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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pcDNA3.2/V5 cand142 Resource Report Resource Website |
RRID:Addgene_26322 | cand142 | Homo sapiens | Ampicillin | PMID:20413612 | pre-miRNA hairpins and the surrounding regions were amplified from human genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST. | Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:11 | 0 | |
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pcDNA3.2/V5 mmu-mir-1197 Resource Report Resource Website |
RRID:Addgene_26339 | mmu-mir-1197 | Mus musculus | Ampicillin | PMID:20413612 | pre-miRNA hairpins and the surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST. | Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:11 | 0 | |
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pcDNA3.2/V5 mmu-mir-224 Resource Report Resource Website |
RRID:Addgene_26335 | mmu-mir-224 | Mus musculus | Ampicillin | PMID:20413612 | pre-miRNA hairpins and the surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST. | Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:11 | 0 | |
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pcDNA3.2/V5 mmu-mir-375 Resource Report Resource Website |
RRID:Addgene_26336 | mmu-mir-375 | Mus musculus | Ampicillin | PMID:20413612 | pre-miRNA hairpins and the surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST. | Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:11 | 0 | |
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pcDNA3.2/V5 mmu-mir-153 Resource Report Resource Website |
RRID:Addgene_26330 | mmu-mir-153 | Mus musculus | Ampicillin | PMID:20413612 | pre-miRNA hairpins and the surrounding regions were amplified from mouse BL6 genomic DNA using Pfu Polymerase and primers with Gateway (Invitrogen)- compatible ends designed to anneal about 100 nt upstream of and downstream from the miRNA hairpins. PCR products were inserted into Gateway vector pDONR221 and subsequently into pcDNA3.2/V5-DEST. | Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:11 | 0 | |
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MDC1 in pENTR4 (330-5) Resource Report Resource Website 1+ mentions |
RRID:Addgene_26427 | Mediator of DNA damage checkpoint 1 | Homo sapiens | Kanamycin | Backbone Marker:Invitrogen; Backbone Size:2477; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:12 | 2 | |||
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pLenti CMV rtTA3 Blast (w756-1) Resource Report Resource Website 50+ mentions |
RRID:Addgene_26429 | Tetracycline repressor A3 mutant | Ampicillin | Use to make them Tet-On Advanced. | Backbone Size:7681; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 63 | |||
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pTitin Resource Report Resource Website 1+ mentions |
RRID:Addgene_26428 | partial sequence of mouse Titin | Mus musculus | Ampicillin | Backbone Size:7400; Vector Backbone:pRRL.SIN.cPPT.PGK.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 1 | |||
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pAMHDAdGT8-4 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26421 | HSV-TK, mCMV-b-gal | E.coli, HSV | Ampicillin | There are some discrepancies between Addgene's quality control sequence and the author's sequence, but the Mitani lab has confirmed that this plasmid is correct. The depositor has also confirmed that there is an extra SacI site present in the plasmid is not apparent in the theoretical sequence on which the map below is based. Addgene does not recommend using SacI for a diagnostic digest. | Backbone Size:3360; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 1 | ||
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pRG5271Neo Resource Report Resource Website |
RRID:Addgene_26387 | Caenorhabditis elegans | Ampicillin | PMID:20729841 | Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 0 | |||
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pDEST_FUS_G2A_R521H Resource Report Resource Website |
RRID:Addgene_26381 | FUS | Homo sapiens | Ampicillin | PMID:22081015 | Backbone Marker:N/A; Backbone Size:5299; Vector Backbone:pFRT_DESTFLAGHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | R521H | Addgene | 2026-10-31 02:08:12 | 0 | |
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bActin-RSG-BiSKi Resource Report Resource Website 1+ mentions |
RRID:Addgene_26437 | bActin promoter | unknown | Ampicillin | Backbone Size:4726; Vector Backbone:unknown; Vector Types:4726; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 1 | |||
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pLenti CMVtight Blast DEST (w762-1) Resource Report Resource Website 1+ mentions |
RRID:Addgene_26434 | no insert | Chloramphenicol and Ampicillin | Need to be transduced into a Tet-On Advanced cell line for expression of the GOI. There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function. | Backbone Size:9116; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin | Addgene | 2026-10-31 02:08:12 | 6 | |||
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flk1 promoter Resource Report Resource Website 1+ mentions |
RRID:Addgene_26436 | flk1 promoter | Danio rerio | Ampicillin | PMID:16251212 | From the paper: Approximately 6.5 kb of upstream sequence of the zebrafish flk1 gene (Liao et al., 1997; Thompson et al., 1998) was amplified from wild-type genomic DNA based on the sequence information previously deposited (from –6410 to –3 of the transcriptional start site, GenBank Accession Number AY045466) (Chan et al., 2002), and subcloned into the pCRII TOPO vector (Invitrogen). This fragment has been shown to drive endothelial-specific expression of the green-red coral fluorescent protein (G-RCFP) (Cross et al., 2003). The EcoRI cleaved fragment from this construct was placed 5′ upstream of the enhanced green fluorescent protein (EGFP) vector (Clontech) to drive endothelial specific expression of EGFP | Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Topo cloning vector; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 1 | |
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p164 Dync1i1.B Resource Report Resource Website |
RRID:Addgene_26435 | cytoplasmic dynein 1 intermediate chain 1 isoform B | Mus musculus | Kanamycin | PMID:20657784 | Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:subcloning and sequencing; Bacterial Resistance:Kanamycin | N/A | Addgene | 2026-10-31 02:08:12 | 0 | |
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pLenti CMVtight Puro DEST (w768-1) Resource Report Resource Website 1+ mentions |
RRID:Addgene_26430 | no insert | Chloramphenicol and Ampicillin | Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI. | Backbone Size:9402; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin | Addgene | 2026-10-31 02:08:12 | 7 | |||
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C32V Resource Report Resource Website 1+ mentions |
RRID:Addgene_26396 | Cerulean-32-Venus | Kanamycin | PMID:17040988 | This construct contains Cerulean attached via a 32 amino acid linker to Venus. See supplemental methods section in associated paper for further details of plasmid construction. | Backbone Marker:Clonetech; Backbone Size:3937; Vector Backbone:EGFP N1; Vector Types:Mammalian Expression, FRET standard; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:12 | 7 | ||
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pF152 pcDNA3.1(+)SOD1A4V Resource Report Resource Website 1+ mentions |
RRID:Addgene_26398 | SOD1 | Homo sapiens | Ampicillin | PMID:20221404 | The complete cDNA insert was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 4 of the cDNA from GCC to GTC, resulting in the A4V mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5’ and 3’ end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+). | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | A4V | Addgene | 2026-10-31 02:08:12 | 3 |
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pF148 pSOD1G37RAcGFP1 Resource Report Resource Website 10+ mentions |
RRID:Addgene_26409 | SOD1 | Homo sapiens | Kanamycin | PMID:20221404 | The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 37 of the cDNA from GGA to AGA, resulting in the G37R mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5’ of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | G37R | Addgene | 2026-10-31 02:08:12 | 10 |
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pF147 pSOD1A4VAcGFP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26408 | SOD1 | Homo sapiens | Kanamycin | PMID:20221404 | The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 4 of the cDNA from GCC to GTC, resulting in the A4V mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5’ of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | A4V | Addgene | 2026-10-31 02:08:12 | 5 |
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