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Species: Other
Genetic Insert: 2paLINX
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31501546
Proper citation: RRID:Addgene_129655 Copy
Species: Other
Genetic Insert: iRFP-Candida albicans codon-optimized
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:2700; Vector Backbone:pUC57-simple; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28847848
Proper citation: RRID:Addgene_129731 Copy
Species: Other
Genetic Insert: AmiE I122L
Vector Backbone Description: Backbone Size:3583; Vector Backbone:pAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31400199
Proper citation: RRID:Addgene_129792 Copy
Species: Other
Genetic Insert: AmiE I38V
Vector Backbone Description: Backbone Size:3583; Vector Backbone:pAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31400199
Proper citation: RRID:Addgene_129791 Copy
Species: Other
Genetic Insert: CTAG_NLS-SV40
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136108 Copy
Species: Other
Genetic Insert: PROM_35S (CaMV)
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136111 Copy
Species: Other
Genetic Insert: PROM_35Sx2 (CaMV)
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136112 Copy
Species: Other
Genetic Insert: 3TERM_Nos-35S (A. tumefaciens, CaMV)
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Plant Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.29.971002v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_136116 Copy
Species: Other
Genetic Insert: ICP0
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting mCherry for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22976303
Proper citation: RRID:Addgene_134560 Copy
Species: Other
Genetic Insert: ICP0
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting mCherry for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22976303
Comments: Cherry-ICP0FxE-C3 contains a V886A difference compared to the reference sequence. The functional outcome is unknown but this enzyme is catalytically inert as designed.
Proper citation: RRID:Addgene_134562 Copy
Species: Other
Genetic Insert: YFP-ICP0
Vector Backbone Description: Vector Backbone:pLU; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22976303
Proper citation: RRID:Addgene_134563 Copy
Species: Other
Genetic Insert: YFP-ICP0
Vector Backbone Description: Vector Backbone:pLU; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22976303
Comments: pLU-YFP-ICP0FxE contains A514P and V886A differences compared to YP_009137074.1. The functional consequences of these are unknown but the protein is catalytically inert by design.
Proper citation: RRID:Addgene_134564 Copy
Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting mCherry for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134567 Copy
Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134568 Copy
Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting CFP for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134569 Copy
Species: Other
Genetic Insert: mdlC (benzylformate decarboxylase) C -terminal his tag
Vector Backbone Description: Backbone Marker:None; Backbone Size:2100; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:32441815
Comments: Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_134590 Copy
Species: Other
Genetic Insert: P-rhaP-BAD-malE
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18194555
Proper citation: RRID:Addgene_135084 Copy
Species: Other
Genetic Insert: LacZ
Vector Backbone Description: Vector Backbone:pJOE773 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19789867
Proper citation: RRID:Addgene_135087 Copy
Species: Other
Genetic Insert: hcRed-V5-miniTurbo
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135237 Copy
Species: Other
Genetic Insert: hcRed-V5-TurboID
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9014; Vector Backbone:pDest667; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_135238 Copy
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