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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pF146 pSOD1WTAcGFP1 Resource Report Resource Website 10+ mentions |
RRID:Addgene_26407 | SOD1 | Homo sapiens | Kanamycin | PMID:20221404 | The complete cDNA was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5’ of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:12 | 12 | |
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pENTR4_TAF15 Resource Report Resource Website |
RRID:Addgene_26368 | TAF15 | Homo sapiens | Kanamycin | PMID:21344536 | Backbone Marker:Invitrogen; Backbone Size:2278; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:12 | 0 | ||
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pENTR4_EWSR1 Resource Report Resource Website |
RRID:Addgene_26367 | EWSR1 | Homo sapiens | Kanamycin | PMID:21344536 | Backbone Marker:Invitrogen; Backbone Size:2278; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:12 | 0 | ||
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pF154 pcDNA3.1(+)SOD1G85R Resource Report Resource Website 1+ mentions |
RRID:Addgene_26400 | SOD1 | Homo sapiens | Ampicillin | PMID:20221404 | The complete cDNA insert was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 85 of the cDNA from GGC to CGC, resulting in the G85R mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5’ and 3’ end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+). | Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | G85R | Addgene | 2026-10-31 02:08:12 | 3 |
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pF142 pAcGFP1 SOD1 A4V Resource Report Resource Website 1+ mentions |
RRID:Addgene_26403 | SOD1 | Homo sapiens | Kanamycin | PMID:20221404 | The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 4 of the cDNA from GCC to GTC, resulting in the A4V mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pACGFPC1. Thus GFP is 5’ of the SOD1 insert and upstream in the GFP-SOD1 fusion protein. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | A4V | Addgene | 2026-10-31 02:08:12 | 1 |
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pF141 pAcGFP1 SOD1WT Resource Report Resource Website 10+ mentions |
RRID:Addgene_26402 | SOD1 | Homo sapiens | Kanamycin | PMID:20221404 | The complete cDNA was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-SalI 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pACGFPC1. Thus GFP is 5’ of the SOD1 insert and upstream in the GFP-SOD1 fusion protein. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-10-31 02:08:12 | 11 | |
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pET23(a)_mod Resource Report Resource Website |
RRID:Addgene_26363 | FLAG/HA | Ampicillin | PMID:21344536 | Backbone Marker:Stratagene; Backbone Size:3729; Vector Backbone:pET23(a); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 0 | |||
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pFRT_DESTFLAGHA Resource Report Resource Website 1+ mentions |
RRID:Addgene_26360 | Chloramphenicol and Ampicillin | PMID:21344536 | Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | Addgene | 2026-10-31 02:08:12 | 3 | ||||
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pFRT_DESTFLAGHA_eGFP Resource Report Resource Website |
RRID:Addgene_26362 | EGFP | Ampicillin | PMID:21344536 | Backbone Size:0; Vector Backbone:pFRT_DESTFLAGHA; Vector Types:Gateway Cloning; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 0 | |||
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pdestMB14 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26415 | attR4-ccdB-attR2 | Chloramphenicol and Ampicillin | PMID:15489340 | A KpnI-ApaI fragment containing the GFP encoding sequence and the unc-54 3′ UTR from pPD95.79 was cloned into pDP#MM016b. An EcoRV fragment of the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into this new construct digested with Acc65I and filled in with Klenow. The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms. | Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | Addgene | 2026-10-31 02:08:12 | 1 | ||
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pDEST_TO_TAF15 Resource Report Resource Website |
RRID:Addgene_26378 | TAF15 | Homo sapiens | Ampicillin | PMID:22081015 | Backbone Marker:N/A; Backbone Size:5421; Vector Backbone:pFRT_TO_DDESTFLAGHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 0 | ||
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pF150 pSOD1G93AAcGFP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26411 | SOD1 | Homo sapiens | Kanamycin | PMID:20221404 | The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 93 of the cDNA from GGT to GCT, resulting in the G93A mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5’ of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | G94A | Addgene | 2026-10-31 02:08:12 | 8 |
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pdestDD03 Resource Report Resource Website |
RRID:Addgene_26414 | attR4-ccdB-attR2 cassette | Chloramphenicol and Ampicillin | PMID:15489340 | A HindIII-KpnI fragment containing the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into the unc-119 rescue vector pDP#MM016b. The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms. | Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | Addgene | 2026-10-31 02:08:12 | 0 | ||
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pDEST8_FLAGHA_FUS_HIS Resource Report Resource Website |
RRID:Addgene_26375 | FUS | Homo sapiens | Ampicillin | PMID:22081015 | Backbone Marker:Invitrogen; Backbone Size:4749; Vector Backbone:pDEST8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | delta 527 (STOP codon) | Addgene | 2026-10-31 02:08:12 | 0 | |
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pDEST_FUS Resource Report Resource Website 1+ mentions |
RRID:Addgene_26374 | FUS | Homo sapiens | Ampicillin | PMID:22081015 | Backbone Marker:N/A; Backbone Size:5299; Vector Backbone:pFRT_DESTFLAGHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 5 | ||
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pDEST_EWSR1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26377 | EWSR1 | Homo sapiens | Ampicillin | PMID:22081015 | Backbone Marker:N/A; Backbone Size:5299; Vector Backbone:pFRT_DESTFLAGHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 3 | ||
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pF149 pSOD1G85RAcGFP1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_26410 | SOD1 | Homo sapiens | Kanamycin | PMID:20221404 | The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 85 of the cDNA from GGC to CGC, resulting in the G85R mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5’ of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein. | Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | G85R | Addgene | 2026-10-31 02:08:12 | 7 |
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pDEST_TO_EWSR1 Resource Report Resource Website |
RRID:Addgene_26376 | EWSR1 | Homo sapiens | Ampicillin | PMID:22081015 | Backbone Marker:N/A; Backbone Size:5421; Vector Backbone:pFRT_TO_DESTFLAGHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:12 | 0 | ||
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pENTR4_EWSR1_G2A_R650H Resource Report Resource Website |
RRID:Addgene_26372 | EWSR1 | Homo sapiens | Kanamycin | PMID:22081015 | Backbone Marker:Invitrogen; Backbone Size:2278; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | R650H | Addgene | 2026-10-31 02:08:12 | 0 | |
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pBCN27-Pmyo-2::GFP::unc-54_3'UTR Resource Report Resource Website |
RRID:Addgene_26347 | Pmyo-2::GFP::unc-54_3'UTR | Caenorhabditis elegans | Ampicillin | PMID:20729840 | The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected. Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus. | Backbone Size:10813; Vector Backbone:pBCN27-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-10-31 02:08:11 | 0 |
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