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  • Organism:mus musculus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pcDNA3.1mLCAD_K42A_FLAG
 
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RRID:Addgene_24488 Long chain acyl-CoA dehydrogenase Mus musculus Ampicillin PMID:20203611 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Lys42Ala 2026-08-15 01:12:32 0
qs5
 
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1+ mentions
RRID:Addgene_24498 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8863834 This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gs alpha residues (EYNLV to QYELL). This construct allows some Gs-coupled receptors to stimulate phospholipase C. Contains an internal hemaglutinin epitope tage (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Replacement of the five carboxyl-terminal amino acids of Gq alpha (EYNLV) with those of Gs alpha(QYELL) 2026-08-15 01:12:31 1
pTrcHisB mLCAD (29-430 aa)
 
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RRID:Addgene_24490 Long chain acyl-CoA dehydrogenase Mus musculus Ampicillin PMID:20203611 Backbone Marker:Invitrogen; Backbone Size:4400; Vector Backbone:pTrcHisB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin aa 1-28 deleted encodes active 29-430 2026-08-15 01:12:31 0
qi5
 
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1+ mentions
RRID:Addgene_24501 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8387644 This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF). This construct allows many Gi-coupled receptors to stimulate phospholipase C (PLC). This is the most popular chimera, perhaps because it is easier to talk about coupling to Gi-coupled receptors with "qi5" rather than "qo5" or "qz5." Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Gq alpha with the C-terminal amino acids changed from Gq alpha to Gi alpha residues (EYNLV to DCGLF) 2026-08-15 01:12:31 6
qo5
 
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1+ mentions
RRID:Addgene_24500 Gq alpha Mus musculus Ampicillin and Tetracycline PMID:8387644 This is Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY). Works the same as qi5 but (for unknown reasons) has a slightly lower basal PLC activity. This can increase the signal-to-noise ratio. Contains an internal hemaglutinin epitope tag (DVPDYA) that has no effect on receptor coupling. Please see supplemental document for G-protein chimera user manual. Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline Gq alpha with the C-terminal amino acids changed from Gq alpha to Go alpha residues (EYNLV to GCGLY) 2026-08-15 01:12:33 1
pIRES2-eGFP-Ofd1-Myc
 
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1+ mentions
RRID:Addgene_24560 Ofd1 Mus musculus Kanamycin PMID:19966808 Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:12:32 3
pRRL CAG NFlag BAF170 ires GFP
 
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1+ mentions
RRID:Addgene_24562 BAF170 Mus musculus Ampicillin PMID:19279220 Backbone Size:9200; Vector Backbone:pRRL-sin18PPT CAG-ires-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:12:33 1
pRRL CAGpNFlag BAF155 ires GFP
 
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1+ mentions
RRID:Addgene_24561 BAF155 Mus musculus Ampicillin PMID:19279220 Backbone Size:9200; Vector Backbone:pRRL-sin18PPT CAG-ires-GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:12:32 2
MigR1 Fli-1 GFP
 
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RRID:Addgene_24668 Fli-1 Mus musculus Ampicillin PMID:17586726 Fli-I cloned does not include exon 1. BamHI site (located position 468 of NM_008026) ligated with BglII. Backbone Size:6056; Vector Backbone:MigR1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:12:34 0
pMEP5-Flag Par6 S345A
 
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1+ mentions
RRID:Addgene_24648 Par6 Mus musculus Ampicillin PMID:15761148 Backbone Size:10400; Vector Backbone:pMEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin S345A 2026-08-15 01:12:34 1
2F+NFE2
 
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RRID:Addgene_24656 p45 NFE2 Mus musculus Ampicillin PMID:8078893 Backbone Size:5800; Vector Backbone:pEF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:12:34 0
pKS18
 
