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Species: Mus musculus
Genetic Insert: cFOS
Vector Backbone Description: Backbone Size:3000; Vector Backbone:BSSK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_19259 Copy
Species: Mus musculus
Genetic Insert: mouse Wrn cDNA
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_19274 Copy
Species: Mus musculus
Genetic Insert: ForminI Exon 9
Vector Backbone Description: Backbone Size:4900; Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16480715
Proper citation: RRID:Addgene_19322 Copy
Species: Mus musculus
Genetic Insert: FmnI Iso1b
Vector Backbone Description: Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16480715
Proper citation: RRID:Addgene_19323 Copy
Species: Mus musculus
Genetic Insert: DNAJB10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Family: HSP40
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19479 Copy
Species: Mus musculus
Genetic Insert: DNAJB3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Family: HSP40
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19471 Copy
Species: Mus musculus
Genetic Insert: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
Vector Backbone Description: Backbone Size:34864; Vector Backbone:pAd CMV/V5 DEST; Vector Types:Adenoviral, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18754011
Comments: Addgene was unable to acquire sequence for this plasmid but is distributing the plasmid at the Melton Lab's request. Please address any problems with this plasmid to the Melton lab.
Proper citation: RRID:Addgene_19412 Copy
Species: Mus musculus
Genetic Insert: 14-3-3 zeta
Vector Backbone Description: Backbone Size:6100; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9665134
Proper citation: RRID:Addgene_1943 Copy
Species: Mus musculus
Genetic Insert: MST1
Vector Backbone Description: Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12384512
Proper citation: RRID:Addgene_1965 Copy
Species: Mus musculus
Genetic Insert: DnaJ (Hsp40) homolog, subfamily B, member 3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18686016
Comments: Part of the HSP40 gene family
Disclaimer:
For cloning the various HSP genes, the Stratagene reference RNA for cDNA synthesis was used and genes were amplified from this mixture that originates from 10 different cell lines from different somatic origin and ultimately coming from 10 different individuals. This strategy was used to maximize the chance for the presence of individual HSP transcripts. Users must be aware that this mixture thus represents 20 complete haploid genomes, meaning that for each individual gene there might be polymorphisms that could potentially have functional consequences. The library was sequence verified only for the presence of the correct gene.
Proper citation: RRID:Addgene_19552 Copy
Species: Mus musculus
Genetic Insert: alpha myosin heavy chain promoter
Vector Backbone Description: Backbone Size:7233; Vector Backbone:SMPU; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19352491
Proper citation: RRID:Addgene_21231 Copy
Species: Mus musculus
Genetic Insert: PKC gamma
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21236 Copy
Species: Mus musculus
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21243 Copy
Species: Mus musculus
Genetic Insert: PKC eta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.
Please note that Addgene was not able to completely confirm the truncation with quality control sequencing.
Proper citation: RRID:Addgene_21247 Copy
Species: Mus musculus
Genetic Insert: PKC eta
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Comments: Osada, S., Mizuno, K., Saido, T.C., Akita, Y., Suzuki, K., Kuroki, T., and Ohno, S. 1990. A phorbol ester receptor/protein kinase, nPKC eta, a new member of the protein kinase C family predominantly expressed in lung and skin. The Journal of biological chemistry 265(36): 22434-22440.
Proper citation: RRID:Addgene_21245 Copy
Species: Mus musculus
Genetic Insert: PKC epsilon
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12794082
Proper citation: RRID:Addgene_21240 Copy
Species: Mus musculus
Genetic Insert: alpha myosin heavy chain promoter
Vector Backbone Description: Backbone Size:8400; Vector Backbone:SIN18; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19352491
Proper citation: RRID:Addgene_21229 Copy
Species: Mus musculus
Genetic Insert: alpha myosin heavy chain promoter
Vector Backbone Description: Backbone Size:7338; Vector Backbone:SMPU; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19352491
Comments: There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a "C" adjacent to "GGG". The construct should be correct.
Proper citation: RRID:Addgene_21228 Copy
Species: Mus musculus
Genetic Insert: Axin2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:For riboprobe synthesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11809808
Comments: Tong DNA #94. The DNA is a HinDIII fragment of mouse Axin2 (nucleotides 1-2397) in pBluescript-KS. The antisense and sense Axin2 riboprobes were made by linearizing this plasmid with NotI or SalI and transcribing with T3 or T7 RNA polymerase, respectively. List of DNAs says " ~2 kb DIII fragment of Axin2 (1-2397) in pBS-KS”.
Proper citation: RRID:Addgene_21277 Copy
Species: Mus musculus
Genetic Insert: Axin2 promoter
Vector Backbone Description: Backbone Marker:Clontech (discontinued); Backbone Size:4300; Vector Backbone:d2EGFP-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11809808
Comments: The 5.6-kb DNA fragment was excised from Axin2-Luciferase using KpnI and HindIII (the HindIII site was filled in with Klenow DNA polymerase) and cloned into the KpnI- and SmaI-digested pd2EGFP-1 vector.
Proper citation: RRID:Addgene_21276 Copy
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