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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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Gi1-CASE Resource Report Resource Website 1+ mentions |
RRID:Addgene_168120 | GNAI1, GNB1, GNG2 | Homo sapiens | Kanamycin | PMID:34516756 | Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint. | Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin | NLuc is inserted at A121/E122 within GNAI1 | 2026-08-15 01:09:50 | 3 |
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Gi2-CASE Resource Report Resource Website 1+ mentions |
RRID:Addgene_168121 | GNAI2, GNB1, GNG2 | Homo sapiens | Kanamycin | PMID:34516756 | Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint. | Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin | NLuc is inserted at C112/E115 within GNAI2 | 2026-08-15 01:09:50 | 1 |
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Go1-CASE Resource Report Resource Website 1+ mentions |
RRID:Addgene_168123 | GNAO1, GNB3, GNG9 | Homo sapiens | Kanamycin | PMID:34516756 | Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint. | Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin | NLuc is inserted at L91/G92 within GNAO1 | 2026-08-15 01:09:50 | 2 |
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pMRX-IPU-GFP-NEK9W967A Resource Report Resource Website |
RRID:Addgene_168271 | NEK9 | Homo sapiens | Ampicillin | PMID:34078910 | Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6100; Vector Backbone:pMRX-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | changed tryptophan 979 to alanine | 2026-08-15 01:09:52 | 0 | |
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pET28a-TEV-U1AF37MF77M Resource Report Resource Website |
RRID:Addgene_168267 | U1A RRM1 crystallization module | Homo sapiens | Kanamycin | PMID:33777416 | Backbone Marker:GenScript; Backbone Size:5400; Vector Backbone:pET28a-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | changed F37M and F77M | 2026-08-15 01:09:52 | 0 | |
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pMRX-IPU-FLAG-NEK9 Resource Report Resource Website |
RRID:Addgene_168269 | NEK9 | Homo sapiens | Ampicillin | PMID:34078910 | Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6100; Vector Backbone:pMRX-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:52 | 0 | ||
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LiPOP1-NanoLuc Resource Report Resource Website |
RRID:Addgene_168265 | MLKL(1-125) | Homo sapiens | Kanamycin | PMID:34540558 | Backbone Marker:Addgene; Backbone Size:4750; Vector Backbone:mCherry2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | H15A, K16A, R17A | 2026-08-15 01:09:52 | 0 | |
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pMEH365 Hspa8 HA Resource Report Resource Website |
RRID:Addgene_168219 | Hspa8 | Homo sapiens | Ampicillin | PMID:27189348 | Backbone Marker:Clontech; Backbone Size:5573; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:52 | 0 | ||
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pMEH60 Lamp2b MS2 HA Resource Report Resource Website |
RRID:Addgene_168209 | Lamp2b | Homo sapiens | Ampicillin | PMID:27189348 | Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:51 | 0 | ||
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pMEH371 CD63 HA Resource Report Resource Website 1+ mentions |
RRID:Addgene_168220 | CD63 | Homo sapiens | Ampicillin | PMID:27189348 | Backbone Marker:Clontech; Backbone Size:5520; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:51 | 1 | ||
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(365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4) Resource Report Resource Website |
RRID:Addgene_168188 | HA-nUbc6E-(EAAAK)4-OGT(4) | Homo sapiens | Ampicillin | PMID:33950562 | Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:51 | 0 | ||
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(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A) Resource Report Resource Website |
RRID:Addgene_168189 | HA-nUbc6E-(EAAAK)4-OGT(4,K852A) | Homo sapiens | Ampicillin | PMID:33950562 | Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:51 | 0 | ||
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(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A) Resource Report Resource Website |
RRID:Addgene_168191 | HA-nLag6-(EAAAK)4-OGT(4,K852A) | Homo sapiens | Ampicillin | PMID:33950562 | Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:51 | 0 | ||
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XE224 human flag-Chibby-pCS3+FLT Resource Report Resource Website |
RRID:Addgene_16824 | flag-Chibby | Homo sapiens | Ampicillin | PMID:12712206 | The full coding sequence as well as 3' UTR of Human Chibby, ~1kb, was amplified by PCR and subcloned into the EcoR1 and Xho1 sites of pCS3+FLT, providing a flag tag. Reference: K. Takemaru et al., Nature 422: 905 (2003) | Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pCS3+FLT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:51 | 0 | |
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XE221 hWnt-16-pCS2P+ Resource Report Resource Website |
RRID:Addgene_16843 | hWnt-16 | Homo sapiens | Ampicillin | Description: EcoR1 fragment from a wnt16 pCDNA3 construct from Desheng Lu and Dennis A. Carson, UCSD, was subcloned into pCS2P+ Reference: Clone made by Gilbert Weidinger, Moon lab. | Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:54 | 0 | ||
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pCAG-SRGAP2A-EGFP Resource Report Resource Website |
RRID:Addgene_168501 | SRGAP2A | Homo sapiens | Ampicillin | PMID:19737524 | Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:55 | 0 | ||
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AICSDP-144:CTCF-mEGFP Resource Report Resource Website |
RRID:Addgene_168799 | CTCF | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human CTCF in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EDLYFQS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/. | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used, and WTC-11 specific homozygous SNP(s) | 2026-08-15 01:09:55 | 0 | |
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Human messenger-RBP sgRNA library Resource Report Resource Website |
RRID:Addgene_168791 | gRNA library targeting human genes | Homo sapiens | Ampicillin | PMID:34096786 | Backbone Marker:Kosuke Yusa (Addgene #67974); Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:55 | 0 | ||
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pInducer10-UBC9-sh3 Resource Report Resource Website |
RRID:Addgene_168987 | Ubiquitin Conjugating Enzyme 9 | Homo sapiens | Ampicillin | PMID:25805818 | Backbone Size:13218; Vector Backbone:pInducer-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:56 | 0 | ||
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pInducer10-UBC9-sh4 Resource Report Resource Website |
RRID:Addgene_168988 | Ubiquitin Conjugating Enzyme 9 | Homo sapiens | Ampicillin | PMID:25805818 | Backbone Size:13217; Vector Backbone:pInducer-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:09:56 | 0 |
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