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Species: Homo sapiens
Genetic Insert: ss-cfSGFP2 (cysteine-free SGFP2)
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4105; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22649538
Comments: This plasmid is used for expression of cfSGFP2 in the ER. The cleaved signal sequence of α1-antitrypsin (M1-K34) was fused to the N-terminus of SGFP2 using BglII and EcoRI sites. The fragment M1-A24 should be removed cotranslationally after translocation. The long flanking sequence E25-K34 of α1-antitrypsin was included to ensure access to signal peptidase
Proper citation: RRID:Addgene_37535 Copy
Species: Mus musculus
Genetic Insert: Smo
Vector Backbone Description: Vector Backbone:pEG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10984056
Comments: Addgene NGS results identified a D54N variant compared to the NCBI reference [EDL13766.1].
Proper citation: RRID:Addgene_37674 Copy
Species: Mycobacterium tuberculosis
Genetic Insert: Rv2416c
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22547814
Proper citation: RRID:Addgene_37649 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Vector Backbone:pShuttle; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22483942
Proper citation: RRID:Addgene_37829 Copy
Species: bacteria
Genetic Insert: Promotorless gfp reporter gene
Vector Backbone Description: Vector Backbone:pBBR1; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11059491
Comments: Please refer to the attached table for a complete list of restriction sites in the MCS.
This plasmid has the PstI site.
Proper citation: RRID:Addgene_37819 Copy
Species: bacteria
Genetic Insert: Promotorless gfp reporter gene
Vector Backbone Description: Vector Backbone:pVS1/p15a; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11059491
Comments: Please refer to the attached table for a complete list of restriction sites in the MCS.
This plasmid has the PstI, SalI, SmaI and XbaI sites.
Proper citation: RRID:Addgene_37816 Copy
Species: Homo sapiens
Genetic Insert: EB1
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22483942
Comments: This construct is resistant to shRNA #3.
EB1 was inserted into pEGFP-N1 via the SacI/SmaI restriction sites. The second EGFP, was then inserted via the SmaI/AgeI sites.
Proper citation: RRID:Addgene_37925 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Operon: cfp upstream of yfp
Chromosomal integration at grtAB and/or IntS: KanRnoFRT, pLlacO-1:T710 CFP::T710 YFP::Asp
Proper citation: RRID:Addgene_37987 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Operon: yfp upstream of cfp
Chromosomal integration at galM and/or galK: KanRnoFRT, pLlacO-1:T710 YFP::T710 CFP::Asp
Proper citation: RRID:Addgene_37988 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Non-coregulated, Non-clustered
Chromosomal integration at gtrAB and/or IntS: KanRnoFRT, pLlacO-1:T710 CFP::T1
Chromosomal integration at galM and/or galK: T1::pLtetO-1:T710 YFP::Asp
Proper citation: RRID:Addgene_37983 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Non-coregulated, codirectional: cfp upstream of yfp
Chromosomal integration at gtrAB and/or IntS: KanRnoFRT, pLlacO-1:T710 CFP::T1::pLtetO-1:T710 YFP::Asp
Proper citation: RRID:Addgene_37985 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23222655
Comments: Strain description: Non-clustered
Chromosomal integration at gtrAB and/or IntS: KanRnoFRT, pLlacO-1:T710 CFP::T1
Chromosomal integration at galM and/or galK: T1::pLlacO-1:T710 YFP::Asp
Proper citation: RRID:Addgene_37982 Copy
Species: Begomovirus
Genetic Insert: pJRTCLCrVA.009
Vector Backbone Description: Backbone Marker:Cambia.org; Backbone Size:8935; Vector Backbone:pCAMBIA 1300; Vector Types:Agrobacterium Binary; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22853641
Proper citation: RRID:Addgene_37972 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38012 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38010 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.
Proper citation: RRID:Addgene_38011 Copy
Species: Mus musculus
Genetic Insert: Clathrin, light polypeptide (LCa)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4000; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21552254
Comments: Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function. It is also missing its stop codon, so the peptide "PGPGSTGSR*" from the backbone is appended to the C-terminus of the CLC. The affect of this peptide on protein function is unknown.
Proper citation: RRID:Addgene_38009 Copy
Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22722250
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38001 Copy
Species: Homo sapiens
Genetic Insert: FKBP1A
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pmRFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22722250
Comments: Compared to GenBank reference sequence NM_019892, the INPP5E insert in this plasmid contains a C641A mutation. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_38000 Copy
Species: Homo sapiens
Genetic Insert: MITF-A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22692423
Proper citation: RRID:Addgene_38132 Copy
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