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Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (N728A)
Vector Backbone Description: Backbone Size:6526; Vector Backbone:pNOTAG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15155912
Proper citation: RRID:Addgene_1244 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ydj1
Vector Backbone Description: Backbone Size:3654; Vector Backbone:pJC25; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9674429
Proper citation: RRID:Addgene_1245 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLPo
Vector Backbone Description: Backbone Marker:Karl Deisseroth (pAAV-Ef1a-DIO-hChR2(E123T/T159C)-mCherry, Addgene 35510); Backbone Size:5603; Vector Backbone:pAAV-Ef1a-DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30773318
Proper citation: RRID:Addgene_124641 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ssa1
Vector Backbone Description: Backbone Size:8700; Vector Backbone:pUG2; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9674429
Proper citation: RRID:Addgene_1247 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (K218T)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC45S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:9674429
Comments: Addgene comment: Please note that the plasmid backbone does not match the pJC45 backbone. The depositor described this plasmid as having a "pJC45S" backbone. Full plasmid sequencing shows features similar to other yeast expression vectors. The lab is closed so were were unable to ask if this difference was intended.
Proper citation: RRID:Addgene_1250 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (K620T)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC45S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:9674429
Proper citation: RRID:Addgene_1251 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-0; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25712101
Proper citation: RRID:Addgene_125211 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Marker:Koen Venken; Vector Backbone:pBS-KS-attB-1-2-PT-SA-SD-2; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25712101
Proper citation: RRID:Addgene_125213 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 4 x upstream activating sequence (UAS)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:Unknown; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31092928
Proper citation: RRID:Addgene_125150 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pTDH3-GFPdropout-tTDH1
Vector Backbone Description: Backbone Marker:John Dueber; Backbone Size:1662; Vector Backbone:pYTK001; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30939239
Proper citation: RRID:Addgene_125754 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Multiple cloning site in His3 Ura3 reporter plasmid
Vector Backbone Description: Backbone Size:5790; Vector Backbone:pH3U3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16041365
Comments: Please note that the cloning information for this plasmid has been corrected as of 1/14/09. The 5' cloning site was changed from MluI to NotI.
Proper citation: RRID:Addgene_12609 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: Contains the coding sequence for L-homoserine-O-acetyltransferase.
cerMET2 has A78T and G298E variants compared to the NCBI reference [NP_014122.1]. The plasmid has been used for complementation analyses and should function as described.
Proper citation: RRID:Addgene_107435 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for siroheme synthase
Proper citation: RRID:Addgene_107439 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for the beta subunit of sulfite reductase
Construct was not functional in complementation analysis, but this may be due to tags rather than mutations in the coding sequence. Use with caution.
Proper citation: RRID:Addgene_107437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET17
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for homocysteine synthase
Proper citation: RRID:Addgene_107443 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MET16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5886; Vector Backbone:pYES2.1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29326101
Comments: contains the coding sequence for 3'-phosphoadenylsulfate reductase
Proper citation: RRID:Addgene_107442 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: X-3 site gRNA expression cassettes
Vector Backbone Description: Backbone Size:5856; Vector Backbone:pYES2; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30199094
Comments: pYES2-gRNA-hyg-MCS is an E. coli-S. cerevisiae shuttle plasmid which harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site gRNA (HXK1 gene).
However, it can function as an empty gRNA expression backbone plasmid because the existing gRNA expression cassette can be easily replaced with conventional restriction enzyme digestion-ligation method. Moreover, multiple gRNA expression cassettes can be loaded in the MCS.
Proper citation: RRID:Addgene_107734 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y, gRNA-ADE2.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:5462; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107930 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:6130; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107919 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: gRNA-CAN1.Y
Vector Backbone Description: Backbone Marker:George Church (Addgene plasmid # 43803); Backbone Size:6356; Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25743786
Proper citation: RRID:Addgene_107918 Copy
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