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Species: Homo sapiens
Genetic Insert: ubc44.001.167
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 1YH2 http://www.thesgc.org/structures/1YH2/
Proper citation: RRID:Addgene_25468 Copy
Species: Homo sapiens
Genetic Insert: GNPNAT1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2HUZ, 2O28, https://www.rcsb.org/structure/2O28
SGC links:
https://www.thesgc.org/structures/2HUZ
https://www.thesgc.org/structures/2O28
Proper citation: RRID:Addgene_25501 Copy
Species: Homo sapiens
Genetic Insert: ubh26.019.099
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 1YQB http://www.thesgc.org/structures/1YQB/
Proper citation: RRID:Addgene_25467 Copy
Species: Mus musculus
Genetic Insert: BAF53(b+a)
Vector Backbone Description: Backbone Size:6000; Vector Backbone:Beta-actin/CMV-IRES-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17920018
Proper citation: RRID:Addgene_25462 Copy
Species: Mus musculus
Genetic Insert: BAF53(a+b)
Vector Backbone Description: Backbone Marker:home made; Backbone Size:6000; Vector Backbone:Beta-actin/CMV-IRES-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17920018
Proper citation: RRID:Addgene_25461 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Ubc13
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX4T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16980971
Proper citation: RRID:Addgene_25464 Copy
Species: Mus musculus
Genetic Insert: BAF53a
Vector Backbone Description: Backbone Marker:home made; Backbone Size:6000; Vector Backbone:Beta-actin/CMV-IRES-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17920018
Proper citation: RRID:Addgene_25460 Copy
Species: Mus musculus
Genetic Insert: mCherry-miR155-lacZalpha
Vector Backbone Description: Backbone Marker:unpublished; Backbone Size:6700; Vector Backbone:pMA2767; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19655272
Proper citation: RRID:Addgene_25439 Copy
Species: Synthetic
Genetic Insert: reverse tetracycline-regulated transactivator Advanced
Vector Backbone Description: Backbone Marker:unpublished; Backbone Size:7209; Vector Backbone:pMA2636rc; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19655272
Proper citation: RRID:Addgene_25434 Copy
Species: Homo sapiens
Genetic Insert: Myo D Promoter
Vector Backbone Description: Backbone Marker:Fire et al., 1990; Backbone Size:6110; Vector Backbone:pPD46.21; Vector Types:Mammalian Expression, Reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7720572
Comments: A SalI-XhoI fragment containing the 2.5 kb promoter fragment was cloned into the SalI site of pPD46.21, destroying the XhoI site. The F3 fragment is cloned in reverse orientation.
258 refers to the core enhancer, which is present in this construct, upstream of the 2.5 kb MyoD promoter
Proper citation: RRID:Addgene_25430 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Synthetic Green Fluorescent Protein - VRG4
Vector Backbone Description: Backbone Size:5500; Vector Backbone:YCPlac33; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16699524
Proper citation: RRID:Addgene_25447 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sec13-Green Fluorescent Protein
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pUC19-URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10189369
Proper citation: RRID:Addgene_25445 Copy
Species: Rattus norvegicus
Genetic Insert: Kal9
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEAK-His-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777487
Comments: There are 2 mutations in the sequence, T46S and N62Y. These sequencing errors are the result of mistakes in initial submission to GenBank by the depositing lab. The sequence should be correct.
Proper citation: RRID:Addgene_25441 Copy
Species: Mus musculus
Genetic Insert: Diap3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:EF-BOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11035012
Comments: The Q1091P mutation in the DAD region was not intentional and may have been a cloning error.
Proper citation: RRID:Addgene_25411 Copy
Species: Homo sapiens
Genetic Insert: MAK3
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2OB0 http://www.thesgc.org/structures/2OB0/
Proper citation: RRID:Addgene_25499 Copy
Species: Homo sapiens
Genetic Insert: GAMT
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 1ZX0 http://www.thesgc.org/structures/1ZX0/
Proper citation: RRID:Addgene_25494 Copy
Species: Homo sapiens
Genetic Insert: SRM
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB:
http://www.thesgc.org/structures/2O05/
http://www.thesgc.org/structures/2O06/
http://www.thesgc.org/structures/1ZDZ/
Proper citation: RRID:Addgene_25490 Copy
Species: Homo sapiens
Genetic Insert: SAT
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2FXF http://www.thesgc.org/structures/2FXF/
Proper citation: RRID:Addgene_25493 Copy
Species: Homo sapiens
Genetic Insert: SULT1C1
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2ETG
http://www.thesgc.org/structures/2ETG/
Proper citation: RRID:Addgene_25492 Copy
Species: Homo sapiens
Genetic Insert: Myo D Promoter + F3
Vector Backbone Description: Backbone Marker:Mellon Lab; Backbone Size:4100; Vector Backbone:pCATdeltaEH; Vector Types:Mammalian Expression, Reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1315077
Comments: Fragment 3 was cloned into the XbaI site of -2.5CAT by digesting chMD-13 with NotI and EcoRI, and blunt-end-ligating the fragment into the unique XbaI site.
The -2.5 promoter fragment can be removed by digestion with SalI or NotI and XhoI. The resulting fragment is approximately 6.8kb.
All tk sequences were removed from the CAT vector in the construction of -2.5kb.
Between the enhancer and promoter is a portion of pBluescript polylinker that includes BamHI, SpeI and SmaI sites, none of which are unique.
Proper citation: RRID:Addgene_25425 Copy
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