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pRDA_526
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_184859 sgRNA cassette Other Ampicillin PMID:38411617 Backbone Marker:Arlene Sharpe's Lab; Backbone Size:7095; Vector Backbone:pXPR_071; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:28:51 1
pET-6xHis-GFP(-6)-H3
 
Resource Report
Resource Website 		RRID:Addgene_205032 GFP(-6)-H3 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:28:55 0
pET-6xHis-GFP(+6)-L1
 
Resource Report
Resource Website 		RRID:Addgene_205039 GFP(+6)-L1 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E16R, D86K, D112K, D127R, I138R, D207R, N222K, L246R 2026-08-15 01:28:54 0
pET-6xHis-GFP(0)-H2
 
Resource Report
Resource Website 		RRID:Addgene_205037 GFP(0)-H2 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E16R, I138R, D207R, N222K, L246R 2026-08-15 01:28:54 0
pET-6xHis-GFP(0)-H1
 
Resource Report
Resource Website 		RRID:Addgene_205036 GFP(0)-H1 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E16R, I138R, D207R, N222K, L246R 2026-08-15 01:28:54 0
pET-6xHis-GFP(+6)-L3
 
Resource Report
Resource Website 		RRID:Addgene_205041 GFP(+6)-L3 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E16R, D86K, D112K, D127R, I138R, D207R, N222K, L246R 2026-08-15 01:28:55 0
pET-6xHis-GFP(+6)-L2
 
Resource Report
Resource Website 		RRID:Addgene_205040 GFP(+6)-L2 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin E16R, D86K, D112K, D127R, I138R, D207R, N222K, L246R 2026-08-15 01:28:54 0
pET-6xHis-mCherry(-6)-L1
 
Resource Report
Resource Website 		RRID:Addgene_205046 mCherry(-6)-L1 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:28:54 0
pET-6xHis-mCherry(-6)-L3
 
Resource Report
Resource Website 		RRID:Addgene_205048 mCherry(-6)-L3 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:28:54 0
pET-6xHis-mCherry(-6)-L2
 
Resource Report
Resource Website 		RRID:Addgene_205047 mCherry(-6)-L2 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:28:55 0
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1
 
Resource Report
Resource Website 		RRID:Addgene_205050 GFP(-6)-H2_mCherry(-6)-L1 Other Ampicillin PMID:38308651 Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:28:55 0
p663-UBC-ER-miniTurbo-V5_IDG-K
 
Resource Report
Resource Website 		RRID:Addgene_214452 ER-miniTurbo-V5 Other Ampicillin These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags. Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin 2026-08-15 01:29:03 0
LRT
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_214071 Firefly luciferase Other Ampicillin PMID:38375914 Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint. Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Linker sequence present between firefly luciferase and GFP 2026-08-15 01:29:04 2
ART
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_214063 TurboRFP Other Ampicillin PMID:38375914 Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint. Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Linker sequence present between RFP and GFP 2026-08-15 01:29:04 2
ARTX_ctrl
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_214065 TurboRFP Other Ampicillin PMID:38375914 Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint. Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Remnant of linker sequence present at the end of RFP 2026-08-15 01:29:04 1
ART_RTctrl
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_214066 TurboRFP Other Ampicillin PMID:38375914 Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint. Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Linker sequence present between RFP and GFP 2026-08-15 01:29:04 1
AiRT
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_214067 TurboRFP Other Ampicillin PMID:38375914 Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint. Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Linker sequence present between RFP and GFP 2026-08-15 01:29:04 1
LRT_RTctrl
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_214074 Firefly luciferase Other Ampicillin PMID:38375914 Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint. Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Linker sequence present between firefly luciferase and GFP 2026-08-15 01:29:04 1
LiRTX_ctrl
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_214077 Firefly luciferase Other Ampicillin PMID:38375914 Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint. Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Remnant of linker sequence present at the end of firefly luciferase 2026-08-15 01:29:03 1
LiRT_RTctrl
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_214078 Firefly luciferase Other Ampicillin PMID:38375914 Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint. Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Linker sequence present between firefly luciferase and GFP 2026-08-15 01:29:04 1

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