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Species: E. coli
Genetic Insert: b-lactamase
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2642; Vector Backbone:modified pGPS3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11602576
Comments: Positive control for mutagenesis experiments: reversion of the stop codon results in ampicillin resistance.
The pGPS3 has a deletion from BglI to BglII that destroys the ampicillin resistance gene. The ampicillin resistance gene containing the stop codon was then cloned in between AflIII and KpnI.
Please also see pGFPuv from BD Biosciences as another control for forward mutation assays. Catalog # 6079-1 http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins_by_Name/GFP_GFPuv_Fluorescent_Proteins?sitex=10020:22372:US .
Proper citation: RRID:Addgene_11724 Copy
Species: Cas9 (Streptococcus pyogenes M1 GAS)
Genetic Insert: Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
Vector Backbone Description: Backbone Marker:Sheng Yang, Addgene Plasmid #62225; Backbone Size:4476; Vector Backbone:pCas; Vector Types:other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30927506
Proper citation: RRID:Addgene_117259 Copy
Species: Homo sapiens
Genetic Insert: PSD2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117463 Copy
Species: Homo sapiens
Genetic Insert: Cyth3
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117454 Copy
Species: Homo sapiens
Genetic Insert: Cyth4
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117456 Copy
Species: Homo sapiens
Genetic Insert: Iqsec1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117444 Copy
Species: Homo sapiens
Genetic Insert: NMT2
Vector Backbone Description: Vector Backbone:pMON; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11771383
Comments: This plasmid also contains the gene that expresses E. coli methionine aminopeptidase under its own promoter
Proper citation: RRID:Addgene_117477 Copy
Species: Homo sapiens
Genetic Insert: NMT1
Vector Backbone Description: Vector Backbone:pMON; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11771383
Comments: This plasmid also contains the gene that expresses E. coli methionine aminopeptidase under its own promoter
Proper citation: RRID:Addgene_117476 Copy
Vector Backbone Description: Backbone Marker:Yanisch-Perron et al.; Backbone Size:2860; Vector Backbone:pTZ18U; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9514705
Proper citation: RRID:Addgene_117478 Copy
Species: Homo sapiens
Genetic Insert: PSD4
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117473 Copy
Species: Homo sapiens
Genetic Insert: PSD2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_117466 Copy
Species: Synthetic
Genetic Insert: [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117642 Copy
Species: Synthetic
Genetic Insert: [35S:Sp-xCas9 3.7(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117641 Copy
Species: Synthetic
Genetic Insert: [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117644 Copy
Species: Synthetic
Genetic Insert: [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117643 Copy
Species: Synthetic
Genetic Insert: [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117647 Copy
Species: Synthetic
Genetic Insert: [35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117631 Copy
Species: Synthetic
Genetic Insert: [35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117597 Copy
Species: Synthetic
Genetic Insert: [35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117632 Copy
Species: Synthetic
Genetic Insert: [35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
Vector Backbone Description: Vector Backbone:pICSL4723; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117594 Copy
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