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Species: Other
Genetic Insert: sgRNA cassette
Vector Backbone Description: Backbone Marker:Arlene Sharpe's Lab; Backbone Size:7095; Vector Backbone:pXPR_071; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38411617
Proper citation: RRID:Addgene_184859 Copy
Species: Other
Genetic Insert: GFP(-6)-H3
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205032 Copy
Species: Other
Genetic Insert: GFP(+6)-L1
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205039 Copy
Species: Other
Genetic Insert: GFP(0)-H2
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205037 Copy
Species: Other
Genetic Insert: GFP(0)-H1
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205036 Copy
Species: Other
Genetic Insert: GFP(+6)-L3
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205041 Copy
Species: Other
Genetic Insert: GFP(+6)-L2
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205040 Copy
Species: Other
Genetic Insert: mCherry(-6)-L1
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205046 Copy
Species: Other
Genetic Insert: mCherry(-6)-L3
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205048 Copy
Species: Other
Genetic Insert: mCherry(-6)-L2
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205047 Copy
Species: Other
Genetic Insert: GFP(-6)-H2_mCherry(-6)-L1
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38308651
Proper citation: RRID:Addgene_205050 Copy
Species: Other
Genetic Insert: ER-miniTurbo-V5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214452 Copy
Species: Other
Genetic Insert: Firefly luciferase
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214071 Copy
Species: Other
Genetic Insert: TurboRFP
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214063 Copy
Species: Other
Genetic Insert: TurboRFP
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214065 Copy
Species: Other
Genetic Insert: TurboRFP
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214066 Copy
Species: Other
Genetic Insert: TurboRFP
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214067 Copy
Species: Other
Genetic Insert: Firefly luciferase
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214074 Copy
Species: Other
Genetic Insert: Firefly luciferase
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214077 Copy
Species: Other
Genetic Insert: Firefly luciferase
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214078 Copy
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