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Species: Other
Genetic Insert: pLac-Mango
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37699423
Proper citation: RRID:Addgene_167246 Copy
Species: Other
Genetic Insert: pT7-LacI
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37699423
Proper citation: RRID:Addgene_167248 Copy
Species: Other
Genetic Insert: pPbr-AKSIRV
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37699423
Proper citation: RRID:Addgene_167230 Copy
Species: Other
Genetic Insert: pAKSIRV-Mango
Vector Backbone Description: Vector Backbone:pJL1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37699423
Proper citation: RRID:Addgene_167250 Copy
Species: Other
Genetic Insert: Plastid transit peptide fused sfGFP
Vector Backbone Description: Vector Backbone:pGWB508; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36705704
Proper citation: RRID:Addgene_213863 Copy
Species: Other
Genetic Insert: mCherry-sfGFP
Vector Backbone Description: Vector Backbone:pGWB602; Vector Types:Bacterial Expression, Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36705704
Proper citation: RRID:Addgene_213860 Copy
Species: Other
Genetic Insert: ER-TurboID-V5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214453 Copy
Species: Other
Genetic Insert: PM-miniTurbo-V5
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:9019; Vector Backbone:pDest663; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination; Bacterial Resistance:Ampicillin
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community. These lentiviral gateway destination plasmids were generated, as part of the Kinase-Data and Resource Generating Center (DRGC), using the single fragment or multisite gateway cloning technology. They were used for generating proteomics-based protein-interaction and protein-proximity networks that can be accessed through the kinase-DRGC website https://darkkinome.org/. Each plasmid has either an N- or C-terminal fusion protein (Flag/ V5/V5-miniTurbo/V5-TurboID/miniTurbo-V5/TurboID-V5) tagged understudied kinase driven under either a CMV or Ubiquitin (UBC) promoter. All inserts in the pENTR plasmids used to generate the deposited destination plasmids were end-sequenced and insert size validated prior to multi-site gateway cloning. The combined insert size of the single fragment or three fragment destination plasmids were confirmed by restriction digestion (BsrGI, and EcoRV for pDest667; BsrGI, EcoRV, and BstZ17I for pDest663; and BsrGI for pHAGE) and all plasmids were partially sequenced to ensure in-frame ORFs and fusion tags.
Proper citation: RRID:Addgene_214454 Copy
Species: Other
Genetic Insert: SpCas9_introns_2xNLS-Pea3A(T) term
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37682951
Proper citation: RRID:Addgene_207908 Copy
Species: Other
Genetic Insert: RspA-N
Vector Backbone Description: Vector Backbone:mApple-6xHp; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38949658
Proper citation: RRID:Addgene_214412 Copy
Species: Other
Genetic Insert: RspA-N
Vector Backbone Description: Vector Backbone:NFAST-Halo-ERM; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38949658
Proper citation: RRID:Addgene_214419 Copy
Species: Other
Genetic Insert: pMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
Vector Backbone Description: Vector Backbone:pSUN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37403571
Proper citation: RRID:Addgene_210559 Copy
Species: Other
Genetic Insert: GL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
Vector Backbone Description: Vector Backbone:pSUN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37403571
Proper citation: RRID:Addgene_210555 Copy
Species: Other
Genetic Insert: CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
Vector Backbone Description: Vector Backbone:pSUN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37403571
Proper citation: RRID:Addgene_210550 Copy
Species: Other
Genetic Insert: pU6::AAVS1gRNA::LT2::mCherrySSM
Vector Backbone Description: Vector Backbone:pSUN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37403571
Proper citation: RRID:Addgene_210553 Copy
Species: Other
Genetic Insert: pU6::AAVS1gRNA::LT0::mCherrySSM
Vector Backbone Description: Vector Backbone:pSUN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37403571
Proper citation: RRID:Addgene_210551 Copy
Species: Other
Genetic Insert: pU6::AAVS1gRNA::LT1::mCherrySSM
Vector Backbone Description: Vector Backbone:pSUN; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37403571
Proper citation: RRID:Addgene_210552 Copy
Species: Other
Genetic Insert: mNeonGreen-HMG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5625; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36014487
Proper citation: RRID:Addgene_206872 Copy
Species: Other
Genetic Insert: Firefly luciferase
Vector Backbone Description: Vector Backbone:pRD_RIPE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38375914
Comments: Please visit https://doi.org/10.1101/2023.11.14.567061 for bioRxiv preprint.
Proper citation: RRID:Addgene_214075 Copy
Species: other
Genetic Insert: BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
Vector Backbone Description: Vector Backbone:p15A ; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:38086386
Proper citation: RRID:Addgene_214739 Copy
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