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Species: Pyrearinus termitilluminans
Genetic Insert: E-Luc
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34520169
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.05.26.443018v2 to read the preprint on bioRxiv.
Proper citation: RRID:Addgene_170873 Copy
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCS2+ (w/ GFP); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Linearize with Not1 or Asp718 and transcribe with SP6.
Notes for Use:
For making carboxy-terminal tagged GFP fusion protein in vector CS2+.
Made by: Jeff Miller.
Proper citation: RRID:Addgene_17097 Copy
Vector Backbone Description: Backbone Size:4103; Vector Backbone:pCS2+ (modified); Vector Types:xenopus/mammalian/avian/zebrafish; Bacterial Resistance:Ampicillin
Comments: pCS2+ has been modified by the deletion of the AC preceeding the T7 promoter addition of the sequence TTCTGCAGCCC immediately following the XbaI site. The PstI site added to CS2+ is encompassed in the sequence (CTGCAG).
Modifications were made by Robert Davis of Marc Kirshner's lab to improve the efficiency of the transcription from the T7 promoter.
Proper citation: RRID:Addgene_17095 Copy
Vector Backbone Description: Backbone Size:5090; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: See Fire Lab Vector Kit Documentation 2005. Fire Lab Miniprep Number pPD158.87, Ligation number L5767.
Proper citation: RRID:Addgene_1709 Copy
Vector Backbone Description: Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33852895
Proper citation: RRID:Addgene_171048 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Vector Backbone:puc19; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29891558
Proper citation: RRID:Addgene_171108 Copy
Genetic Insert: Gag-spyCas9
Vector Backbone Description: Backbone Size:4718; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34077734
Proper citation: RRID:Addgene_171060 Copy
Species: Mus musculus
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34143204
Proper citation: RRID:Addgene_171101 Copy
Species: Rattus norvegicus
Genetic Insert: jGCaMP7b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6296; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34177468
Proper citation: RRID:Addgene_171118 Copy
Vector Backbone Description: Vector Backbone:pISV-DsRed1; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_171024 Copy
Species: Homo sapiens
Genetic Insert: U6:sgRNA_Puro-T2A-BFP
Vector Backbone Description: Backbone Size:9000; Vector Backbone:pCRISPRia-v2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34414886
Proper citation: RRID:Addgene_171011 Copy
Species: Mus musculus
Genetic Insert: hSpCas9
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34143204
Proper citation: RRID:Addgene_171099 Copy
Genetic Insert: GCaMP6s
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_171156 Copy
Genetic Insert: ChroME2f-P2A-H2B-mRuby3
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35120626
Proper citation: RRID:Addgene_171150 Copy
Species: Homo sapiens
Genetic Insert: 2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1(+)-kozakHAHA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: For cloning purpose, an "A" nucleotide was inserted right after BamHI site in the vector backbone by DNA mutagensis to change the open reading frame.
Proper citation: RRID:Addgene_171237 Copy
Species: Homo sapiens
Genetic Insert: 2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1(+)-kozakHAHA; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: For cloning purpose, an "A" nucleotide was inserted right after BamHI site in the vector backbone by DNA mutagensis to change the open reading frame.
Proper citation: RRID:Addgene_171236 Copy
Species: Homo sapiens
Genetic Insert: 3x-Flag-tagged human PP1 alpha catalytic subunit
Vector Backbone Description: Backbone Marker:Sigma-Aldrich; Backbone Size:6400; Vector Backbone:p3XFLAG-CMV-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26636645
Comments: The construction is based on PCR amplification using Pfu DNA polymerase.
Proper citation: RRID:Addgene_171267 Copy
Species: Synthetic
Genetic Insert: J1-BBa_J23117-sfGFP
Vector Backbone Description: Backbone Marker:Herbert Schweizer; Backbone Size:4569; Vector Backbone:pUC18TminiTn7T-Gm; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Gentamicin
Defining Citation: PMID:33930546
Comments: Alternative names: pCDP009, pUC18TminiTn7T-Gm_J1-BBa_J23117-sfGFP_Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A/S101A)
Used for conjugation, with pTNS1 and pRK2013 helper plasmids, to integrate J1-BBa_J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) cassettes into gram-negative bacteria.
Point mutation in the FRT site was observed and remained applicable with counterselection by pCK255
Proper citation: RRID:Addgene_171139 Copy
Species: Synthetic
Genetic Insert: humanized RfxCas13d
Vector Backbone Description: Vector Backbone:pCX539; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33941935
Proper citation: RRID:Addgene_171380 Copy
Species: Mus musculus
Genetic Insert: STIM1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1 myc his; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327260
Comments: Mouse Stim1 (BglII/NotI, NotI is treated by Klenow) was subcloned into BamHI/XbaI (XbaI is treated by Klenow) of pcDNA3.1myc-His vector.
Proper citation: RRID:Addgene_17732 Copy
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