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Species: Homo sapiens
Genetic Insert: GNAI1, GNB1, GNG2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34516756
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_168120 Copy
Species: Homo sapiens
Genetic Insert: GNAI2, GNB1, GNG2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34516756
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_168121 Copy
Species: Homo sapiens
Genetic Insert: GNAO1, GNB3, GNG9
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:Clontech-style N1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34516756
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.02.05.429900v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_168123 Copy
Species: Homo sapiens
Genetic Insert: NEK9
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6100; Vector Backbone:pMRX-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078910
Proper citation: RRID:Addgene_168271 Copy
Species: Homo sapiens
Genetic Insert: U1A RRM1 crystallization module
Vector Backbone Description: Backbone Marker:GenScript; Backbone Size:5400; Vector Backbone:pET28a-TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33777416
Proper citation: RRID:Addgene_168267 Copy
Species: Homo sapiens
Genetic Insert: NEK9
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6100; Vector Backbone:pMRX-IP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078910
Proper citation: RRID:Addgene_168269 Copy
Species: Homo sapiens
Genetic Insert: MLKL(1-125)
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4750; Vector Backbone:mCherry2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34540558
Proper citation: RRID:Addgene_168265 Copy
Species: Homo sapiens
Genetic Insert: Hspa8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5573; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168219 Copy
Species: Homo sapiens
Genetic Insert: Lamp2b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5578; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168209 Copy
Species: Homo sapiens
Genetic Insert: CD63
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5520; Vector Backbone:pcDNA 3.1+ Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27189348
Proper citation: RRID:Addgene_168220 Copy
Species: Homo sapiens
Genetic Insert: HA-nUbc6E-(EAAAK)4-OGT(4)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168188 Copy
Species: Homo sapiens
Genetic Insert: HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168189 Copy
Species: Homo sapiens
Genetic Insert: HA-nLag6-(EAAAK)4-OGT(4,K852A)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5364; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33950562
Proper citation: RRID:Addgene_168191 Copy
Species: Homo sapiens
Genetic Insert: flag-Chibby
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pCS3+FLT; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12712206
Comments: The full coding sequence as well as 3' UTR of Human Chibby, ~1kb, was amplified by PCR and subcloned into the EcoR1 and Xho1 sites of pCS3+FLT, providing a flag tag.
Reference: K. Takemaru et al., Nature 422: 905 (2003)
Proper citation: RRID:Addgene_16824 Copy
Species: Homo sapiens
Genetic Insert: hWnt-16
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Description: EcoR1 fragment from a wnt16 pCDNA3 construct from Desheng Lu and Dennis A. Carson, UCSD, was subcloned into pCS2P+
Reference: Clone made by Gilbert Weidinger, Moon lab.
Proper citation: RRID:Addgene_16843 Copy
Species: Homo sapiens
Genetic Insert: SRGAP2A
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19737524
Proper citation: RRID:Addgene_168501 Copy
Species: Homo sapiens
Genetic Insert: CTCF
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human CTCF in WTC-11 human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EDLYFQS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/.
Proper citation: RRID:Addgene_168799 Copy
Species: Homo sapiens
Genetic Insert: gRNA library targeting human genes
Vector Backbone Description: Backbone Marker:Kosuke Yusa (Addgene #67974); Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34096786
Proper citation: RRID:Addgene_168791 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme 9
Vector Backbone Description: Backbone Size:13218; Vector Backbone:pInducer-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_168987 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin Conjugating Enzyme 9
Vector Backbone Description: Backbone Size:13217; Vector Backbone:pInducer-10; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25805818
Proper citation: RRID:Addgene_168988 Copy
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