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Vector Backbone Description: Backbone Marker:Vieira and Messing, 1991; Backbone Size:3868; Vector Backbone:pUK21; Vector Types:Bacterial Expression, Suicide vector for R. rubrum; Bacterial Resistance:Kanamycin
Comments: Constructed by D. P. Lies and G. P. Roberts
Proper citation: RRID:Addgene_36328 Copy
Genetic Insert: shRNA of scramble
Vector Backbone Description: Backbone Marker:Biosettia; Backbone Size:10275; Vector Backbone:pLV-H1TetO-RFP-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Comments: The plasmid backbone is TetO-H1-shRNA-EF1a-TetR-IRES-RFP-Puro. It is a all-in-one inducible shRNA knockdown lentiviral plasmid.
The paper associated with this plasmid is "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling"
Proper citation: RRID:Addgene_36311 Copy
Species: Mus musculus
Genetic Insert: mTERT
Vector Backbone Description: Backbone Marker:Bob Weinberg (Addgene Plasmid #1764); Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21732358
Proper citation: RRID:Addgene_36413 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Plasmid carrying monomeric (A206K) EGFP for N-terminal tagging
Proper citation: RRID:Addgene_36412 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: Made by mutagenesis directly on plasmid Bcr-ECFP using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N689/ E690 in Bcr. We therefore mutated these residues in Bcr and introduced the mutants into the RAW 264.7 macrophage cell line. However, the construct did not caused morphological changes in these cells. Constructed by Young-Jin Cho.
Proper citation: RRID:Addgene_36418 Copy
Species: Homo sapiens
Genetic Insert: EHD1 EH domain
Vector Backbone Description: Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_36459 Copy
Species: Homo sapiens
Genetic Insert: autophagy-related protein 2 homolog A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22219374
Proper citation: RRID:Addgene_36456 Copy
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36436 Copy
Species: Homo sapiens
Genetic Insert: RECQL4-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGCGGGACGTGCGGGAGC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36679 Copy
Species: Homo sapiens
Genetic Insert: HOXA13-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCCGTGCTCCTCCACCCC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36671 Copy
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:8508; Vector Backbone:pJFRC-MUH; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22493255
Proper citation: RRID:Addgene_36431 Copy
Species: Homo sapiens
Genetic Insert: FANCA-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGGCCCGAGGCGGAGTTC
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36818 Copy
Species: Homo sapiens
Genetic Insert: PTEN-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TCCTTTTGTTTCTGCTAA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36765 Copy
Species: Homo sapiens
Genetic Insert: HDAC6-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TTCGCTGCCTGGTTGTGG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36804 Copy
Species: Homo sapiens
Genetic Insert: ATM-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGAATTGGGATGCTGTTT
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36805 Copy
Species: Homo sapiens
Genetic Insert: ATM-TALEN_Right
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32285); Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TAAAGACAGTAAAATAAA
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36806 Copy
Species: Homo sapiens
Genetic Insert: HDAC2-TALEN_Left
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22484455
Comments: Target binding site: TGCGCTCACCTCCCTGCG
It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb.
Proper citation: RRID:Addgene_36829 Copy
Genetic Insert: T (i.e., ATG, start codon as a first exon)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Comments: The first exon (T) that contains the start codon is out of frame with the second GFP exon.
Proper citation: RRID:Addgene_36887 Copy
Genetic Insert: T (i.e., ATG, start codon as a first exon)
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Comments: ATG (T) and GFP are not in frame.
Proper citation: RRID:Addgene_36889 Copy
Genetic Insert: tTA2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22479386
Proper citation: RRID:Addgene_36880 Copy
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