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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A) Resource Report Resource Website 10+ mentions |
RRID:Addgene_55218 | Ampicillin | PMID:28668116 | In order to increase the efficiency of subcloning with the Series-11 Macrobac plasmids we developed a new strategy the uses LIC (Ligation Independent Cloning). The problem with the Series-11 ligation mediated subcloning was that when the plasmid size approached or exceeded 20 kb we had to screen many colonies to find a positive. We developed a strategy to use LIC for subcloning. Because this method uses no ligase, the cloning background associated with re-ligation of the empty plasmid are eliminated. 438-A is an untagged pFastBac LIC Subcloning vector. The 438 vectors use the LICvBac Forward primer and the LICv1 Reverse primer. LICvBacF - 5'-TACTTCCAATCCAATCG-3' (Note: ATG must be added to ORF) LICv1 Reverse - 5'-TTATCCACTTCCAATGTTATTA-3' As the target plasmid size gets >20kb you may have to increase the amount of DNA you anneal and/or transform. We have found this method gives no background colonies at all and 100% of the colonies we pick are positive. The cells we transform into are XL1Blues with a competency ~6x10^7cfu/ul. For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:48 | 24 | |||
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pCoofy48 Resource Report Resource Website |
RRID:Addgene_55186 | Kanamycin | PMID:23410102 | C-terminal tags are separated by a stop codon. Depending on the LP2 primer used in SLIC, the desired C-terminal tag(s) can be fused to the target gene. Use the one of the following linearization primers pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer: LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3' LP2 - select an LP2 primer below depending on which C-terminal tags you want to include fused to your gene of interest: none SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3' 10His- SLIC Primer 5`GAGCATCATCATCATCACCAC 3' StrepOne - SLIC Primer 5`AGCGCTTGGAGCCACCCGCAG 3' S-Tag - SLIC Primer 5`AAAGAAACCGCTGCTGCTAAATTCG 3' CBP - SLIC Primer 5`ATGAAGCGGCGGTGGAAGAAAAAC 3' HPC4 - SLIC Primer 5`GAGGACCAGGTGGACCCCCGG 3' Æ54CPD - SLIC Primer 5`GGCAGCGGCAAGATCCTGCAC 3' Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Novagen; Backbone Size:7266; Vector Backbone:pET, pCoofy3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 0 | |||
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pCoofy44 Resource Report Resource Website |
RRID:Addgene_55185 | Ampicillin | PMID:23410102 | C-terminal tags are separated by a stop codon. Depending on the LP2 primer used in SLIC, the desired C-terminal tag(s) can be fused to the target gene. Use the one of the following linearization primers pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer: LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3' LP2 - select an LP2 primer below depending on which C-terminal tags you want to include fused to your gene of interest: none SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3' 10His- SLIC Primer 5`GAGCATCATCATCATCACCAC 3' StrepOne - SLIC Primer 5`AGCGCTTGGAGCCACCCGCAG 3' S-Tag - SLIC Primer 5`AAAGAAACCGCTGCTGCTAAATTCG 3' HPC4 - SLIC Primer 5`GAGGACCAGGTGGACCCCCGG 3' Æ54CPD - SLIC Primer 5`GGCAGCGGCAAGATCCTGCAC 3' Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Life Technologies; Backbone Size:6383; Vector Backbone:pFastBac; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:47 | 0 | |||
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pCoofy41 Resource Report Resource Website 1+ mentions |
RRID:Addgene_55184 | Ampicillin | PMID:23410102 | C-terminal tags are separated by a stop codon. Depending on the LP2 primer used in SLIC, the desired C-terminal tag(s) can be fused to the target gene Use the one of the following linearization primers pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer: LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3' LP2 - select an LP2 primer below depending on which C-terminal tags you want to include fused to your gene of interest: none SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3' 10His- SLIC Primer 5`GAGCATCATCATCATCACCAC 3' StrepOne - SLIC Primer 5`AGCGCTTGGAGCCACCCGCAG 3' S-Tag - SLIC Primer 5`AAAGAAACCGCTGCTGCTAAATTCG 3' HPC4 - SLIC Primer 5`GAGGACCAGGTGGACCCCCGG 3' Æ54CPD - SLIC Primer 5`GGCAGCGGCAAGATCCTGCAC 3' Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Life Technologies; Backbone Size:6063; Vector Backbone:pFastBac; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:47 | 1 | |||
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cMyc-tag Resource Report Resource Website |
RRID:Addgene_55183 | cMyc tag | Synthetic | Chloramphenicol | PMID:24295448 | For sequencing of insert, use the following primers: fwd: TGCCACCTGACGTCTAAG rev: ATTACCGCCTTTGAGTGA | Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:16:47 | 0 | |
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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3) Resource Report Resource Website |
