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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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mCherry-miniSOG-Keratin-17 Resource Report Resource Website |
RRID:Addgene_55091 | Keratin | Homo sapiens | Kanamycin | PMID:21483721 | . Excitation = 448 / 473; Emission = 500 / 528 | Backbone Size:5500; Vector Backbone:mCherry-miniSOG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | V282L | 2026-08-15 01:16:46 | 0 |
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mCherry-Talin-N-10 Resource Report Resource Website 1+ mentions |
RRID:Addgene_55139 | Talin | Mus musculus | Kanamycin | PMID:22751201 | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 2 | |
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mCherry-SSTR3-N-17 Resource Report Resource Website |
RRID:Addgene_55135 | SSTR3 | Mus musculus | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 0 | ||
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mCherry-Smoothened1-N-18 Resource Report Resource Website 1+ mentions |
RRID:Addgene_55134 | Smoothened1 | Mus musculus | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 2 | ||
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mCherry-miniSOG-Vimentin-7 Resource Report Resource Website |
RRID:Addgene_55099 | NM_003380.3 | Homo sapiens | Kanamycin | PMID:21483721 | . Excitation = 448 / 473; Emission = 500 / 528 | Backbone Size:5500; Vector Backbone:mCherry-miniSOG; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:46 | 0 | |
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mCherry-Sequestosome1-N-18 Resource Report Resource Website 1+ mentions |
RRID:Addgene_55132 | Sequestosome1 | Homo sapiens | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 6 | ||
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mCherry-TBRII-C-18 Resource Report Resource Website |
RRID:Addgene_55141 | TBRII | Homo sapiens | Kanamycin | TBRII contains the H385N mutation . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | H385N | 2026-08-15 01:16:47 | 0 | |
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mCherry-Talin-R-15 Resource Report Resource Website |
RRID:Addgene_55140 | Talin R | Mus musculus | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Talin Rod Domain | 2026-08-15 01:16:47 | 0 | |
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mCherry-TOMM20-N-10 Resource Report Resource Website 10+ mentions |
RRID:Addgene_55146 | TOMM20 | Homo sapiens | Kanamycin | Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 28 | ||
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mCherry-TFR-20 Resource Report Resource Website 10+ mentions |
RRID:Addgene_55144 | TFR | Homo sapiens | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 13 | ||
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mCherry-Tensin1-C-14 Resource Report Resource Website 1+ mentions |
RRID:Addgene_55143 | Tensin1 | Homo sapiens | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 1 | ||
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mCherry-VASP-N-10 Resource Report Resource Website |
RRID:Addgene_55153 | VASP | Mus musculus | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | A209T in VASP | 2026-08-15 01:16:47 | 0 | |
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mCherry-UbiquitinC-N-18 Resource Report Resource Website |
RRID:Addgene_55150 | UbiquitinC | Homo sapiens | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | aa 2-76 of NP_003324.1 | 2026-08-15 01:16:47 | 0 | |
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mCherry-WASH1-C-18 Resource Report Resource Website 1+ mentions |
RRID:Addgene_55162 | WASH1 | Mus musculus | Kanamycin | WASH = WASH1. Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 1 | ||
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mCherry-WASF2-N Resource Report Resource Website |
RRID:Addgene_55161 | WASF2 | Mus musculus | Kanamycin | . Excitation = 587; Emission = 610 | Backbone Size:4750; Vector Backbone:mCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 0 | ||
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pET StrepII-StrepII TEV LIC cloning vector (2RRT) Resource Report Resource Website 1+ mentions |
RRID:Addgene_55215 | Ampicillin | This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z). 2RRT has a TEV-cleavable N-terminal StrepII-StrepII tag. To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'-TACTTCCAATCCAATGCA-3' Reverse - 5'-TTATCCACTTCCAATGTTATTA-3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. | Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:48 | 1 | ||||
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His10-tag Resource Report Resource Website |
RRID:Addgene_55179 | His10 | Synthetic | Chloramphenicol | PMID:24295448 | For sequencing of insert, use the following primers: fwd: TGCCACCTGACGTCTAAG rev: ATTACCGCCTTTGAGTGA | Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:16:47 | 0 | |
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pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69) Resource Report Resource Website |
RRID:Addgene_55212 | Ampicillin | This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z). 2-HpI-69 has a TEV-cleavable N-terminal His6-High pI protein fusion tag. The high pI can improve solubility of your target protein. To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'-TACTTCCAATCCAATGCA-3' Reverse - 5'-TTATCCACTTCCAATGTTATTA-3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. | Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:48 | 0 | ||||
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pET His6 High pI protein 68 TEV LIC cloning vector (2-HpI-68) Resource Report Resource Website 1+ mentions |
RRID:Addgene_55211 | Ampicillin | This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z). 2-HpI-68 has a TEV-cleavable N-terminal His6-High pI protein fusion tag. The high pI can improve solubility of your target protein.To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'-TACTTCCAATCCAATGCA-3' Reverse - 5'-TTATCCACTTCCAATGTTATTA-3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. | Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:48 | 1 | ||||
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pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B) Resource Report Resource Website 10+ mentions |
RRID:Addgene_55219 | Ampicillin | PMID:28668116 | In order to increase the efficiency of subcloning with the Series-11 Macrobac plasmids we developed a new strategy the uses LIC (Ligation Independent Cloning). The problem with the Series-11 ligation mediated subcloning was that when the plasmid size approached or exceeded 20 kb we had to screen many colonies to find a positive. We developed a strategy to use LIC for subcloning. Because this method uses no ligase, the cloning background associated with re-ligation of the empty plasmid are eliminated. 438-B has a TEV cleavable His6 at the N-terminus. The 438-B vector use the LICv1 Forward and Reverse primers. LICv1Forward - 5'-TACTTCCAATCCAATGCA-3' LICv1 Reverse - 5'-TTATCCACTTCCAATGTTATTA-3' As the target plasmid size gets >20kb you may have to increase the amount of DNA you anneal and/or transform. We have found this method gives no background colonies at all and 100% of the colonies we pick are positive. The cells we transform into are XL1Blues with a competency ~6x10^7cfu/ul. For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:48 | 14 |
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