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Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Backbone Size:2961; Vector Backbone:pBluescript II; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Do not cut out EB1 using EcoR1 as there is an internal EcoR1 site in the EB1 sequence.
Proper citation: RRID:Addgene_39324 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39285 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39284 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: Use GST reverse primer to verify mutation in nmt1 promoter
Proper citation: RRID:Addgene_39286 Copy
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39292 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Proper citation: RRID:Addgene_39280 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: nmt1 promoter
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9717240
Comments: 7bp deletion (ATATAAA), 2nt mismatch at bp#4267-4268 and 7nt deletion at bp#4269-4275 when compared to EU493333.1. Does not effect function.
Proper citation: RRID:Addgene_39282 Copy
Species: Mus musculus
Genetic Insert: Kaede
Vector Backbone Description: Vector Backbone:pCS2-Kaede; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19740427
Proper citation: RRID:Addgene_39261 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-25-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGGCGAGCTGCACG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39407 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-25-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCAAGATCCGCCACA
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39406 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-23-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCGTCCTTGAAGAAG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39403 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-24-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TTGCCGTCCTCCTTG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39405 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-06-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGCCACCTACG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.6kb and 5.8kb
Proper citation: RRID:Addgene_39368 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-05-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TAGGTGGCATC
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.6kb and 5.8kb
Proper citation: RRID:Addgene_39367 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-22-Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGGTGCCCATCCTGG
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2kb and 5.8kb
Proper citation: RRID:Addgene_39400 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-07-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGCACGCCGTA
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 1.6kb and 5.8kb
Proper citation: RRID:Addgene_39371 Copy
Genetic Insert: burs-mCD8-EGFP-T2A-Gal4
Vector Backbone Description: Backbone Size:8491; Vector Backbone:pC-attB; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22209908
Proper citation: RRID:Addgene_39463 Copy
Genetic Insert: mCD8-D2A-Gal4
Vector Backbone Description: Backbone Size:6392; Vector Backbone:pPac-PL; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22209908
Proper citation: RRID:Addgene_39457 Copy
Genetic Insert: mCD8-F2A-Gal4
Vector Backbone Description: Backbone Size:6392; Vector Backbone:pPac-PL; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22209908
Proper citation: RRID:Addgene_39459 Copy
Species: Homo sapiens
Genetic Insert: eGFP-TALEN-48-Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TTGAAGTCGATGCCCTTCAGC
To reduce the chance of recombination in this plasmid, do not grow in liquid culture for more than 12-14 hours.
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.6kb and 5.8kb
Proper citation: RRID:Addgene_39453 Copy
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