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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
YFP-CL1
 
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1+ mentions
RRID:Addgene_11950 YFP-CL1 Kanamycin PMID:16103128 The YFP-CL1 reporter was generated by inserting an oligodimer encoding the 16 amino acid CL1 degradation signal (ACKNWFSSLSHFVIHL) in enhanced GFP-C1 (BD Biosciences Clontech) using the EcoRI and BamH1 restriction sites, then the enhanced GFP open-reading frame was replaced with YFP using the Nhe1 and SspB1 restriction sites. Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:03:12 3
Venus-GalT
 
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RRID:Addgene_11931 GalT Homo sapiens Kanamycin PMID:8670420 Venus was developed by Miyawaki and colleagues. The reference is Nagai, T., Ibata, K., Park, E. S., Kubota, M., Mikoshiba, K., and Miyawaki, A. (2002) Nat Biotechnol 20, 87-90. The EYFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here. Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Amino acids 1-60 of the human galactosyltransferase. Venus has the A206K mutation to disrupt self-association. 2026-08-15 01:03:13 6
Cerulean-GalT
 
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RRID:Addgene_11930 GalT Homo sapiens Kanamycin PMID:8670420 Cerulean ws developed by Piston lab. Reference is Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004) Nat Biotechnol 22, 445-9. The EGFP (Clontech Laboratories, Inc., Palo, Alto, CA) contains a valine immediately after the start codon that is not found in the wild type sequence. However, to avoid confusion with previously published work on GFP mutants, the wild type residue numbers are used. This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here. Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Amino acids 1-60 of the human galactosyltransferase. Cerulean has mutations described by Rizzo, M. A., Springer, G. H., Granada, B., and Piston, D. W. (2004) Nat Biotechnol 22, 445-9. 2026-08-15 01:03:13 5
pC0.057
 
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RRID:Addgene_119565 ParsB Other Kanamycin PMID:30819783 AsO2- inducible promoter from the arsB gene. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin bp 117 G to A, RBS* added (22 bp) upstream of ATG. 2026-08-15 01:03:13 0
pC0.034
 
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RRID:Addgene_119562 J23104MH Synthetic Kanamycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:03:13 0
pC0.041
 
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RRID:Addgene_119563 J23111MH Synthetic Kanamycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:03:13 0
ECFP-GalT
 
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RRID:Addgene_11937 GalT Homo sapiens Kanamycin PMID:8670420 This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 or pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here. Addgene Note: Please see the Addgene sequencing result to view the fluorophore sequence. Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Amino acids 1-60 of the human galactosyltransferase. ECFP has K27R and N165H mutation. 2026-08-15 01:03:12 4
EYFP-GalT
 
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RRID:Addgene_11936 GalT Homo sapiens Kanamycin PMID:8670420 This construct contains amino acids 1-60 of the human galactosyltransferase. The original article describes cloning into pcDNA1 ro pCDLSRa. A subsequent paper Zaal et. al. Cell 99:589-601 (1999) describes subcloning into the parent vector used here. Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Amino acids 1-60 of the human galactosyltransferase. 2026-08-15 01:03:12 2
PAGFP-Ub
 
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RRID:Addgene_11933 Ubiquitin Homo sapiens Kanamycin PMID:16606690 PAGFP is a photoactivatable version of GFP. It is essentially the same as the standard EGFP-C1 from clontech, but contains the mutations L64F/T65S/V163A/T203H, and conatins an A206K mutation to disrupt dimerization. In total, the PAGFP-Ubiquitin insert is 983bp. Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:03:12 2
GFP-Ub KO.G76V
 
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RRID:Addgene_11932 Ubiquitin KO.G76V Homo sapiens Kanamycin PMID:16606690 Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Conjugation deficient mutant. All 7 lysines of ubiquitin mutated to arginines. Glycine 76 mutated to valine. 2026-08-15 01:03:12 4
mRFP-Ub
 
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10+ mentions
RRID:Addgene_11935 Ubiquitin Homo sapiens Kanamycin PMID:16702407 Backbone Marker:Clontech; Backbone Size:4500; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:03:13 13
GFP-Ub KO
 
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RRID:Addgene_11934 Ubiquitin KO Homo sapiens Kanamycin PMID:16702407 Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin All 7 lysines of ubiquitin mutated to arginines. 2026-08-15 01:03:12 5
Ub-G76V-GFP
 
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10+ mentions
RRID:Addgene_11941 Ub-G76V-GFP Kanamycin PMID:10802622 The ubiquitin open reading frame was amplified by PCR from the Ub-Pro-Gal plasmid with the sense primer 5'-GCG GAATTCACCATGCAGATCTTCGTGAAGACT-3' and the antisense primer 5'-GCGGGATCCTGTCGACCAAGCTTC CCCACCACACCTCTGAGACGGAGTAC-3'. The PCR product was cloned into the EcoRI and BamHI sites of the EGFP-N1 vector from Clontech. Backbone Marker:Clontech; Backbone Size:3970; Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Ubiquitin fused to N-terminus of GFP. Glycine 76 mutated to Valine. 2026-08-15 01:03:14 44
pCA0.002
 
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RRID:Addgene_119560 Kanamycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:03:13 0
pC0.049
 
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RRID:Addgene_119561 BBa_J23119MH Synthetic Kanamycin PMID:30819783 Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:03:14 0
pJJDuet30
 
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1+ mentions
RRID:Addgene_11962 Ssp DnaE-N Kanamycin PMID:15908942 Backbone Marker:Invitrogen; Backbone Size:3600; Vector Backbone:pRSFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:03:13 2
pET-NEMO
 
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RRID:Addgene_11966 NEMO Homo sapiens Kanamycin PMID:16547522 Backbone Size:5370; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:03:14 4
pC.061
 
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RRID:Addgene_119603 Ptic Synthetic Kanamycin PMID:30819783 Synthetic variant of the tic promoter from E. coli. Please visit https://www.biorxiv.org/content/10.1101/426700v1 for bioRxiv preprint. Backbone Size:3313; Vector Backbone:pSB4K5; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:03:13 0
pEG302 5aa SunTag VP64 g4+g17 (FWA)
 
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RRID:Addgene_119672 g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS Kanamycin PMID:30760722 Vector Backbone:pEG302; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin 2026-08-15 01:03:14 0
pCas9-J23113
 
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RRID:Addgene_119701 Cas9 Streptococcus pyogenes Kanamycin PMID:29982721 Vector Backbone:p15A origin; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin 2026-08-15 01:03:14 0

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