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RRID:Addgene_24629 mFar1 Mus musculus Tetracycline PMID:20111002 EJ Steen et al. Nature 2009 10.1038/nature08721 Backbone Size:0; Vector Backbone:pBBR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:12:32 0
pIRES2-eGFP-Ofd1-Myc-G139S
 
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RRID:Addgene_24581 Ofd1 Mus musculus Kanamycin PMID:20230748 Mutation reported in: Clinical, molecular, and genotype-phenotype correlation studies from 25 cases of oral-facial-digital syndrome type 1: a French and Belgian collaborative study. Thauvin-Robinet C, Cossée M, Cormier-Daire V, Van Maldergem L, Toutain A, Alembik Y, Bieth E, Layet V, Parent P, David A, Goldenberg A, Mortier G, Héron D, Sagot P, Bouvier AM, Huet F, Cusin V, Donzel A, Devys D, Teyssier JR, Faivre L. J Med Genet. 2006 Jan;43(1):54-61.PMID: 16397067 Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Glycine 139 changed to serine (codon GGT to AGT) 2026-08-15 01:12:32 0
pIRES2-eGFP-Ofd1-Myc-S75F
 
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RRID:Addgene_24580 Ofd1 Mus musculus Kanamycin PMID:20230748 Mutation reported in: OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells. Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA. J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504 Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pIRES2-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin serine 75 changed to phenylalanine (codon TCT changed to TTT) 2026-08-15 01:12:33 0
MSCV-Flag-mPar6aK19A
 
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RRID:Addgene_24598 Par6c Mus musculus Ampicillin PMID:17060907 Backbone Marker:Clontech; Backbone Size:8000; Vector Backbone:pMSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin K19A 2026-08-15 01:12:32 0
pFloxin-IRES-Ofd1-Myc-A80T
 
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RRID:Addgene_24575 Ofd1 Mus musculus Ampicillin PMID:20230748 Mutation reported in: Four novel mutations in the OFD1 (Cxorf5) gene in Finnish patients with oral-facial-digital syndrome 1. Rakkolainen A, Ala-Mello S, Kristo P, Orpana A, Järvelä I. J Med Genet. 2002 Apr;39(4):292-6. No abstract available. PMID: 11950863 Backbone Size:4873; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin Alanine 80 changed to threonine (codon GCA to ACA) 2026-08-15 01:12:32 0
pFloxin-IRES-Ofd1-Myc-S75F
 
Resource Report
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RRID:Addgene_24574 Ofd1 Mus musculus Ampicillin PMID:20230748 Mutation reported in: OFD1, the gene mutated in oral-facial-digital syndrome type 1, is expressed in the metanephros and in human embryonic renal mesenchymal cells. Romio L, Wright V, Price K, Winyard PJ, Donnai D, Porteous ME, Franco B, Giorgio G, Malcolm S, Woolf AS, Feather SA. J Am Soc Nephrol. 2003 Mar;14(3):680-9.PMID: 12595504 Backbone Size:4873; Vector Backbone:pBluescript; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin serine 75 changed to phenylalanine (codon TCT changed to TTT) 2026-08-15 01:12:33 0
pMSCVFLAG-ZFP423
 
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1+ mentions
RRID:Addgene_24764 Zinc Finger Protein 423 Mus musculus Ampicillin PMID:20200519 Backbone Marker:Clontech; Backbone Size:6295; Vector Backbone:pMSCV puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:12:34 5
pGL2B -55/+64
 
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RRID:Addgene_24948 IL-10 promoter Mus musculus Ampicillin PMID:10657644 Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2B; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:12:37 0
pGL2B -1538/+64
 
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1+ mentions
RRID:Addgene_24942 IL-10 promoter Mus musculus Ampicillin PMID:10657644 There are 3 point mutations in the promoter, but they should not affect activity. Backbone Marker:Promega; Backbone Size:5597; Vector Backbone:pGL2B; Vector Types:Luciferase; Bacterial Resistance:Ampicillin 2026-08-15 01:12:37 4

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