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Species: Homo sapiens
Genetic Insert: Autotaxin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:pSecTag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327261
Comments: From the depositor:
In 1998, we purchased from Invitrogen pSecTag2A and cut it with BglII and BamH1. The BglII/BamH1 fragment was replaced with a modified fragment containing 6 Histidines at the N-Terminus that was done by generating the modification by PCR, and cloning the PCR product back into BglII/BamH1.
Sometime later, the above pSecTag2A-6HIS was digested with BamH1and Not1. The ATX (amino acids 52-863) insert was genereated by PCR, digested with BamH1/EcoR1 and by excising ATX from the original colony II cDNA library with EcoR1/Not1, which yields two pieces in two stages into the vector.
Their is a stop codon before the C-Terminus Myc tag so it will not be expressed.
Proper citation: RRID:Addgene_17839 Copy
Species: Homo sapiens
Genetic Insert: T210A-ATX
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:pSecTag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18327261
Proper citation: RRID:Addgene_17840 Copy
Species: Synthetic
Genetic Insert: jGCaMP7b-XC
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36196992
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.01.09.475579v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_178361 Copy
Species: Discosoma sp., A. victoria
Genetic Insert: E. coli optimized red flourescent protein (RFPec), GFPuv
Vector Backbone Description: Backbone Marker:Groningen Biotechnology Center; Backbone Size:0; Vector Backbone:pBAD24; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16845378
Proper citation: RRID:Addgene_17827 Copy
Species: Mus musculus
Genetic Insert: pAAV hSyn iGluSnFR3 v857.SGZ
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pAAV hSyn; Vector Types:Mammalian Expression, Mouse Targeting, AAV, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37142767
Proper citation: RRID:Addgene_178330 Copy
Species: Homo sapiens
Genetic Insert: shSHP2
Vector Backbone Description: Backbone Marker:Chen lab; Backbone Size:8200; Vector Backbone:MDH1-404-H2Kb2; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17382377
Comments: Target region of SHP-2: TTGCCCACGCATATCATGTAGT (NM_011202.3: 5421 to 5442, 3’ UTR).
Proper citation: RRID:Addgene_17854 Copy
Species: Homo sapiens
Genetic Insert: NEUROD1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34582787
Proper citation: RRID:Addgene_178582 Copy
Species: Mus musculus
Genetic Insert: Stargazin-mEGFP-iLID
Vector Backbone Description: Backbone Size:4720; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34880255
Comments: The sequence and plasmid map is available in the following benchling link (https://benchling.com/s/seq-XUhPNN7CavWnbGAq1TUe).
Proper citation: RRID:Addgene_178523 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12535517
Comments: This clone was generated by my postdoctoral felllow: Xavier Espanel.
Proper citation: RRID:Addgene_17843 Copy
Species: Homo sapiens
Genetic Insert: YAP
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4700; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12535517
Comments: This clone was generated by my postdoctoral felllow: Akihiko Komuro.
Proper citation: RRID:Addgene_17844 Copy
Species: Quail
Genetic Insert: TVA950 Glu66→Thr
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV, Cre/Lox, Adeno-associated virus; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_178430 Copy
Species: Homo sapiens
Genetic Insert: GGA1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12686608
Comments: Please note: Plasmid contains a S434L mutation in GGA1 compared to the NCBI reference sequence NP_037497.1. This mutation is not known to affect plasmid function.
Proper citation: RRID:Addgene_178459 Copy
Species: Homo sapiens
Genetic Insert: BATF
Vector Backbone Description: Vector Backbone:pFUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35245086
Proper citation: RRID:Addgene_178451 Copy
Species: Homo sapiens
Genetic Insert: STAT2
Vector Backbone Description: Vector Backbone:pFUW-TetO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35245086
Proper citation: RRID:Addgene_178449 Copy
Species: Other
Genetic Insert: miRE-Ptbp1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34582787
Proper citation: RRID:Addgene_178589 Copy
Species: E. coli
Genetic Insert: FLII12Pglu-700uDelta6
Vector Backbone Description: Backbone Size:0; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18177733
Proper citation: RRID:Addgene_17867 Copy
Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34582787
Proper citation: RRID:Addgene_178583 Copy
Species: Mus musculus
Genetic Insert: NFATc3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11439183
Comments: Full-length mNFATc3
Proper citation: RRID:Addgene_17868 Copy
Species: Mus musculus
Genetic Insert: CnA alpha
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pBJ5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1377362
Proper citation: RRID:Addgene_17871 Copy
Species: Bos taurus
Genetic Insert: GRK2
Vector Backbone Description: Backbone Size:9173; Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35045279
Proper citation: RRID:Addgene_178734 Copy
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