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Species: Homo sapiens
Genetic Insert: CAD
Vector Backbone Description: Backbone Marker:This study; Vector Backbone:pLenti-EF1alpha-DEST-IRES-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253785 Copy
Species: Homo sapiens
Genetic Insert: CAD
Vector Backbone Description: Backbone Marker:This study; Vector Backbone:pLenti-EF1alpha-DEST-IRES-Neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42056505
Comments: Please visit https://doi.org/10.1101/2025.07.23.666448 for bioRxiv preprint.
Proper citation: RRID:Addgene_253786 Copy
Species: Synthetic
Genetic Insert: HaloTag-TEV-transmembrane domain-mCherry
Vector Backbone Description: Vector Backbone:pBIN20; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42086881
Comments: Please see the following preprints as well as their final/published form:
Lowensohn, T. N.; Cody, W. B.; Tsai, C.; Vlahos, A. E.; Call, C. C.; Gao, X. J.; Sattely, E. S. An in Planta Single-Cell Screen to Accelerate Functional Genetics. bioRxiv February 19, 2026, p 2025.08.06.668818. https://doi.org/10.1101/2025.08.06.668818.
Vlahos, A. E.; Call, C. C.; Eckman, N.; Kadaba, S. E.; Guo, S.; Thurm, A. R.; Kang, J.; Appel, E. A.; Bintu, L.; Gao, X. J. A Complete Suite for Compact Programming of Protein Secretion. bioRxiv May 27, 2025, p 2023.10.04.560774. https://doi.org/10.1101/2023.10.04.560774.
Proper citation: RRID:Addgene_254126 Copy
Species: Other
Genetic Insert: ABR, lv0, Akan, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_249985 Copy
Species: Other
Genetic Insert: dropout part, lv0*, 1R-5O, sfGFP
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250039 Copy
Species: Synthetic
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42754580
Comments: Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint.
Proper citation: RRID:Addgene_247430 Copy
Species: Other
Genetic Insert: dropout part, lv0*, 1C-5C, mScarlet-I
Vector Backbone Description: Backbone Marker:Georg Fritz and Thorsten Mascher; Vector Backbone:Synthetic; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40650977
Proper citation: RRID:Addgene_250040 Copy
Species: Homo sapiens
Genetic Insert: DDX28 pre sequence
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4011; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42026137
Comments: Please visit https://doi.org/10.1101/2025.06.06.658068 for bioRxiv preprint.
Proper citation: RRID:Addgene_254615 Copy
Species: Homo sapiens
Genetic Insert: DDX28
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4011; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42026137
Comments: Please visit https://doi.org/10.1101/2025.06.06.658068 for bioRxiv preprint.
Proper citation: RRID:Addgene_254616 Copy
Species: Synthetic
Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:pET29; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42754580
Comments: Please visit https://doi.org/10.1101/2025.08.05.668824 for bioRxiv preprint.
Proper citation: RRID:Addgene_247379 Copy
Genetic Insert: GFP_AID*
Vector Backbone Description: Backbone Marker:Andreas Milias-Argeitis, Addgene Plasmid #180285 as described by Novarina et al. (2022).; Vector Backbone:pYTK-DN4; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: The golden gate (Multi-gene) plasmid assembly was performed according to the MoClo-Cloning standards described in: Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A highly characterized yeast toolkit for modular, multipart assembly. ACS synthetic biology, 4(9), 975-986.
Parts from the MoClo Yeast Toolkit (Addgene kit #1000000061) were used for MoClo assembly.
The OsTIR1(F74G, S210A) variant was originally described in Xing et al., 2025 (https://doi.org/10.1038/s41467-025-61848-1).
Proper citation: RRID:Addgene_253756 Copy
Species: Other
Genetic Insert: dBa1Cas12a2 E1032A C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253636 Copy
Genetic Insert: GFP_AID*
Vector Backbone Description: Backbone Marker:Andreas Milias-Argeitis, Addgene Plasmid #180285 as described by Novarina et al. (2022).; Vector Backbone:pYTK-DN4; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Comments: The golden gate (Multi-gene) plasmid assembly was performed according to the MoClo-Cloning standards described in: Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A highly characterized yeast toolkit for modular, multipart assembly. ACS synthetic biology, 4(9), 975-986.
Parts from the MoClo Yeast Toolkit (Addgene kit #1000000061) were used for MoClo assembly.
The OsTIR1(F74G, S210A) variant was originally described in Xing et al., 2025 (https://doi.org/10.1038/s41467-025-61848-1).
Proper citation: RRID:Addgene_253758 Copy
Species: Other
Genetic Insert: ca23Cas12a3 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Backbone Marker:Twist; Vector Backbone:pET-29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253638 Copy
Species: Other
Genetic Insert: MpCas12a2 E. coli codon optimized C-terminal His tag
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41501459
Proper citation: RRID:Addgene_253633 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Merck/Novagen; Vector Backbone:pET24d; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42025218
Comments: YSD 2.0 Toolkit Plasmid. Please visit https://doi.org/10.1101/2025.08.19.671047 for bioRxiv preprint.
Proper citation: RRID:Addgene_253056 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Vector Backbone:pNIC28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40685547
Comments: The His tag can be removed by TEV.
Proper citation: RRID:Addgene_253392 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Vector Backbone:pET-47b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40685547
Comments: The His tag can be removed by HRV 3C.
Proper citation: RRID:Addgene_253393 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Vector Backbone:pET-47b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40685547
Comments: The His tag can be removed by HRV 3C.
Proper citation: RRID:Addgene_253394 Copy
Species: Homo sapiens
Genetic Insert: Ubiquitin
Vector Backbone Description: Vector Backbone:pET-24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40685547
Comments: The His tag cannot be removed.
Proper citation: RRID:Addgene_253397 Copy
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