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Species: Homo sapiens
Genetic Insert: CBX7 Chromo
Vector Backbone Description: Vector Backbone:pGEX-6p-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25301795
Proper citation: RRID:Addgene_59695 Copy
Species: Homo sapiens
Genetic Insert: zonula occludens-1
Vector Backbone Description: Backbone Marker:EMD Millipore; Backbone Size:4670; Vector Backbone:pET14b_TEV; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_59739 Copy
Species: Homo sapiens
Genetic Insert: PML isoform IV
Vector Backbone Description: Backbone Size:4713; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24882209
Proper citation: RRID:Addgene_59742 Copy
Vector Backbone Description: Vector Backbone:pGL4.11; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_59744 Copy
Species: Mus musculus
Genetic Insert: ELYS-emerin-EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_59745 Copy
Species: Mus musculus
Genetic Insert: LBR-EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_59748 Copy
Species: Mus musculus
Genetic Insert: emerin-EGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_59747 Copy
Species: Mus musculus
Genetic Insert: LBR-mRFP1
Vector Backbone Description: Vector Backbone:pcDNA3.1-polyA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24908396
Proper citation: RRID:Addgene_59749 Copy
Genetic Insert: attL1-em7-kanR-attL2-pA
Vector Backbone Description: Vector Backbone:pBluescript SKII-; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24924516
Proper citation: RRID:Addgene_59764 Copy
Species: Synthetic
Genetic Insert: CAS9
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23997119
Comments: The eGFP ORF was inserted into C-terminal of CAS9 ORF of
pCAG-T3-hCAS-pA (Addgene Plasmid #48625).
For the in vitro synthesis of CAS9 mRNAs, the vector was linearized by SphI and in-vitro transcribed using T3-RNA-polymerase (Promega) in the presence of m7G(5′)ppp(5′)G to synthesize capped RNA.
The Tbpl1 3′UTR 95 nucleotide polyA tail at the 3' end of the insert allows in vitro transcribed mRNA from this plasmid to be used directly for microinjection into animal embryos or oocytes without additional polyadenylation treatment.
The CAG promoter in this plasmid also allows it to be used as a CAS9eGFP-expression vector.
Proper citation: RRID:Addgene_59766 Copy
Species: Homo sapiens
Genetic Insert: RBFOX2
Vector Backbone Description: Backbone Size:13918; Vector Backbone:pSLIK Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24637117
Proper citation: RRID:Addgene_59769 Copy
Species: Synthetic
Genetic Insert: mRuby2_smFP_FLAG
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Proper citation: RRID:Addgene_59760 Copy
Species: Synthetic
Genetic Insert: mRuby2_smFP_OLLAS
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25915120
Proper citation: RRID:Addgene_59761 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Comments: Please note that some batches of CspCI from New England Biolabs may cut inefficiently and alters the mobility of the DNA, possibly because it remains bound to the target. The two batches that are known to work are 0041303 and 0051407. This may be due to the particular target sequence present in pMZ283 and pMZ374.
Proper citation: RRID:Addgene_59896 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Proper citation: RRID:Addgene_59898 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:6215; Vector Backbone:pMZ283; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25352017
Proper citation: RRID:Addgene_59897 Copy
Species: Homo sapiens
Genetic Insert: Bub1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20124418
Proper citation: RRID:Addgene_59812 Copy
Species: Synthetic
Genetic Insert: mCherry with intron containing the mouse mir-124–3
Vector Backbone Description: Vector Backbone:pcDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847681
Comments: To create a repressive regulatory link between the miRNA and the mCherry transcript, we added a truncated version of the mir-124-regulated 3′UTR of the Vamp3 gene to the mRNA. This 3′UTR contains one 6-mer and three 7-mer target sites complementary to the mir-124 seed region (nucleotides 2−8 from the 5′ end of the miRNA). To better observe the dynamics of gene expression, we destabilized the mCherry protein using a standard PEST degradation tag.
Proper citation: RRID:Addgene_59892 Copy
Species: Synthetic
Genetic Insert: mCherry with intron containing the mouse mir-124–3
Vector Backbone Description: Vector Backbone:pcDNA5 FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24847681
Comments: To create a repressive regulatory link between the miRNA and the mCherry transcript, we added a truncated version of the mir-124-regulated 3′UTR of the Vamp3 gene to the mRNA. This 3′UTR contains one 6-mer and three 7-mer target sites complementary to the mir-124 seed region (nucleotides 2−8 from the 5′ end of the miRNA). To better observe the dynamics of gene expression, we destabilized the mCherry protein using a standard PEST degradation tag.
Proper citation: RRID:Addgene_59894 Copy
Species: Caenorhabditis elegans
Genetic Insert: rab-3 promoter
Vector Backbone Description: Vector Backbone:pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25377555
Proper citation: RRID:Addgene_59788 Copy
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