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Species: E. coli
Genetic Insert: FLIP-E A207W
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5325; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15939876
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
Proper citation: RRID:Addgene_13548 Copy
Species: E. coli
Genetic Insert: FLIP-E WT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5325; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15939876
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
Proper citation: RRID:Addgene_13545 Copy
Species: E. coli
Genetic Insert: FLIP-E A207R
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5325; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15939876
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
Proper citation: RRID:Addgene_13546 Copy
Species: E. coli
Genetic Insert: FLIPglu F16A G275S-ECFP-K276R
Vector Backbone Description: Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16131659
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
Citrine is modified version of EYFP.
Proper citation: RRID:Addgene_13565 Copy
Species: E. coli
Genetic Insert: FLIPglu F16A Y12S-ECFP-D13R
Vector Backbone Description: Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16131659
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
Citrine is modified version of EYFP.
Proper citation: RRID:Addgene_13563 Copy
Species: E. coli
Genetic Insert: FLIPglu F16A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12649277
Comments: For more information, see
http://dpbweb.stanford.edu/sites/www.dpb.ciw.edu/files/frommer/nanosensors/index.html
Proper citation: RRID:Addgene_13572 Copy
Species: E. coli
Genetic Insert: FLIPglu F16A Delta11 Aphrodite
Vector Backbone Description: Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16935985
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
EYFP was exchanged with Aphrodite (accession number DQ525025), which is an codon-diversified version of Venus/EYFP. Ares (accession number DQ525024) was exchanged for ECFP, which is an codon-diversified version of ECFP.
Proper citation: RRID:Addgene_13571 Copy
Species: E. coli
Genetic Insert: FLIPglu F16A Delta11 Aphrodite
Vector Backbone Description: Backbone Size:2897; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16935985
Comments: For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
EYFP was exchanged with Aphrodite (accession number DQ525025), which is an codon-diversified version of Venus/EYFP.
Proper citation: RRID:Addgene_13569 Copy
Species: E. coli
Genetic Insert: microcin-V bacteriocin cassette
Vector Backbone Description: Backbone Marker:Oxford Genetics; Backbone Size:4700; Vector Backbone:pSF_UC_OXB20-daGFP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30954530
Comments: Original mccV cassette from:
Gilson, L., Mahanty, H.K. and Kolter, R., 1987. Four plasmid genes are required for colicin V synthesis, export, and immunity. Journal of bacteriology, 169(6), pp.2466-2470.
PMID: 3034857
Depositor confirms N112D mutation in the first insert does not affect function of the plasmid.
Proper citation: RRID:Addgene_133307 Copy
Species: E. coli
Genetic Insert: hok/sok toxin-antitoxin cassette
Vector Backbone Description: Backbone Marker:Oxford Genetics; Backbone Size:4700; Vector Backbone:pSF_UC_OXB20-daGFP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30954530
Proper citation: RRID:Addgene_133305 Copy
Species: E. coli
Genetic Insert: His-MinC
Vector Backbone Description: Backbone Size:5245; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21516096
Proper citation: RRID:Addgene_133628 Copy
Species: E. coli
Genetic Insert: mCherryGGBsMTS
Vector Backbone Description: Backbone Size:5258; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30258191
Proper citation: RRID:Addgene_133625 Copy
Species: E. coli
Genetic Insert: mCherry-2xEcMTS
Vector Backbone Description: Backbone Size:5258; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30258191
Proper citation: RRID:Addgene_133624 Copy
Species: E. coli
Genetic Insert: MinE-His
Vector Backbone Description: Backbone Size:5206; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30571913
Proper citation: RRID:Addgene_133623 Copy
Species: E. coli
Genetic Insert: EGFP-MinD
Vector Backbone Description: Backbone Size:5260; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24937143
Proper citation: RRID:Addgene_133622 Copy
Species: E. coli
Genetic Insert: His-MinD_MinE
Vector Backbone Description: Backbone Size:5260; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18467587
Proper citation: RRID:Addgene_133621 Copy
Species: E. coli
Genetic Insert: Maltose-binding protein (MalE)
Vector Backbone Description: Backbone Marker:IBA GmbH; Backbone Size:3286; Vector Backbone:pASK-IBA2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30600549
Comments: Spycatcher was published in Zakeri et al. (2012), PNAS 109, ppE690-7
Proper citation: RRID:Addgene_107422 Copy
Species: E. coli
Genetic Insert: rpoA-rpoB-rpoC-6xHis and rpoZ
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25665556
Comments: Plasmid pVS10 encodes the E. coli rpoA-rpoB-rpoC [His6] and
rpoZ ORFs under control of T7 promoter.
Proper citation: RRID:Addgene_104398 Copy
Species: E. coli
Genetic Insert: rpoA, B, C, and Z
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET; Vector Types:Bacterial Expression, E. coli expression vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25665556
Comments: pIA900 (rpoA, B, C, and Z) encodes a C-terminally His-tagged β′ which can be removed using TEV protease. Linker with TEV-His10 cloned between NheI and StuI sites of pIA787; NheI site is gone
Proper citation: RRID:Addgene_104401 Copy
Species: E. coli
Genetic Insert: RecE residues 564-866 (C-terminal nuclease domain)
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19446525
Proper citation: RRID:Addgene_104534 Copy
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