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Species: Escherichia coli
Genetic Insert: Ec86 RT
Vector Backbone Description: Backbone Marker:Fraser lab; Vector Backbone:pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35076284
Proper citation: RRID:Addgene_170185 Copy
Species: Bradyrhizobium sp. (strain ORS 278)
Genetic Insert: NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
Vector Backbone Description: Backbone Marker:Originally derived from pEGFP-N1 backbone after several modifications in our lab.; Backbone Size:3427; Vector Backbone:pQP-Select; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34162879
Comments: To design iLight, a full-length bacterial phytochrome IsPadC from Idiomarina sp. A28L was used as an original template. First, diguanylyl cyclase effector in IsPadC was removed, then following mutations were introduced: I68F, H80Q, A86T, R90S S242C, E274K, R295H I360V, and L464V. After that, iLight was human codon optimized for mammalian expression.
Proper citation: RRID:Addgene_170273 Copy
Species: Homo sapiens
Genetic Insert: SEAP
Vector Backbone Description: Backbone Marker:GeneScript; Backbone Size:4108; Vector Backbone:pG12; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34162879
Proper citation: RRID:Addgene_170271 Copy
Species: Xenopus laevis
Genetic Insert: C-cadherin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pSP36T; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7556920
Comments: Linearize with EcoRI and transcribe with SP6.
A cDNA encoding Xenopus C-cadherin was cloned by screening a Xenopus lambda gt10 oocyte cDNA library. The cDNA was instered into pSP36T for transcription using the SP6 promoter.
Proper citation: RRID:Addgene_17023 Copy
Genetic Insert: slc1a3b
Vector Backbone Description: Backbone Size:2807; Vector Backbone:p5E; Vector Types:Unspecified, Zebrafish; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32895565
Proper citation: RRID:Addgene_170205 Copy
Species: Danio rerio
Genetic Insert: slc1a3b
Vector Backbone Description: Backbone Size:3299; Vector Backbone:pT2A; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32895565
Proper citation: RRID:Addgene_170208 Copy
Species: Homo sapiens
Genetic Insert: YFP(ST)-PH
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11152673
Proper citation: RRID:Addgene_170354 Copy
Species: Homo sapiens
Genetic Insert: mT2Del_EPACdDEPCD_tdcp173Ven(ST)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25875503
Proper citation: RRID:Addgene_170349 Copy
Species: Homo sapiens
Genetic Insert: mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25875503
Proper citation: RRID:Addgene_170348 Copy
Genetic Insert: Bst-LF-Idaho
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_170469 Copy
Species: SARS-CoV-2
Genetic Insert: Nucleocapsid
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.10.459410v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_170466 Copy
Species: Synthetic
Genetic Insert: iRFP670-CaaX
Vector Backbone Description: Vector Backbone:pHR-SIN; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33988299
Proper citation: RRID:Addgene_170464 Copy
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33644903
Proper citation: RRID:Addgene_170367 Copy
Species: Synthetic
Genetic Insert: flex H2B GFP
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33644903
Proper citation: RRID:Addgene_170368 Copy
Species: Synthetic
Genetic Insert: H2B-RFP
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33644903
Proper citation: RRID:Addgene_170363 Copy
Species: Homo sapiens
Genetic Insert: Cas9-T2A-GFP
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31320637
Proper citation: RRID:Addgene_170361 Copy
Species: Homo sapiens
Genetic Insert: solute carrier family 7 member 11
Vector Backbone Description: Backbone Size:8687; Vector Backbone:plenti6.3-V5; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28630042
Proper citation: RRID:Addgene_170427 Copy
Species: Synthetic
Genetic Insert: hU6-BB-LacZ and EF1A-EGFP-2A-Puro
Vector Backbone Description: Backbone Size:7466; Vector Backbone:3rd Generation lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34050182
Proper citation: RRID:Addgene_170459 Copy
Species: Mus musculus
Genetic Insert: GluA2
Vector Backbone Description: Backbone Size:9370; Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33514725
Proper citation: RRID:Addgene_170454 Copy
Species: MERS
Genetic Insert: MERS spike D12
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32540903
Proper citation: RRID:Addgene_170448 Copy
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