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http://www.addgene.org/13985

Species: Gallus gallus
Genetic Insert: Hoxd-13
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9043077
Comments: RCASHoxd-13 was constructed using a cDNA corresponding to the longer of the two transcripts whose putative coding region is identical to the published sequence, with the exception of 9 glycines encoded by ggc repeats starting at amino acid position 10, instead of the reported 7 ggc repeats (Rogina and Upholdt, 1993). This cDNA was cloned into the shuttle vector SLAX-13 (Riddle et al., 1993), and then subcloned into the ClaI site of RCAS(BP)A .

Proper citation: RRID:Addgene_13985 Copy   

•   Source: Addgene

http://www.addgene.org/13986

Species: Gallus gallus
Genetic Insert: Hoxc6
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8812121
Comments: To generate a retrovirus capable of transcribing Hoxc-6 mRNA, the region encoding the PR11 Hoxc-6 protein was isolated from a chick cDNA clone (Nelson et al., 1996). The PR11 Hoxc-6 protein comprises 235 amino acids (Oliver et al., 1988; Coletta et al., 1991; Shimeld et al., 1993). The ATG of the PR11 coding region was engineered by PCR for cloning into the NcoI site of the SLAX-13 shuttle vector (Riddle et al., 1993), and then subcloned into the retroviral vector RCAS-BP(A) (Hughes et al., 1987). The insert is 932 base pairs and includes approximately 230 bp of 3' untranslated sequence.

Proper citation: RRID:Addgene_13986 Copy   

•   Source: Addgene

http://www.addgene.org/14015

Species: Gallus gallus
Genetic Insert: Slug
Vector Backbone Description: Backbone Size:3000; Vector Backbone:Slax13; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full length coding sequence, PCR (vent) product, 5’ primer converts 2nd codon from cca (pro) to gca (ala) in order to generate a Nco I site; 3’ primer is wt plus EcoRI site, from Malcolm Logan.

Proper citation: RRID:Addgene_14015 Copy   

•   Source: Addgene

http://www.addgene.org/14010

Species: Gallus gallus
Genetic Insert: HoxD10
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full-length Hox D10, from Craig Nelson.

Proper citation: RRID:Addgene_14010 Copy   

•   Source: Addgene

http://www.addgene.org/14024

Species: Gallus gallus
Genetic Insert: Lmx1
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBS SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For 3'UTR probe cut with Xba1 and use T7 RNA pol, from Bob Riddle.

Proper citation: RRID:Addgene_14024 Copy   

•   Source: Addgene

http://www.addgene.org/14066

Species: Gallus gallus
Genetic Insert: Chicken Beta 1 Integrin
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3487386

Proper citation: RRID:Addgene_14066 Copy   

•   Source: Addgene

http://www.addgene.org/14062

Species: Gallus gallus
Genetic Insert: Chicken Fibronectin EIIIA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2867; Vector Backbone:pGem-3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2830487

Proper citation: RRID:Addgene_14062 Copy   

•   Source: Addgene

http://www.addgene.org/83472

Species: Gallus gallus
Genetic Insert: FRNK
Vector Backbone Description: Backbone Marker:GE-Healthcare; Backbone Size:12769; Vector Backbone:TRIPZ-non-silencing shRNA control; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26216901

Proper citation: RRID:Addgene_83472 Copy   

•   Source: Addgene

http://www.addgene.org/89402

Species: Gallus gallus
Genetic Insert: CSPG5
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pGBT9; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_89402 Copy   

•   Source: Addgene

http://www.addgene.org/89412

Species: Gallus gallus
Genetic Insert: CNTN1
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_89412 Copy   

•   Source: Addgene

http://www.addgene.org/8997

Species: Gallus gallus
Genetic Insert: RSK
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11585927
Comments: Src myristylation sequence (MGSSKSKPK) lacks the polybasic domain necessary for predominant membrane localization.

Proper citation: RRID:Addgene_8997 Copy   

•   Source: Addgene

http://www.addgene.org/87232

Species: Gallus gallus
Genetic Insert: CXADR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA6/myc-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_87232 Copy   

•   Source: Addgene

http://www.addgene.org/79878

Species: Gallus gallus
Genetic Insert: Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1)
Vector Backbone Description: Backbone Size:6084; Vector Backbone:pMT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28402863

Proper citation: RRID:Addgene_79878 Copy   

•   Source: Addgene

http://www.addgene.org/80024

Species: Gallus gallus
Genetic Insert: vinculin
Vector Backbone Description: Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23468527

Proper citation: RRID:Addgene_80024 Copy   

•   Source: Addgene

http://www.addgene.org/80169

Species: Gallus gallus
Genetic Insert: segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
Vector Backbone Description: Vector Backbone:Bluescript KS+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16925019
Comments: Please note that there are some discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

Proper citation: RRID:Addgene_80169 Copy   

•   Source: Addgene

http://www.addgene.org/80428

Species: Gallus gallus
Genetic Insert: E2F transcription factor 4
Vector Backbone Description: Vector Backbone:pcDNA6/V5-His-A2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22586272

Proper citation: RRID:Addgene_80428 Copy   

•   Source: Addgene

http://www.addgene.org/92359

Species: Gallus gallus
Genetic Insert: chick U6.3 promoter and gRNA cloning cassette
Vector Backbone Description: Backbone Size:3017; Vector Backbone:pNG3; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:29386245
Comments: Kudo, T., and S. Sutou. 2005. Usage of putative chicken U6 promoters for vector-based RNA interference. J Reprod Dev. 51:411-417. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 Please visit https://www.biorxiv.org/content/early/2017/05/08/135525 for bioRxiv preprint.

Proper citation: RRID:Addgene_92359 Copy   

•   Source: Addgene

http://www.addgene.org/45061

Species: Gallus gallus
Genetic Insert: NgCam
Vector Backbone Description: Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839364
Comments: NgCam is very GC rich and difficult to sequence and PCR. pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains. Addgene's sequencing results found a few nucleotide differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositor, these differences are not a concern for the function of the plasmid.

Proper citation: RRID:Addgene_45061 Copy   

•   Source: Addgene

http://www.addgene.org/45554

Species: Gallus gallus
Genetic Insert: Hoxa7
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23359544

Proper citation: RRID:Addgene_45554 Copy   

•   Source: Addgene

http://www.addgene.org/45796

Species: Gallus gallus
Genetic Insert: TN-XXL
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19160515
Comments: TN-XXL is composed of two fluorescent proteins: cyan fluorescent protein (CFP) as the FRET donor and cpCitrine as the FRET acceptor. These proteins are linked by the calcium-sensitive double C-terminal lobe of troponin C (TnC). After the binding of free calcium, TnC undergoes a reversible conformational change that leads to energy transfer from the donor to the acceptor fluorophore, resulting in a drop in CFP fluorescence and an increase in cpCitrine fluorescence.

Proper citation: RRID:Addgene_45796 Copy   

•   Source: Addgene