RRID:Addgene_55223 | Ampicillin | PMID:28668116 | In order to increase the efficiency of subcloning with the Series-11 Macrobac plasmids we developed a new strategy the uses LIC (Ligation Independent Cloning). The problem with the Series-11 ligation mediated subcloning was that when the plasmid size approached or exceeded 20 kb we had to screen many colonies to find a positive. We developed a strategy to use LIC for subcloning. Because this method uses no ligase, the cloning background associated with re-ligation of the empty plasmid are eliminated. 438-SNAP-V3 has a TEV cleavable TwinStrepII-Prescission-SNAPf on the C-terminus. The 438-SNAP-V3 vector use the LICv3 Forward and Reverse primers. LICv3 Forward - 5'-TTTAAGAAGGAGATATAGTTC-3' LICv3 Reverse - 5'-GGATTGGAAGTAGAGGTTCTC-3' As the target plasmid size gets >20kb you may have to increase the amount of DNA you anneal and/or transform. We have found this method gives no background colonies at all and 100% of the colonies we pick are positive. The cells we transform into are XL1Blues with a competency ~6x10^7cfu/ul. For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:48 | 0 | |||
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pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp) Resource Report Resource Website 1+ mentions |
RRID:Addgene_55221 | Ampicillin | PMID:28668116 | In order to increase the efficiency of subcloning with the Series-11 Macrobac plasmids we developed a new strategy the uses LIC (Ligation Independent Cloning). The problem with the Series-11 ligation mediated subcloning was that when the plasmid size approached or exceeded 20 kb we had to screen many colonies to find a positive. We developed a strategy to use LIC for subcloning. Because this method uses no ligase, the cloning background associated with re-ligation of the empty plasmid are eliminated. 438-Rgfp has a TEV cleavable StrepII and msfGFP at the N-terminus. The 438-Rgfp vector use the LICv1 Forward and Reverse primers. LICv1Forward - 5'-TACTTCCAATCCAATGCA-3' LICv1 Reverse - 5'-TTATCCACTTCCAATGTTATTA-3' As the target plasmid size gets >20kb you may have to increase the amount of DNA you anneal and/or transform. We have found this method gives no background colonies at all and 100% of the colonies we pick are positive. The cells we transform into are XL1Blues with a competency ~6x10^7cfu/ul. For more information, please see our website: http://qb3.berkeley.edu/qb3/macrolab/ | Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:48 | 6 | |||
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pCoofy49 Resource Report Resource Website |
RRID:Addgene_55187 | Kanamycin | PMID:23410102 | C-terminal tags are separated by a stop codon. Depending on the LP2 primer used in SLIC, the desired C-terminal tag(s) can be fused to the target gene. Use the one of the following linearization primers pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer: LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3' LP2 - select an LP2 primer below depending on which C-terminal tags you want to include fused to your gene of interest: none SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3' 10His- SLIC Primer 5`GAGCATCATCATCATCACCAC 3' StrepOne - SLIC Primer 5`AGCGCTTGGAGCCACCCGCAG 3' S-Tag - SLIC Primer 5`AAAGAAACCGCTGCTGCTAAATTCG 3' CBP - SLIC Primer 5`ATGAAGCGGCGGTGGAAGAAAAAC 3' HPC4 - SLIC Primer 5`GAGGACCAGGTGGACCCCCGG 3' Æ54CPD - SLIC Primer 5`GGCAGCGGCAAGATCCTGCAC 3' Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. | Backbone Marker:Novagen; Backbone Size:7701; Vector Backbone:pET, pCoofy4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:47 | 0 | |||
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pFusX3-12 Resource Report Resource Website |
RRID:Addgene_63357 | NI NN NG | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:01 | 0 | |
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pFusX3-13 Resource Report Resource Website |
RRID:Addgene_63358 | NI NG NI | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:01 | 0 | |
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pFusX3-8 Resource Report Resource Website |
RRID:Addgene_63353 | NI HD NG | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:01 | 0 | |
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pFusX3-9 Resource Report Resource Website |
RRID:Addgene_63354 | NI NN NI | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:01 | 0 | |
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pFusX3-10 Resource Report Resource Website |
RRID:Addgene_63355 | NI NN HD | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:01 | 0 | |
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pFusX3-11 Resource Report Resource Website |
RRID:Addgene_63356 | NI NN NN | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:01 | 0 | |
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pFusX3-4 Resource Report Resource Website |
RRID:Addgene_63350 | NI NI NG | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:01 | 0 | |
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pFusX3-5 Resource Report Resource Website |
RRID:Addgene_63351 | NI HD NI | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:01 | 0 | |
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pFusX3-56 Resource Report Resource Website |
RRID:Addgene_63401 | NG HD NG | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:02 | 0 | |
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pFusX3-24 Resource Report Resource Website |
RRID:Addgene_63369 | HD HD NG | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:02 | 0 | |
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pFusX3-59 Resource Report Resource Website |
RRID:Addgene_63404 | NG NN NN | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:02 | 0 | |
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pFusX3-55 Resource Report Resource Website |
RRID:Addgene_63400 | NG HD NN | Synthetic | Spectinomycin | PMID:26854857 | Backbone Size:3015; Vector Backbone:FusX3; Vector Types:TALEN; Bacterial Resistance:Spectinomycin | none | 2026-08-15 01:18:02 | 0 |
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