapiens Ampicillin PMID:17289916 Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K130R 2026-08-15 01:10:32 0
pFLAG-4EHP(K134R)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_17348 4EHP Homo sapiens Ampicillin PMID:17289916 Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K134R 2026-08-15 01:10:32 1
pFLAG-4EHP(K121/130R)
 
Resource Report
Resource Website 		RRID:Addgene_17349 4EHP Homo sapiens Ampicillin PMID:17289916 Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFlag-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin K121/130R 2026-08-15 01:10:32 0
pCMV-HA-PGRMC1-21-195
 
Resource Report
Resource Website 		RRID:Addgene_173473 PGRMC1 Homo sapiens Ampicillin PMID:34645803 Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Deleted for amino acids 1-20 2026-08-15 01:10:32 0
pCMV-HA-PGRMC1-1-54
 
Resource Report
Resource Website 		RRID:Addgene_173472 PGRMC1 Homo sapiens Ampicillin PMID:34645803 Vector Backbone:pCMV-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Deleted for amino acids 55-194 2026-08-15 01:10:32 0
pCMV-FLAG-PGRMC-Hybrid3
 
Resource Report
Resource Website 		RRID:Addgene_173471 PGRMC1/PGRMC2 fusion Homo sapiens Ampicillin PMID:34645803 Vector Backbone:pCMV-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:32 0

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