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Species: Mus musculus
Genetic Insert: Long chain acyl-CoA dehydrogenase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203611
Proper citation: RRID:Addgene_24488 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8863834
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C.
Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24498 Copy
Species: Mus musculus
Genetic Insert: Long chain acyl-CoA dehydrogenase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4400; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20203611
Proper citation: RRID:Addgene_24490 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5."
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24501 Copy
Species: Mus musculus
Genetic Insert: Gq alpha
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline
Defining Citation: PMID:8387644
Comments: This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio.
Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling.
Please see supplemental document for G-protein chimera user manual.
Proper citation: RRID:Addgene_24500 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19966808
Proper citation: RRID:Addgene_24560 Copy
Species: Mus musculus
Genetic Insert: BAF170
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pRRL-sin18PPT CAG-ires-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19279220
Proper citation: RRID:Addgene_24562 Copy
Species: Mus musculus
Genetic Insert: BAF155
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pRRL-sin18PPT CAG-ires-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19279220
Proper citation: RRID:Addgene_24561 Copy
Species: Mus musculus
Genetic Insert: Fli-1
Vector Backbone Description: Backbone Size:6056; Vector Backbone:MigR1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17586726
Comments: Fli-I cloned does not include exon 1. BamHI site (located position 468 of NM_008026) ligated with BglII.
Proper citation: RRID:Addgene_24668 Copy
Species: Mus musculus
Genetic Insert: Par6
Vector Backbone Description: Backbone Size:10400; Vector Backbone:pMEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15761148
Proper citation: RRID:Addgene_24648 Copy
Species: Mus musculus
Genetic Insert: p45 NFE2
Vector Backbone Description: Backbone Size:5800; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8078893
Proper citation: RRID:Addgene_24656 Copy
Species: Mus musculus
Genetic Insert: mFar1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:20111002
Comments: EJ Steen et al. Nature 2009 10.1038/nature08721
Proper citation: RRID:Addgene_24629 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
Clinical, molecular, and genotype-phenotype correlation studies from 25 cases of oral-facial-digital syndrome type 1: a French and Belgian collaborative study.
Thauvin-Robinet C, Cossée M, Cormier-Daire V, Van Maldergem L, Toutain A, Alembik Y, Bieth E, Layet V, Parent P, David A, Goldenberg A, Mortier G, Héron D, Sagot P, Bouvier AM, Huet F, Cusin V, Donzel A, Devys D, Teyssier JR, Faivre L.
J Med Genet. 2006 Jan;43(1):54-61.PMID: 16397067
Proper citation: RRID:Addgene_24581 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells.
Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA.
J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504
Proper citation: RRID:Addgene_24580 Copy
Species: Mus musculus
Genetic Insert: Par6c
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17060907
Proper citation: RRID:Addgene_24598 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Size:4873; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
Four novel mutations in the OFD1 (Cxorf5) gene in Finnish patients with oral-facial-digital syndrome 1.
Rakkolainen A, Ala-Mello S, Kristo P, Orpana A, Järvelä I.
J Med Genet. 2002 Apr;39(4):292-6. No abstract available. PMID: 11950863
Proper citation: RRID:Addgene_24575 Copy
Species: Mus musculus
Genetic Insert: Ofd1
Vector Backbone Description: Backbone Size:4873; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20230748
Comments: Mutation reported in:
OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells.
Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA.
J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504
Proper citation: RRID:Addgene_24574 Copy
Species: Mus musculus
Genetic Insert: Zinc Finger Protein 423
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:pMSCV puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20200519
Proper citation: RRID:Addgene_24764 Copy
Species: Mus musculus
Genetic Insert: IL-10 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2B; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10657644
Proper citation: RRID:Addgene_24948 Copy
Species: Mus musculus
Genetic Insert: IL-10 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2B; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10657644
Comments: There are 3 point mutations in the promoter, but they should not affect activity.
Proper citation: RRID:Addgene_24942 Copy
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