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Species: Mus musculus
Genetic Insert: Bicaudal D protein
Vector Backbone Description: Backbone Size:7041; Vector Backbone:pBa-eGFP-FKBP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_63569 Copy
Species: Mus musculus
Genetic Insert: Col1A-frt-hygro-pA
Vector Backbone Description: Backbone Size:2888; Vector Backbone:pBluescriptII SK; Vector Types:Mammalian Expression, Bacterial Expression, Mouse Targeting, FRT/Flpe; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26492136
Comments: The cassette in between the homologous recombination arms is the same as in AddGene Plasmid #20730.
The homologous recombination arms are derived from mouse strain FVB.
The Hygromycin gene in this construct does not have an ATG start codon nor is it expressed. Hygromycin can not be used for selection when this construct is used in a targeting experiment in mouse strain FVB derived ES cells.
The construct can be linearized with AscI, FseI or XhoI
Proper citation: RRID:Addgene_63575 Copy
Species: Mus musculus
Genetic Insert: Gcn5
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25269644
Proper citation: RRID:Addgene_63707 Copy
Species: Mus musculus
Genetic Insert: Gcn5
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25269644
Proper citation: RRID:Addgene_63708 Copy
Species: Mus musculus
Genetic Insert: HDAC3
Vector Backbone Description: Vector Backbone:pCMV5-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16982804
Proper citation: RRID:Addgene_63676 Copy
Species: Mus musculus
Genetic Insert: HDAC3
Vector Backbone Description: Backbone Size:33000; Vector Backbone:pAdeno-X; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16982804
Proper citation: RRID:Addgene_63675 Copy
Species: Mus musculus
Genetic Insert: BLBP
Vector Backbone Description: Backbone Size:1700; Vector Backbone:pCAG-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23345401
Proper citation: RRID:Addgene_63721 Copy
Species: Mus musculus
Genetic Insert: Tet1 promoter fragment A
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25582196
Proper citation: RRID:Addgene_63881 Copy
Species: Mus musculus
Genetic Insert: Tet1 promoter fragment B
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25582196
Proper citation: RRID:Addgene_63882 Copy
Species: Mus musculus
Genetic Insert: Tet1 enhancer fragment E
Vector Backbone Description: Backbone Size:5005; Vector Backbone:pGL3-Promoter; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25582196
Proper citation: RRID:Addgene_63880 Copy
Species: Mus musculus
Genetic Insert: Nanog homeobox
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22020437
Comments: Addgene's quality control sequence with EBV-rev primer shows a single bp mismatch with the available reference sequence.
Proper citation: RRID:Addgene_63893 Copy
Species: Mus musculus
Genetic Insert: Nanog homeobox
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5256; Vector Backbone:pGL3-Control; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22020437
Comments: Mir-34a binding site was mutated to remove the seed region
Proper citation: RRID:Addgene_63894 Copy
Species: Mus musculus
Genetic Insert: Pax8 paired domain
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17182173
Comments: There is a BamHI site in between the Eng and PDm coding sequences.
Drosophila Engrailed (Eng)contains H22P and L158P compared to closest reference sequence (AAA65478.1). Depositor states that these mutation should not affect function.
Mouse Pax8 contains a P2A mutation. Depositor states that this mutation should not affect plasmid function.
Proper citation: RRID:Addgene_64040 Copy
Species: Mus musculus
Genetic Insert: GLUT4
Vector Backbone Description: Backbone Size:9124; Vector Backbone:pLenti-hiko; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25575350
Comments: The c-Myc sequence was inserted into the region encoding the 1st exofacial loop of mouse GLUT4 in the background of pmCherry-N1. The fragment of Myc-GLUT4-mCherry was then sub-cloned into pLenti-hiko vector via NheI + HpaI digestion.
Discrepancies between the Addgene QC sequence and the full sequence are not of functional concern.
Please cite the following article:
Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).
Proper citation: RRID:Addgene_64049 Copy
Species: Mus musculus
Genetic Insert: bscl2
Vector Backbone Description: Vector Backbone:L309; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23458123
Proper citation: RRID:Addgene_64063 Copy
Species: Mus musculus
Genetic Insert: bscl2
Vector Backbone Description: Vector Backbone:L309; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23458123
Proper citation: RRID:Addgene_64062 Copy
Species: Mus musculus
Genetic Insert: vascular endothelial growth factor C
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pENTR/D-TOPO; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25758709
Proper citation: RRID:Addgene_64065 Copy
Species: Mus musculus
Genetic Insert: U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
Vector Backbone Description: Backbone Marker:Gene Transfer Vector Core University of Iowa; Backbone Size:5679; Vector Backbone:pacAd5; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25337876
Proper citation: RRID:Addgene_64071 Copy
Species: Mus musculus
Genetic Insert: BICD2N (1-400)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pOmniBac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:24986880
Proper citation: RRID:Addgene_64070 Copy
Species: Mus musculus
Genetic Insert: mTRF1
Vector Backbone Description: Backbone Marker:de Lange Lab (Addgene plasmid# 12540); Vector Backbone:pLPC-N MYC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25344324
Proper citation: RRID:Addgene_64162 Copy
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